High prevalence of expression of p53 oncoprotein in oral carcinomas from India associated with betel and tobacco chewing

Kuttan, N A; Rosin, Miriam P.; Ambika, K; Priddy, Robert; Bhakthan, N. M. G.; Zhang, L.

doi:10.1016/0964-1955(94)00051-5

Cited by 28 publications

(12 citation statements)

References 22 publications

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“…Two reports (Ranasinghe et al, 1993;Kuttan et al, 1995) have discussed the frequency of p53 staining positivity in oral SCCs in Sri Lanka and India; however, immunohistochemistry is not quantitative, and staining positivity is not always associated with p53 mutation (Hall and Lane, 1994;Baas et al, 1994). For this reason, we performed PCR-SSCP analysis to screen for p53 mutations, and cut and reamplified the abnormal bands to detect p53 mutations by direct sequencing.…”

Section: Mutational Analysesmentioning

confidence: 99%

“…In India and Sri Lanka, some reports have analyzed p53 expression by immunohistochemistry (Kuttan et al, 1995;Ranasinghe et al, 1993;Kaur et al, 1994), however, their frequencies are varied. Moreover, immunohistochemical analysis of the expression of the p53 protein is not quantitative and is not correlated with mutation of the p53 gene (Hall and Lane, 1994;Baas et al, 1994).…”

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confidence: 99%

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Characteristics of mutations in thep53 gene of oral squamous-cell carcinomas associated with betel-quid chewing in Sri Lanka

Chiba

Muthumala²,

Yamazaki

et al. 1998

Int. J. Cancer

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Oral squamous‐cell carcinoma (SCC) is the most common neoplasm in Sri Lanka, accounting for approximately 30% of all cancers in males. Epidemiologic evidence indicates that there is an unequivocal relationship between betel chewing and oral carcinogenesis, suggesting that there may be specific genetic targets of betel‐quid ingredients. The p53 gene has been indicated to be a tumor‐suppressor gene that is found in mutated form in common human cancers; however, there are few reports about “carcinogen‐specific” p53 mutation. Because of this background, primary resected specimens from 23 oral SCCs, 7 leukoplakias and 2 oral submucous fibrosis were collected from oral SCC patients in Sri Lanka and were used for p53 mutation analysis. Exons 5 through 8 of the p53 gene were examined by polymerase chain reaction‐single‐strand conformation polymorphism (PCR‐SSCP) and direct sequencing. Mutations in the p53 gene were frequent (10/23) in oral SCC specimens from Sri Lanka. Moreover, the mutations clustered significantly in exon 5 (7/10) of the p53 gene, and small deletions and inclusions other than point mutations were observed. These results indicate that 1) betel‐quid chewing may cause specific genetic changes, including mutation in the p53 gene; 2) mutations in the p53 gene are not rare events in SCC patients who are betel‐quid chewers, which contrasts with other reports; 3) exon 5 of the p53 gene could be one of the specific targets for some betel‐quid ingredients; and 4) betel‐quid chewing may be a critical environmental factor in the development of oral SCC. Int. J. Cancer 77:839–842, 1998.© 1998 Wiley‐Liss, Inc.

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Section: Mutational Analysesmentioning

confidence: 99%

mentioning

confidence: 99%

Characteristics of mutations in thep53 gene of oral squamous-cell carcinomas associated with betel-quid chewing in Sri Lanka

Chiba

Muthumala²,

Yamazaki

et al. 1998

Int. J. Cancer

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“…However, 27 cases (43.5%) presented with TMD-like symptoms as the first indication of primary and metastatic malignancy. This percentage is higher than in reports of one-third to one-fifth of patients in previous reviews [64][65][66]. Such a proportion indicates that thorough examination is necessary to detect the presence of undisclosed cancers even in patients without any cancer history and that the dentist is in a position to notice unusual symptoms implying the presence of primary malignant lesions.…”

Section: Discussionmentioning

confidence: 61%

Clinical manifestations and diagnostic approach to metastatic cancer of the condyle

Jung

2017

Oral Biol Res

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·············································································································································································Metastatic malignancy on the mandibular condyle is a very rare, but life-threatening condition that is frequently misdiagnosed as temporomandibular joint disorder (TMD) and other dental diseases. The researchers report on a patient who was referred with an initial diagnosis of TMD, due to symptoms resembling TMD, such as limited mouth opening with pain on the temporomandibular joint (TMJ) and mandibular deviation during mouth opening. However, the final diagnosis was malignant condylar metastasis. The researchers reviewed available literature on malignant condylar metastatic cases focusing on initially misdiagnosed cases to find additional diagnostic information. The analysis revealed that some aspects of malignant condylar metastasis were overlooked, which frequently led to misdiagnosis and incorrect treatment. Further understanding of condylar metastasis might lead to early detection of occult primary cancer, possibly preventing further metastasis.

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“…[27] Kuttan observed that 13 of the 23 OSCCs (56.5%) demonstrated nuclear p53 staining. [28] In the study conducted by Danieli, they found p53 immunoexpression in 44% of tumours from patients with tongue SCC. [10] Atula observed p53 protein expression in 33.3% tongue SCC samples.…”

Section: Discussionmentioning

confidence: 97%

Oral squamous cell carcinomas in age distinct population: A comparison of p53 immunoexpression

Chandra

Singh

Sebastian³

et al. 2013

J Can Res Ther

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High prevalence of expression of p53 oncoprotein in oral carcinomas from India associated with betel and tobacco chewing

Cited by 28 publications

References 22 publications

Characteristics of mutations in thep53 gene of oral squamous-cell carcinomas associated with betel-quid chewing in Sri Lanka

Characteristics of mutations in thep53 gene of oral squamous-cell carcinomas associated with betel-quid chewing in Sri Lanka

Clinical manifestations and diagnostic approach to metastatic cancer of the condyle

Oral squamous cell carcinomas in age distinct population: A comparison of p53 immunoexpression

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