High prevalence of Campylobacter jejuni CC21 and CC257 clonal complexes in children with gastroenteritis in Tehran, Iran

Sarhangi, Mahnaz; Bakhshi, Bita; Peeraeyeh, Shahin Najar

doi:10.21203/rs.3.rs-20388/v2

Cited by 1 publication

(1 citation statement)

References 32 publications

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“…Although one isolate in our collection was from ST-45 CC, this host generalist clonal complex is often one of the most commonly isolates lineages in clinical surveillance studies worldwide (De Haan et al, 2010; Sheppard et al, 2009; Shin et al, 2013; Sopwith et al, 2008). Notably however, it is often absent (or under-represented) in studies conducted in LMICs (Pascoe et al, 2020; Sarhangi et al, 2021). This is consistent with observations from other LMICs, where local differences in disease epidemiology are reflected by the absence of common Campylobacter lineages, and the presence of rare or unique sequence types (Graham et al, 2016; Pascoe et al, 2020; Prachantasena et al, 2016; P.…”

Section: Discussionmentioning

confidence: 99%

Local accessory gene sharing drives lineage-specific acquisition of antimicrobial resistance in Egyptian Campylobacter spp.

Mouftah

Pascoe

Calland

et al. 2021

Preprint

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Campylobacter is the most common cause of bacterial gastroenteritis worldwide and diarrheal disease is a major cause of child morbidity, growth faltering and mortality in low- and middle-income countries (LMICs). Despite evidence of high incidence and differences in disease epidemiology, there is limited genomic data from studies in developing countries. In this study, we characterized the genetic diversity and accessory genome content of a collection of Campylobacter isolates from Cairo, Egypt. In total, 112 Campylobacter isolates were collected from broiler carcasses (n=31), milk and dairy products (n=24) and patients (n=57) suffering from gastroenteritis. Among the most common sequence types (STs) we identified were the globally disseminated, host generalist ST-21 clonal complex (CC21) and the poultry specialist CC206, CC464 and CC48. Notably, CC45 and the cattle-specialist CC42 were under-represented with a total absence of CC61. Comparative genomics were used to quantify core and accessory genome sharing among isolates from the same country compared to sharing between countries. Lineage-specific accessory genome sharing was significantly higher among isolates from the same country, particularly CC21 which demonstrated greater local geographical clustering. In contrast, no geographic clustering was noted in either the core or accessory genomes of the CC828, suggesting a highly admixed population. A greater proportion of C. coli isolates were multidrug resistant (MDR) compared to C. jejuni. This is a significant public health concern as MDR food chain pathogens are difficult to treat and often pose increased mortality risk demanding enhanced prevention strategies in the Egyptian market to combat such a threat.

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