High prevalence of Escherichia coli co-harboring conjugative plasmids with colistin- and carbapenem resistance genes in a wastewater treatment plant in China

Wang, Di; Zou, Huiyun; Zhao, Ling; Li, Qi; Mao, Meng; Li, Xuewen; Berglund, Björn

doi:10.1016/j.ijheh.2023.114159

Cited by 8 publications

(3 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The phylogenetic tree showed that the MCR-1 protein of ECSJ33 was also found to be similar with MCR-1.13 isolated from food source in Germany (WP 109545655.1). Given this evidence, we speculated that ECSJ33 may have originated via the food chain and integrated into wastewater ( 8 , 35 , 36 ). This highlights the importance of surveillance efforts to monitor the emergence and dissemination of mcr-1 among the elderly in LTCFs, as well as the co-existence of ESBL genes such as bla TEM-135 and mcr-1 gene carrying E. coli , which have been reported globally, particularly in animal source E. coli isolates from food-producing animal in Poland located on IncX4 plasmid ( 37 ), from human in Thailand located on IncHI/IncN plasmid ( 38 ), and from human in China located on IncI2 plasmid ( 24 ).…”

Section: Discussionmentioning

confidence: 99%

Genetic epidemiology and plasmid-mediated transmission of mcr-1 by Escherichia coli ST155 from wastewater of long-term care facilities

Feng,

Pan,

Zhuang

et al. 2024

Microbiol Spectr

View full text Add to dashboard Cite

One Escherichia coli named ECSJ33 was found from wastewater of a long-term care facility (LTCF) and the plasmid pECSJ33 from ECSJ33 harbored the mobile colistin resistance gene ( mcr-1 ) that was located with 59,080 bp belonging to IncI2 type, which was capable of conjugation with an efficiency of 2.9 × 10 -2 . This paper firstly reports an mcr-1 -carrying E. coli strain ST155 isolated from LTCF in China. Comparative genomics analysis indicated pECSJ33 shared backbone with the previously reported mcr-1 -harboring pHNGDF93 isolated from fish source. The phylogenetic tree revealed MCR-1 protein of ECSJ33 in this study was mostly of animal food origin and that they were closely related. Therefore, the pECSJ33 could be considered as food-origin transmission mcr-1 -harboring plasmid.

show abstract

Section: Discussionmentioning

confidence: 99%

Genetic epidemiology and plasmid-mediated transmission of mcr-1 by Escherichia coli ST155 from wastewater of long-term care facilities

Feng,

Pan,

Zhuang

et al. 2024

Microbiol Spectr

View full text Add to dashboard Cite

show abstract

“…Colistin-resistant E. coli strains have been previously isolated in certain countries, from different sources, including chicken meat, chicken swabs and even wastewater and sludge samples [28,54,55]. For example, Kassem et al, have recently reported the isolation of a highly colistin-resistant E. coli from fresh chicken wings in Lebanon.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Antimicrobial Resistance of Escherichia coli Isolates from Chicken Meat in Romania

Brătfelan,

Tabaran,

Colobatiu

et al. 2023

Animals

View full text Add to dashboard Cite

The current study was conducted in order to analyze the prevalence of Escherichia coli (E. coli) in samples of chicken meat (100 chicken meat samples), as well as to evaluate the antimicrobial susceptibility of these isolates. A total of 30 samples were positive for E. coli among the collected chicken samples. Most isolates proved to be highly resistant to tetracycline (80%), ampicillin (80%), sulfamethoxazole (73.33%), chloramphenicol (70%) and nalidixic acid (60%). Strong resistance to ciprofloxacin (56.66%), trimethoprim (50%), cefotaxime (46.66%), ceftazidime (43.33%) and gentamicin (40%) was also observed. Notably, one E. coli strain also proved to be resistant to colistin. The antimicrobial resistance determinants detected among the E. coli isolates recovered in our study were consistent with their resistance phenotypes. Most of the isolates harbored the tetA (53.33%), tetB (46.66%), blaTEM (36.66%) and sul1 (26.66%) genes, but also aadA1 (23.33%), blaCTX (16.66%), blaOXA (16.66%), qnrA (16.66%) and aac (10%). In conclusion, to the best of our knowledge, this is among the first studies analyzing the prevalence and antimicrobial resistance of E. coli strains isolated from chicken meat in Romania and probably the first study reporting colistin resistance in E. coli isolates recovered from food sources in our country.

show abstract

“…Among the detected microorganisms, Klebsiella pneumoniae is one species exhibiting antibiotic resistance [1]. In addition, Escherichia coli co-harboring conjugative plasmids with colistin-and carbapenem resistance genes have a high prevalence and have been identified from different functional areas of an urban wastewater treatment plant in China [2]. Moreover, the mobile colistin resistance (mcr) genes mcr-8 and mcr-9 were the first reported in Ireland, with the mcr-8-positive Klebsiella pneumoniae displaying resistance to colistin [3].…”

Section: Introductionmentioning

confidence: 99%

The Molecular Identification and Comprehensive Analysis of Klebsiella pneumoniae Isolated from Industrial Wastewater

Yan,

Li,

Wang

et al. 2024

Separations

View full text Add to dashboard Cite

Industrial wastewater typically contains many organic and inorganic pollutants and is also contaminated by various microorganisms. Microbial species in industrial wastewater have not been extensively investigated. In this experiment, a Klebsiella pneumoniae strain was isolated for the first time from industrial wastewater containing a high concentration of sulfate and phosphate. Mass spectrometry, genetic analysis, and biochemical identification were conducted to understand the genetic and biochemical characteristics of this Klebsiella pneumoniae strain recovered from industrial wastewater. Growth experiments revealed that it exhibited an excellent growth rate in nutrient broth. Further analyses showed that the strain was sensitive to most antibiotics but resistant to chloramphenicol and nitrofurantoin. It also exhibited significant resistance to piperacillin/tazobactam and cefotaxime/clavulanic acid. Resistance gene experiments indicated the presence of gyrA, OqxB, and ParC genes associated with antibiotic resistance in the isolated Klebsiella pneumoniae strain. Proteomics uncovered the following three proteins related to drug resistance: the multi-drug resistant outer membrane protein MdtQ, the multi-drug resistant secretion protein, and the modulator of drug activity B, which are coexistent in Klebsiella pneumoniae. Proteomics and bioinformatics analyses further analyzed the protein composition and functional enrichment of Klebsiella pneumoniae. The isolation of Klebsiella pneumoniae from a high concentration in sulfate and phosphate industrial wastewater provides a new direction for further research on the characteristics and drug resistance traits of industrial wastewater microorganisms and the potential risks they may pose when released into the environment.

show abstract

High prevalence of Escherichia coli co-harboring conjugative plasmids with colistin- and carbapenem resistance genes in a wastewater treatment plant in China

Cited by 8 publications

References 55 publications

Genetic epidemiology and plasmid-mediated transmission of mcr-1 by Escherichia coli ST155 from wastewater of long-term care facilities

Genetic epidemiology and plasmid-mediated transmission of mcr-1 by Escherichia coli ST155 from wastewater of long-term care facilities

Prevalence and Antimicrobial Resistance of Escherichia coli Isolates from Chicken Meat in Romania

The Molecular Identification and Comprehensive Analysis of Klebsiella pneumoniae Isolated from Industrial Wastewater

Contact Info

Product

Resources

About