High prevalence of increased trisialotransferrin concentrations in patients with anorexia nervosa: Implications for determination of carbohydrate-deficient transferrin

The effect of malnutrition on protein glycosylation in childhood

“…Thus, it is suggested that elevated CDT values from the past studies using immunoassay method are most likely due to an incomplete separation of trisialotransferrin from CDT on the previous fractionation step and thus overdetermination of CDT. On the other hand, these results indicate that trisialotransferrin fraction may be used as a laboratory marker of (hypocaloric) malnutrition in this study [ 19 ] .…”

“…In a recent study by Arndt et al, CDT was analyzed in AN patients by three different methods including high performance liquid chromatography (HPLC), capillary electrophoresis (CE) and immunoassay. However, in contrast to the immunoassay tests, no elevation of CDT in anorexia nervosa patients was detected by HPLC and CE [ 19 ] . An interesting finding of this study was increased trisialotransferrin isoform that was detected by HPLC in 67% of AN patients.…”

“…There are only few studies that investigated the effect of catabolic state on glycosylation. In these studies, CDT has been detected by different methods [ 17 - 19 ] . The analysis of all Tf isoforms separately was performed in only one of these studies [ 19 ] .…”

“…In these studies, CDT has been detected by different methods [ 17 - 19 ] . The analysis of all Tf isoforms separately was performed in only one of these studies [ 19 ] . To our knowledge, there is no study investigating the relationship between protein energy malnutrition and transferrin isoforms in children in the literature.…”

First evaluation of a multi-capillary electrophoresis CDT assay on Helena Biosciences' V8 analyser

“…However, it is important to recognize other clinical conditions that can lead to an abnormal Tf glycosylation pattern including polymorphisms within the Tf gene [4,5], rare hereditable deficiency in the glycosylation enzymes [6][7][8], hereditary fructose intolerance and galactosemia [9] and some other clinical conditions that are apparently unrelated to excessive alcohol consumption [10][11][12].…”