High prevalence of carbapenemase, AmpC β-lactamase and aminoglycoside resistance genes in extended-spectrum β-lactamase-positive uropathogens from Northern India

Gajamer, Varsha Rani; Bhattacharjee, Amitabha; Paul, Deepjyoti; Ingti, Birson; Sarkar, Aniruddha; Kapil, Jyotsna; Singh, Ashish Kumar; Pradhan, Nilu; Tiwari, Hare Krishna

doi:10.1016/j.jgar.2019.07.029

Cited by 30 publications

(21 citation statements)

References 33 publications

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“…Among the tested antibiotics, only amikacin, piperacillin-tazobactam, vancomycin, and fosfomycin had acceptable sensitivity (<20%) (Table 3 , 5 ). This prevalence pattern is similar to the comprehensive review by Mortazavi-Tabatabaei et al [ 19 ] from Iran; Gajamer et al [ 11 ]; and Shah et al [ 6 ] from India (Table 5 ). Muhammad et al [ 16 ] had recently reported a much higher prevalence of resistance for all antibiotics in Pakistan as compared to our study, with even carbapenem resistance reaching 40% (Table 5 ).…”

Section: Discussionsupporting

confidence: 89%

“…Muhammad et al [ 16 ] had recently reported a much higher prevalence of resistance for all antibiotics in Pakistan as compared to our study, with even carbapenem resistance reaching 40% (Table 5 ). The increased prevalence of carbapenem resistance in our study and similar geographical regions could be due to the higher prevalence of carbapenemase genes (97%) among the ESBL isolates, as reported by Gajamer et al, from India in 2019 [ 11 ]. On the other hand, Sokhn et al from Lebanon had reported a much lesser prevalence of resistance for various antibiotics in their recent analysis (Table 5 ) [ 24 ].…”

Section: Discussionsupporting

confidence: 69%

“…The calculated sample size was a minimum of 238 samples for each study period with an absolute precision of 5%(0.05), and an estimated prevalence rate of 19% (0.19) based on an earlier study from this region [ 11 ]. The sample size was calculated using the following formula:…”

Section: Methodsmentioning

confidence: 99%

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Antimicrobial Resistance Among Uropathogens: Surveillance Report From South India

Ravishankar¹,

P²,

Premamalini³

2021

Cureus

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 69%

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Antimicrobial Resistance Among Uropathogens: Surveillance Report From South India

Ravishankar¹,

P²,

Premamalini³

2021

Cureus

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“…Class 1, 2 and 3 integrons and oxacillinase resistance genes assay Genomic DNA of Enterobacter spp. was extracted by the simple boiling method as previously described [20]. The presence of potential resistance genes encoding class 1, 2 and 3 integrons and oxacillinase, including intI1, intI2, intI3 integrases and bla oxa−1 , bla oxa−2 , bla oxa−10 and bla oxa−48 were screened by PCR ampli cation using speci c previously reported primers [21][22][23][24][25].…”

Section: Carbapenemase Phenotypic Detectionmentioning

confidence: 99%

Emergence of OXA-10 and OXA-48 like carbapenemases among Enterobacter isolates from inpatients in southern Iran

Moradi

Khashei

Malekzadegan

et al. 2021

Preprint

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Background The global spread of carbapenemase-producing Enterobacteriaceae represents a public health concern. The aim of this study was to investigate prevalence of carbapenem resistance, oxacillinase types and the presence of class 1–3 integrons among Enterobacter clinical isolates from an Iranian inpatients’ population. Methods Ninety Enterobacter isolates recovered from hospitalized patients were diagnosed by the standard microbiological methods. Antibiogram pattern was also determined. The presence of class 1–3 integrons and four types of oxacillinase genes were assessed using PCR. Results Of the 90 Enterobacter isolates, the most common species was identified as E. aerogenes, (45.6%), followed by E. cloacae (30%). The highest resistance rate was against to ampicillin (96.7%). Multi-drug resistance (MDR) was substantial (93%). Carbapenemase producers were detected in 96% of carbapenem resistant isolates by mCIM test. The frequency of evaluated genes was as follows: intI1 = 50 (55.6%), intI2 = 12 (13.3%), blaoxa−1 =6 (6.7%), blaoxa−2 =5 (5.6%), blaoxa−10 =18 (20%), and blaoxa−48 =18 (20%). Conclusion The determinants of class 1 integron with OXA-10 and OXA-48 like carbapememases have been responsible of relatively considerable of carbapenem resistance among isolates. This is the first OXA-10 and OXA-48-producing Enterobacter spp. in Iran, indicating that the prevalence of oxacillinases might be on the rise in country.

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“…Their increasing use in recent years has facilitated the emergence of quinolone-resistant uropathogens, which makes the treatment of infections caused by extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL) very limited 12 . Since ESBL genes are often encoded in plasmids, where they can also carry genes that encode resistance to other classes of antimicrobials, including quinolones 13 . Thus, it has been reported that resistance to quinolones is increasing, although these resistance rates show significant geographical variations 14 15 .…”

Section: Introductionmentioning

confidence: 99%

Quinolone resistance in different uropathogens isolated from urine cultures in patients with urinary tract infection

Villegas

Carrillo

Contreras

et al. 2020

Preprint

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ObjectiveTo identify patterns of resistance against quinolones in various uropathogens in urinary tract infections in the population of the Toluca valley, MexicoIntroductionQuinolones are antibiotics with a spectrum of activity for both gram-positives and gram-negatives and are antibiotics used for the empirical treatment of urinary tract infections. Recently, a high index of resistance to quinolones has been reported due to different mechanisms on the part of bacteria, however the one that has taken the greatest importance is the presence of extended spectrum beta-lactamasesMethods155 samples were collected from patients with suspected urinary tract infection without exclusion criteria such as age or gender. Automated equipment was used for the identification of the etiological agent and sensitivity tests to quinolones.ResultsThe results positives were divided to evaluate which of the two antibiotics studied had greater resistance. For ciprofloxacin there are 27 resistant strains 37%, 1 strain with intermediate resistance 1% and 45 susceptible strains 62%. For levofloxacin 26 strains are resistant 36%, 41 strains are sensitive 56% and 6 strains show intermediate sensitivity 8%.ConclusionDifferent microorganisms, both gram-positive and gram-negative, were isolated and it can be observed that gram-negative strains are the ones with the greatest resistance against quinolones, mainly Escherichia coli, which produces extended-spectrum beta-lactamases, in the case of gram-positive resistance patterns are variable with a tendency towards sensitivity.

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High prevalence of carbapenemase, AmpC β-lactamase and aminoglycoside resistance genes in extended-spectrum β-lactamase-positive uropathogens from Northern India

Cited by 30 publications

References 33 publications

Antimicrobial Resistance Among Uropathogens: Surveillance Report From South India

Antimicrobial Resistance Among Uropathogens: Surveillance Report From South India

Emergence of OXA-10 and OXA-48 like carbapenemases among Enterobacter isolates from inpatients in southern Iran

Quinolone resistance in different uropathogens isolated from urine cultures in patients with urinary tract infection

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