High-pressure liquid-chromatographic method for determination of gentamicin in plasma.

Abstract

A rapid, specific method for measuring gentamicin in plasma by high-pressure liquid chromatography was developed. After deproteinization, gentamicin in the supernate was dansylated and extracted into ethyl acetate. The organic extract was chromatographed on a microparticulate reversed-phase column, which was eluted with aqueous acetonitrile. Use of the dansyl derivative enables fluorometry, for more sensitive quantitation. Various factors that could affect the assay sensitivity were investigated. With 0.2-ml plasma samples, the method can accurately measure as little as 1 mg of gentamicin per liter. We encountered no interferences from plasma supplemented with various drugs or plasma of patients who were on therapy with other drugs. This method can also separate gentamicin C1 from C1a and C2, all of which are present in various ratios in commercial dosage forms. This method is also applicable to gentamicin determination in urine.

Terbium-Macrocycle Complexes as Chemical Sensors: Detection of an Aspirin Metabolite in Urine Using a Salicylurate-Specific Receptor Site

“…Other HPLC techniques report sensitivities of 0.2 mg/L of SA in urine 7 and 0.5 mg/L of SA in plasma. 14 A liquid chromatographic method with UV detection has a sensitivity of 0.5 mg/L with a precision of 8.6 mg/L but takes 25 min and requires purification steps. 15 Capillary electrophoresis coupled to laser-induced fluorescence has also been described to detect SA, SU, and other metabolites in urine following sample filtration and dilution.…”

Determination of Kanamycin Plasma Levels Using LC-MS and Its Pharmacokinetics in Patients

“…The plasma concentration-time profile for each patient was plotted using a semi-log graph paper. Kanamycin PK parameters were calculated based on the non-compartmental analysis [22][23][24] and expressed as median and range (lowest to highest).…”

“…The LC-MS method is a proven method and has been widely used in the quantification of anti-TB drugs [22][23][24]. The plasma levels of kanamycin were determined using LC-MS method and analysis was conducted according to scientific standards.…”

“…High peak plasma concentration of aminoglycosides is associated with increased rate of elimination of Mycobacterium tuberculosis (MTB) and better therapeutic response in patients [4,12]. However, due to the narrow therapeutic range (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35) µg/ml of aminoglycosides [12], care must be taken when administering kanamycin in patients with renal impairment.…”

Mass spectrometric study to characterize thioisoindole derivatives of aminoglycoside antibiotics

“…For instance, the detection of gentamicin was accomplished with fluorescence detection after post‐column derivatization with 1,2‐phthalic dicarboxaldehyde (OPA) 9, 10. Similarly, liquid chromatography (LC) with pre‐column derivatization by OPA11 and dansyl chloride12 followed by fluorescence detection was performed for gentamicin analysis. Freeman et al 13.…”