High-pressure liquid chromatography analysis of antibiotic susceptibility disks

Hagel, Robert B.; Waysek, Edward H.; Cort, Winifred M.

doi:10.1128/aac.16.3.372

Cited by 15 publications

(5 citation statements)

References 8 publications

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“…The methodology used in this study to measure the serum concentrations of the antibiotic is based on the diffusion of the antibiotic in agar plates and the determination of diameters in inhibition halos [ 23 ]. This method was implemented because it is cheap, fast, and reproducible for our medium compared to chromatographic techniques such as HPLC [ 23 , 24 ]. In the pharmacokinetic analysis of these data, the assumption that the patients were in a stationary state was followed, and an infusion time of 30 min used for this group of patients was also considered.…”

Section: Discussionmentioning

confidence: 99%

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Pharmacokinetics and Pharmacodynamics of Cefepime in Adults with Hematological Malignancies and Febrile Neutropenia after Chemotherapy

et al. 2021

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Patients with chemotherapy-induced febrile neutropenia (CIFN) may have changes in the pharmacokinetics (PK) compared to patients without malignancies or neutropenia. Those changes in antibiotic PK could lead to negative outcomes for patients if the therapy is not adequately adjusted to this. In this, open-label, non-randomized, prospective, observational, and descriptive study, a PK model of cefepime was developed for patients with hematological neoplasms and post-chemotherapy febrile neutropenia. This study was conducted at a cancer referral center, and study participants were receiving 2 g IV doses of cefepime every 8 h as 30-min infusions. Cefepime PK was well described by a two compartment model with a clearance dependent on a serum creatinine level. Using Monte Carlo simulations, it was shown that continuous infusions of 6g q24h could have a good achievement of PK/PD targets for MIC levels below the resistance cut-off point of Enterobacteriaceae. According to the simulations, it is unnecessary to increase the daily dose of cefepime (above 6 g daily) to increase the probability of target attainment (PTA). Cumulative fraction of response (CFR) using interment dosing was suboptimal for empirical therapy regimens against K. pneumoniae and P. aeruginosa, and continuous infusions could be used in this setting to maximize exposure. Patients with high serum creatinine levels were more likely to achieve predefined PK/PD targets than patients with low levels.

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Section: Discussionmentioning

confidence: 99%

“…The determination of plasma concentration of cefepime was carried out by means of microbiological assay [ 23 , 24 ]. The diameters of the growth inhibition halos of Bacillus subtillis strain ATCC 6633 were measured.…”

Section: Materials and Methodsmentioning

confidence: 99%

Pharmacokinetics and Pharmacodynamics of Cefepime in Adults with Hematological Malignancies and Febrile Neutropenia after Chemotherapy

et al. 2021

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“…[7][8][9] Nowadays, there are many methods for the determination of CET, including high-performance liquid chromatography (HPLC), infrared Raman spectroscopy (IR-Raman), colorimetry, spectrophotometry, and so forth. [10][11][12][13][14] However, the above methods have some shortcomings, such as expensive equipment, time-consuming, complex operations, and so on, which are not conducive to real-time monitoring. Therefore, it is decisively significant to develop an exact, portable, and calculable CET detection method.…”

Section: Introductionmentioning

confidence: 99%

A highly selective Cd‐based metal‐organic framework fluorescent probe for rapid recognition of cephalothin in water

Wang,

Zhu,

Tan

et al. 2024

Applied Organom Chemis

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Nowadays, the problem of antibiotic pollution in the water environment is becoming more and more serious, which has caused great harm to human health and the circulatory system. Therefore, it is extremely crucial to design an effective and sensitive method for the recognition of antibiotics in water. In this research, 1‐(4‐carboxyphenyl)‐1H‐pyrazole‐3‐carboxylic acid (H2L) was used as a ligand to prepare a luminescent two‐dimensional (2D) metal–organic framework (MOF), CdL·H2O (1). 1 can specifically recognize antibiotic cephalothin (CET) in aqueous environments through a fluorescence‐enhanced response, which demonstrated a favorable linear correlation between the fluorescence intensities of 1 and the concentrations of CET (0–50 μM) with a linear fluorescence enhancement slope KEC value of 5.4750 × 104 M−1 and a limit of detection (LOD) of 165 nM. The fast response, good selectivity, anti‐interference ability, and reusability for CET recognition were also verified. 1 represents the inaugural MOF‐based fluorescent material designed for the recognition of CET. Additionally, the detection mechanism for CET was explored in detail with a view to better understanding the fluorescence sensing process.

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“…The advantage of the HPLC analysis method is that one can get the results in a very specific manner (Snyder et al, 1997). The application of HPLC for the analysis of AST discs of various antibiotics, mecillinam, ampicillin, carbenicillin, and cephalothin, is reported (Hagel et al, 1979). The authors have described potency determination of AST disc of mecillinam, ampicillin, carbenicillin, and cephalothin alone and in combinations.…”

Section: Introductionmentioning

confidence: 99%

Ensuring robustness in the quality of antibiotic susceptibility test discs for four novel antibiotics

Ahirrao

Jadhav

Vaidya

et al. 2022

Biomedical Chromatography

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Antibiotic susceptibility test (AST) discs are used as an in-vitro diagnostic tool to select the appropriate antibiotic to treat an infection. Generally, the concentration of the drug loaded on to the AST discs is measured by studying its activity against quality control organisms. This methodology has several limitations-it is time consuming, requires trained manpower, has a wider acceptance criteria of zone of inhibitionscausing ambiguity in judging smaller variations in drug concentration. To overcome these issues, we have developed and validated high-performance liquid chromatographic (HPLC) methods for the determination of strength of AST discs for in-house researched antibiotics, namely Levonadifloxacin/WCK 771, Nafithromycin/WCK 4873, Cefepime-Tazobactam/WCK 4282, and Cefepime-Zidebactam/WCK 5222.The drugs were extracted from the AST discs using an appropriate solvent. The developed methods are simple, accurate, precise, reproducible, rugged, and robust.They are efficient in terms of time, and can be easily conducted in a quality control laboratory during release as well as stability evaluation of AST disc. Application of HPLC methods for the determination of strength of AST discs ensures flawless quality and, consequently, a better selection of drugs to treat bacterial infections in clinics.

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High-pressure liquid chromatography analysis of antibiotic susceptibility disks

Cited by 15 publications

References 8 publications

Pharmacokinetics and Pharmacodynamics of Cefepime in Adults with Hematological Malignancies and Febrile Neutropenia after Chemotherapy

Pharmacokinetics and Pharmacodynamics of Cefepime in Adults with Hematological Malignancies and Febrile Neutropenia after Chemotherapy

A highly selective Cd‐based metal‐organic framework fluorescent probe for rapid recognition of cephalothin in water

Ensuring robustness in the quality of antibiotic susceptibility test discs for four novel antibiotics

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