High Prevalence of Leptotrichia amnionii, Atopobium vaginae, Sneathia sanguinegens, and Factor 1 Microbes and Association of Spontaneous Abortion among Korean Women

Abstract

Objective. The purpose of this study was to (i) determine the cervical microbial composition in different abortion samples and to (ii) investigate the correlation between spontaneous abortion and cervical microbes in Korean women. Methods. We collected cervical swabs from women who had never undergone abortion (N = 36), had spontaneous abortion (N = 23), and had undergone induced abortion (N = 88) and subjected those samples to 16S rRNA pyrosequencing. Further, factor analysis and correlation between cervical microbiota and spontaneous abortion were evaluated by logistic regression analysis. Results. In spontaneous abortion women, 16 S rRNA gene sequences showed significant increases in Atopobium vaginae, Megasphaera spp., Gardnerella vaginalis, Leptotrichia amnionii, and Sneathia sanguinegens compared to women in nonabortion group. In multivariate logistic regression analysis, A. vaginae (OD = 11.27; 95% = 1.57–81), L. amnionii (OD = 11.47; 95% = 1.22–107.94), S. sanguinegens (OD = 6.89; 95% = 1.07–44.33), and factor 1 microbes (OD = 16.4; 95% = 1.88–42.5) were strongly associated with spontaneous abortion. Conclusions. This study showed a high prevalence of L. amnionii, A. vaginae, S. sanguinegens, and factor 1 microbes in spontaneous abortion and association with spontaneous abortion in Korean women.

The Vaginal Microbial Signatures of Preterm Birth Delivery in Indian Women

“…It was previously shown that G. vaginalis acts as a preterm signature in European ancestry ( Callahan et al., 2017 ). Although, similar reports from other cohorts are available ( Romero et al., 2014 ; Seo et al., 2017 ; Tabatabaei et al., 2019 ), the genomic content of these abundant bacterial species from the same cohorts have not been explored. For a better and more profound understanding, how the presence or absence of a bacterial species effect the composition of a microbial ecosystem or host physiology, it is important to decode their genome and identify the pertinent functions.…”

Revealing the Disturbed Vaginal Micobiota Caused by Cervical Cancer Using High-Throughput Sequencing Technology

“…Sneathia spp. is a gram-negative anaerobic bacterium, and previous studies have reported that the increase in abundance of Sneathia is related to the occurrence of spontaneous abortion ( Seo et al., 2017 ), non-gonococcal urethritis ( Manhart et al., 2013 ) and bacterial vaginosis ( Millar, 2017 ). In addition, previous studies have confirmed that the relative abundance of Sneathia genus is elevated to varying degrees in the vaginal microbiota of patients with cervical precancerous lesions ( Łaniewski et al., 2018 ).…”

The Microbiome and Preterm Birth: A Change in Paradigm with Profound Implications for Pathophysiologic Concepts and Novel Therapeutic Strategies

“…Microbiomes change during pregnancy, and differences in composition have long been acknowledged to account for variations in preterm birth rates [ 11 , 16 , 40 , 41 ]. The reduction of microbial richness and diversity and changes in microbiome structure seem to occur early, in the first to second trimester of pregnancy, and there seem to exist racial disparities [ 14 , 42 – 44 ]. That is, the cervical microbiota from women with Chlamydia trachomatis infection, which predisposes for preterm birth, differ completely from that of healthy women with respect to microbial diversity.…”