High prevalence of Enterocytozoon hepatopenaei in shrimps Penaeus monodon and Litopenaeus vannamei sampled from slow growth ponds in India

Biju, Narayanan; Sathiyaraj, Ganesan; Raj, Mithun; Shanmugam, Venu; Baskaran, Babu; Govindan, Umamaheswari; Kumaresan, Gayathri; Kasthuriraju, Karthick Kannan; Chellamma, Thampi Sam Raj Yohannan

doi:10.3354/dao03036

Cited by 52 publications

(29 citation statements)

References 4 publications

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“…As early as the end of the nineteenth century, French researchers reported that Thelohania infects Palaemon rectirostris, P. serratus, Crangon vulgaris, Procambarus clarkii, and Astacus fluviatilis as well as other shrimp (Stentiford and Dunn, 2014). Reportedly, the infection rate as well as the transmission range of Enterocytozoon hepatopenaei (EHP) in Litopenaeus vannamei has been increasing annually, making it one of three major pathogens endangering healthy L. vannamei cultures (Biju et al, 2016;Chaijarasphong et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Development of a Quantitative PCR Method for Specific and Quantitative Detection of Enterocytospora artemiae, a Microsporidian Parasite of Chinese Grass Shrimp (Palaemonetes sinensis)

et al. 2021

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Enterocytospora artemiae (EAM) mainly parasitizes the hepatopancreas of Palaemonetes sinensis. Serious infection leads to hepatopancreatic lesions, which greatly reduce the vitality of P. sinensis. Currently, EAM is detected via conventional PCR methods. However, conventional PCR has low sensitivity and cannot be used for accurate quantitative detection of EAM or its parasitic activity in host tissues. In this study, we designed a pair of specific primers based on the sequence of the ribosomal protein S9 gene (RPS9; GenBank accession number: MZ420734) to establish and optimize a SYBR Green I real-time fluorescent quantitative PCR detection method for EAM. Only EAM appeared as a bright and single target band, whereas other microorganisms did not, indicating that the primer for RPS9 had high specificity. This method displayed optimum amplification effects at an annealing temperature of 55°C, and the melting curve of the product produced a single peak. The established method showed a good linear relationship from 2.2 × 108 to 2.2 × 101 copies/μL. The relationship between the number of cycle thresholds (Ct) and the logarithm of the initial template amount (x) conformed to Ct = −3.281 log x + 36.543 (R2 = 0.998). Amplification efficiency was 101.737%, and the lower limit of detection sensitivity was 2.2 × 101 copies/μL. Good intra- and inter-group repeatability was observed within the linear range. The sensitivity of this method was more than 200 times higher than that of nested PCR. Thus, detection data obtained using this method may be useful as a technical reference for rapid and accurate identification of EAM infection and for the prevention and control of EAM during P. sinensis breeding.

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Section: Introductionmentioning

confidence: 99%

Development of a Quantitative PCR Method for Specific and Quantitative Detection of Enterocytospora artemiae, a Microsporidian Parasite of Chinese Grass Shrimp (Palaemonetes sinensis)

et al. 2021

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“…In case of hepatopancreas samples from juveniles, the prevalence rate was moderately low (20.58%). But in the earlier studies conducted by Rajendran et al, (2016) and Biju et al, (2016) the prevalence was high at a rate of 63.5% and 69% respectively. As reported by Thitamadee et al, (2016) E. hepatopenaei causing hepatopancreatic microsporidiosis is confined to tubule epithelial cells of the hepatopancreas.…”

Section: Figure1 Pcr Amplification Of Spore Wall Protein Gene Of Enterocytozoon Hepatopenaeimentioning

confidence: 77%

A Report on the Hepatopancreatic Microsporidiosis Caused by Enterocytozoon hepatopenaei (EHP) in Penaeus vannamei (Pacific White Shrimp) Farms in Thiruvallur District, Tamilnadu, India

Giridharan¹,

Uma²

2017

Int.J.Curr.Microbiol.App.Sci

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“…An earlier study by Rajendran et al, (2016) has documented a lower prevalence a rate of 63.5% by the nested PCR. However, higher prevalence was documented by Biju et al, (2016) at a rate of 69%. The prevalence of EHP in the hepatopancreatic tissue of P. vanammei was recorded around 66.66 % which is comparatively higher that the earlier report (Giridharan and Uma., 2017).…”

Section: Locationmentioning

confidence: 89%

Prevalence of Diseases caused by White Spot Virus and Enterocytozoon Hepatopenaei in Penaeus vannamei Shrimp Farms in Nagapattinam District, Tamil Nadu, India

Rathipriya¹,

Anand²,

Uma³

et al. 2019

Int.J.Curr.Microbiol.App.Sci

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High prevalence of Enterocytozoon hepatopenaei in shrimps Penaeus monodon and Litopenaeus vannamei sampled from slow growth ponds in India

Cited by 52 publications

References 4 publications

Development of a Quantitative PCR Method for Specific and Quantitative Detection of Enterocytospora artemiae, a Microsporidian Parasite of Chinese Grass Shrimp (Palaemonetes sinensis)

Development of a Quantitative PCR Method for Specific and Quantitative Detection of Enterocytospora artemiae, a Microsporidian Parasite of Chinese Grass Shrimp (Palaemonetes sinensis)

A Report on the Hepatopancreatic Microsporidiosis Caused by Enterocytozoon hepatopenaei (EHP) in Penaeus vannamei (Pacific White Shrimp) Farms in Thiruvallur District, Tamilnadu, India

Prevalence of Diseases caused by White Spot Virus and Enterocytozoon Hepatopenaei in Penaeus vannamei Shrimp Farms in Nagapattinam District, Tamil Nadu, India

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