High Prevalence of Antibiotic Resistance in Pathogenic Escherichia coli from Large- and Small-Scale Poultry Farms in Bangladesh

Hasan, Badrul; Faruque, Rayhan; Drobni, Mirva; Waldenström, Jonas; Sadique, Abdus; Ahmed, Kabir Uddin; Islam, Zahirul; Parvez, M. B. Hossain; Olsen, Bjørn R.; Alam, Munirul

doi:10.1637/9892-968611-digest.1

Cited by 16 publications

(33 citation statements)

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“…Antibiotic-resistant bacteria, including ESBL-producing Enterobacteriaceae, have been detected in food animals, meats, water, and the environment in many Asian countries, including China, Hong Kong, India, Bangladesh, and Malaysia, and could be reservoirs for colonization and infection of human beings. [18][19][20][21][22][23][24][25][26][27][28] There are several previous microbiological surveys of food animals on farms, fresh meat samples from slaughterhouses and retail stores, exported fresh meat samples and vegetables, and stool samples from both healthy individuals and hospital patients from Thailand and several other countries. [29][30][31][32][33][34][35][36][37][38][39][40][41] Almost all of these reports emphasized the ability of foodborne bacteria in causing gastrointestinal infections, especially Salmonella, Campylobacter, and Arcobacter species, though only a few studies described antibiotic-resistant E. coli.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of antibiotic resistant bacteria in healthy adults, foods, food animals, and the environment in selected areas in Thailand

Boonyasiri

Tangkoskul

Seenama

et al. 2014

Pathogens and Global Health

132

110

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Objectives: The aim of this study was to determine the prevalence of antibiotic-resistant bacteria, especially extended-spectrum beta-lactamase (ESBL) producing Escherichia coli, in samples from healthy adults, foods, food animals, and the environment in selected areas of Thailand. Methods: Samples were collected from stool specimens from adult food factory and food animal farm workers, fresh and cooked foods sold at markets, rectal swabs of healthy pigs and chickens, fresh pork meat from slaughterhouses, water samples from canals as well as fish and shrimp farm ponds, and stagnant water sources on pig farms. Antibiotic susceptibility was determined using the disk diffusion or agar dilution methods. Extended-spectrum beta-lactamase production was assayed using a double disk diffusion method. Results: Among 544 healthy adult food factory workers, 75.5% were positive for ESBL producing E. coli, while 77.3% of E. coli isolated from 30 healthy animal farm workers were positive. Amongst healthy food animals, ESBL producing status among E. coli isolates were more commonly detected in pigs (76.7%) than broilers (40%). Extended-spectrum beta-lactamase producing E. coli seemed to be more prevalent in fresh meat samples than in fresh vegetables, in fresh foods than in cooked foods, and in water samples collected from the animal farms than those from canals and fish and shrimp ponds. Conclusions: Extended-spectrum beta-lactamase producing E. coli isolates are prevalent amongst healthy individuals, foods along the food production chain from farms to consumers, and in the environment in selected areas in Thailand.

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Section: Introductionmentioning

confidence: 99%

Prevalence of antibiotic resistant bacteria in healthy adults, foods, food animals, and the environment in selected areas in Thailand

Boonyasiri

Tangkoskul

Seenama

et al. 2014

Pathogens and Global Health

132

110

View full text Add to dashboard Cite

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“…In previous studies carried out on E. coli from poultry environment in Bangladesh showed resistance to penicillin (i.e. amoxicillin and ampicillin) in a range of 28%-88% and to tetracycline 45.5% to 58% [7,8]. Though lowest level of resistance (30.8%) was found against nitrofurantoin for E. coli of chicken origin, but it is still a matter of concern that how these isolates become resistant to this broad spectrum of antibiotic which is mostly used for human treatment purpose.…”

Section: Discussionmentioning

confidence: 98%

“…Among the various sectors in Bangladesh, poultry industry is one of the sectors where antibiotic misuse is common and thereby, have generated selection pressure towards antimicrobial resistance [6]. In fact, current data of Bangladesh have indicated presence of resistant and ESBL producing bacteria among poultry and household birds [7,8]. Recently in different countries poultry has been identified as a potential reservoir of resistant bacteria and transfer of resistance from non-pathogenic bacteria to pathogens and vice versa can happen [9,10].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Inhibitor Resistant Beta Lactamase Producing E. coli in Human and Poultry Origin of Bangladesh

Parvez¹,

Marzan²

2016

J Bacteriol Parasitol

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Human clinical specimens (n=48) and poultry fecal specimens (n=40) were collected from a sub district (Savar) of Bangladesh. Total 25 E. coli were isolated from these specimens. These E. coli were tested for their antibiotic sensitivity against commonly used antibiotics. In addition, to detect the ESBL (extended spectrum beta lactamases) producers, double disk synergy (DDS) test and PCR method were used. Twenty three E. coli were multidrug resistant, i.e. resistant against at least three different groups of antibiotics. DDS method showed all E. coli from poultry specimens and 78% of E. coli from clinical specimens were resistant to amoxicillin-clavulanic acid combination. Thus they are phenotypically confirmed as inhibitor resistant beta lactamase producers. PCR result showed only blaTEMgene in E. coli from poultry specimens. Thus inhibitor resistant type β-lactamase was found to be dominant in E. coli from both types of specimens.

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“…The main goal is to reduce the level of APEC exposure by reducing any stress related factors and the number of APEC in the chicken's production environment (feed, drinking water, litter, dust) (Barnes, 2013). This goal can be achieved by improving husbandry and biosecurity, ventilation, nutrition, and immune status among the chicken flocks (Dhillon and Jack, 1996;Hasan et al, 2011;Nolan et al, 2013). Recommendations include the addition of chlorine into drinking water to reduce the introduction and spread of disease and decrease the mortality rates associated with APEC outbreaks (Vandekerchove et al, 2004).…”

Section: Management Procedures To Control And/or Prevent Avian Colibamentioning

confidence: 99%

“…High tetracycline resistance (39.5% -56.8%) in E. coli isolates sourced from chickens has been documented in Australia Barton and Wilkins, 2001;Obeng et al, 2012) as well as, globally (Hasan et al, 2011;Saenz et al, 2001). Obeng et al (2012) …”

Section: Tetracyclinementioning

confidence: 99%

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

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High Prevalence of Antibiotic Resistance in Pathogenic Escherichia coli from Large- and Small-Scale Poultry Farms in Bangladesh

Cited by 16 publications

References 0 publications

Prevalence of antibiotic resistant bacteria in healthy adults, foods, food animals, and the environment in selected areas in Thailand

Prevalence of antibiotic resistant bacteria in healthy adults, foods, food animals, and the environment in selected areas in Thailand

Prevalence of Inhibitor Resistant Beta Lactamase Producing E. coli in Human and Poultry Origin of Bangladesh

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

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