High prevalence, low counts and uncommon serotypes of Listeria monocytogenes in linguiça, a Brazilian fresh pork sausage

Miyasaki, Keila Naomi; Chiarini, Eb; ́Ana, Anderson de Souza Sant; Destro, Maria Teresa; Landgraf, Mariza; Franco, Bernadette Dora Gombossy de Melo

doi:10.1016/j.meatsci.2009.06.037

Cited by 24 publications

(21 citation statements)

References 55 publications

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“…Although our results are similar to those of the United Kingdom, the differences in the data related to occurrence and distribution of the genus Listeria might be explained by the geographical/environmental specificity [29]. L. monocytogenes was also found to be more prevalent than L. innocua in food products in warm/hot countries, such as Brazil [30], India [31], and Mexico [32]. …”

Section: Resultssupporting

confidence: 68%

Listeriaspp. in Street-Vended Ready-to-Eat Foods

El‐Shenawy

El-Shenawy

Mañes

et al. 2011

Interdisciplinary Perspectives on Infectious Diseases

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Street-vended ready-to-eat food sold in Egypt, including sandwiches and dishes of traditional food, was examined for the presence of Listeria species. Out of 576 samples, 24% were found to contain Listeria species. L. monocytogenes and L. innocua were isolated from 57% and 39% of the contaminated samples, respectively. Other Listeria spp. were detected with lower frequency. L. monocytogenes of ≥103 CFU/g were detected in 7% of the total examined samples, which represent 49% of the contaminated food samples (meat, poultry, seafood, dairy products, and products of plant origin). Most of the samples contaminated by L. monocytogenes had high levels of total viable bacterial counts. The results obtained may help to clarify the epidemiology of listeriosis in the country and draw the attention of the decision makers to issue hygienic regulations for food processing industries as well as street vendors in order to ensure safe street-vended ready-to-eat food.

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Section: Resultssupporting

confidence: 68%

Listeriaspp. in Street-Vended Ready-to-Eat Foods

El‐Shenawy

El-Shenawy

Mañes

et al. 2011

Interdisciplinary Perspectives on Infectious Diseases

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“…Other studies also reported diverse results related to the characterization of L. monocytogenes using molecular and conventional methods (Miyasaki et al . ; Vanegas et al . ; Gambarin et al .…”

Section: Discussionmentioning

confidence: 99%

“…Miyasaki et al . () reported PCR identification of L. monocytogenes in 29% of fresh pork sausage samples, while the positivity for the pathogen was 42% by the ISO method.…”

Section: Discussionmentioning

confidence: 99%

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Prevalence, Serotype Identification by Multiplex Polymerase Chain Reaction and Antimicrobial Resistance Patterns of Listeria Monocytogenes Isolated from Retail Foods

Çetinkaya

Muş

Yibar

et al. 2014

Journal of Food Safety

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In the present study, a total of 512 food samples composed of raw milk, dairy products, meat/meat products, chicken meat, seafood and raw vegetables were analyzed for the presence of Listeria monocytogenes. The results of the standard identification methods showed that 20 (3.9%) of the analyzed samples were found to harbor this pathogen. Further, 8.4% (13/155) of chicken meats, 0.9% (1/105) of meat/meat products and 13.6% (6/44) of fresh vegetables were contaminated with L. monocytogenes. Interestingly, only 18 of these isolates gave expected band size when they were subjected to molecular confirmation by polymerase chain reaction (PCR). Multiplex PCR serotyping of the strains revealed that 66.6% (12/18) of which belonged to serotype 1/2a (or 3a), 5.6% (1/18) to serotype 1/2b (or 3b, 7), 5.6% (1/18) to serotype 1/2c (or 3c) and 11.1% (2/18) to serotype 4b (or 4d, 4e). Two strains could not be serotyped by multiplex PCR. The strains were also evaluated by disk diffusion assay for their susceptibility to 15 commonly used antimicrobials. Antimicrobial resistance was most frequently observed for clindamycin (94.4%), followed by streptomycin and kanamycin (88.9%); penicillin (72.2%), tetracycline and gentamicin (66.7%); quinopristin/dalfopristin and erythromycin (61.1). Interestingly, 13 strains were resistant to more than five antibiotics. All strains were susceptible to linezolid, teicoplanin and vancomycin. Practical Applications Listeria monocytogenes is one of the most important foodborne pathogens responsible for several outbreaks and cases of listeriosis in human. This study focused on the prevalence of L. monocytogenes in different raw and ready‐to‐eat foodstuffs, and serotype distribution among the isolates. Antibiotic resistance profiles of the isolates were also reported. Information and relief provided to consumers could help elaborate public health and food safety.

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“…On the other hand, the non-invasive form is associated with fever and gastrointestinal symptoms with no complications (Aureli et al, 2000;Pichler et al, 2009). Up to date 13 potentially pathogenic serovars of L. monocytogenes have been identified, with 4b (37 -64%), 1/2b and 1/2a (15 -25%), and 1/2c (0 -4%) the most frequent outbreaks and sporadic cases of the disease (Jacquet et al, 1995;Jay, 1996;Miyasaki et al, 2009;Markkula et al, 2011). The population identified at risk for this disease include immunocompromised individuals, pregnant women, infants and adults over 65 years (López et al, 2007;Garmendia et al, 2008;Mammina et al, 2009;Taillefer et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Risk factors favoring the presence of Listeria monocytogenes in Colombian pork-meat processing plants

Ana¹,

Diana²,

Viviana³

et al. 2014

Afr. J. Microbiol. Res.

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We tracked Listeria monocytogenes as a microbiological risk factor in pork processing plants. The five plants analyzed were located in main areas for meat processing in Colombia. Prevalence of L. monocytogenes was 11.46% (36/314), represented by serotypes 4b (6/36, 16.7%), 4d/4e (10/36, 27.8%), 4b/4d/4e (4/36; 11.1%), 3a (3/36, 8.3%), 3c (1/36, 2.8) and 1/2c/3c (9/36, 25%); 4/36 isolates (11.1%) were assumed as possible serotypes 4ab or 7. There was no tolerance to per-acetic acid and only 9/36 (25%) surface-isolates displayed tolerance (0.5-2% (v/v)) to alkyl dimethyl benzyl ammonium chloride. The risk factors analysis showed variations for each processing plant and found that surface and equipment had a higher degree of contamination, with the chopper and cutter at the highest risk. The general prevalence of L. monocytogenes in pork meat processing plants was 11.46%. All these results demonstrate deficiencies in the implementation and monitoring of cleaning and disinfection programs.

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High prevalence, low counts and uncommon serotypes of Listeria monocytogenes in linguiça, a Brazilian fresh pork sausage

Cited by 24 publications

References 55 publications

Listeriaspp. in Street-Vended Ready-to-Eat Foods

Listeriaspp. in Street-Vended Ready-to-Eat Foods

Prevalence, Serotype Identification by Multiplex Polymerase Chain Reaction and Antimicrobial Resistance Patterns of Listeria Monocytogenes Isolated from Retail Foods

Risk factors favoring the presence of Listeria monocytogenes in Colombian pork-meat processing plants

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