High Prevalence of Iron Acquisition Genes Among <i>Acinetobacter baumannii</i> Strains Isolated From Patients With Urinary Tract Infections in Southeast of Iran

Abdi, Hussein Ali; Hormozi, Bahman; Najimi, Mohsen; Noorzehi, Nafiseh

doi:10.17795/ajcmi-30656

Cited by 2 publications

(5 citation statements)

References 12 publications

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“…However, this frequency has not previously been described. The levels observed in this investigation are far below those observed by Abdi et al [ 3 ]. They reported that frequencies of tonB , barA , feoB , entA , and hemO genes were 85%, 97%, 99%, 99%, and 95%, respectively.…”

Section: Discussioncontrasting

confidence: 86%

“…According to Abdi et al [ 3 ], the PCR method was used for the detection of the IUS gene. For partial gene, the PCR conditions used to amplify the iron/siderophore mediate gene were the same as described in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, PCR was performed using a C1001 Bio-Rad thermal cycler. Finally, 5 μ l of amplified product is electrophoresed on 1.5% agarose gel at a constant of 80 V for 60 min [ 3 ].…”

Section: Methodsmentioning

confidence: 99%

“…It has become more challenging to treat A. baumannii infections in the hospitalize portions because of their resistance to significant groups of antimicrobial agents such as β -lactams [ 2 ]. Various resistance mechanisms make A. baumannii a successful pathogen of the 21 st century [ 3 ]. Extended-spectrum β -lactamases (ESBL) belong to the class A β -lactamases.…”

Section: Introductionmentioning

confidence: 99%

“…However, A. baumannii synthesizes siderophores which are relatively low-molecular-weight agents capable of converting polymeric ferric oxy-hydroxides to soluble iron chelates under low iron stress. The ability of bacteria to assimilate iron is related to invasiveness [ 3 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

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A Comprehensive Study of the Relationship between the Production of β-Lactamase Enzymes and Iron/Siderophore Uptake Regulatory Genes in Clinical Isolates of Acinetobacter baumannii

Porbaran

Tahmasebi

Arabestani

2021

International Journal of Microbiology

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Background.The iron/siderophore uptake system (IUS) involved in the Acinetobacter baumannii pathogenicity. However, IUS’s role in antibiotic resistance and the production of β-lactamase enzymes of A. baumannii are unclear. This study aimed to investigate the relationship between the production of β-lactamase enzymes and IUS regulatory genes in clinical isolates of A. baumannii. Methods. A. baumannii isolates were collected from clinical isolates using biochemical tests. The antibiotic resistance patterns and β-lactamase-producing strains were identified using the disk diffusion method (DDM). Also, IUS genes were detected by the polymerase chain reaction (PCR) method. Results. Seventy-two (72) A. baumannii isolates were collected from a different clinical specimen. Gentamicin-resistant strains (43%) had the highest frequency, and aztreonam-resistant strains (12.5%) had the lowest frequency. Also, the distribution of AmpC and MBL producing isolates were 27.7% and 35%, respectively. Moreover, the frequencies of basD, bauA, pld, paaE, entA, feoB, hemO, and tonB genes were as follows: 12.5%, 15.2%, 11.1%, 15.2%, 19.4%, 16.6%, 23.6%, and 6.9%. Further, a strong correlation was observed between the abundance of β-lactamase-producing strains and IUS genes. Conclusions. Based on our knowledge from this study, the association between β-lactamase production and IUS genes in A. baumannii plays an essential role in the emergence of drug-resistant strains.

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Section: Discussioncontrasting

confidence: 86%

Section: Methodsmentioning

confidence: 99%

“…Moreover, PCR was performed using a C1001 Bio-Rad thermal cycler. Finally, 5 μ l of amplified product is electrophoresed on 1.5% agarose gel at a constant of 80 V for 60 min [ 3 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Comprehensive Study of the Relationship between the Production of β-Lactamase Enzymes and Iron/Siderophore Uptake Regulatory Genes in Clinical Isolates of Acinetobacter baumannii

Porbaran

Tahmasebi

Arabestani

2021

International Journal of Microbiology

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Prevalence of the per, tem, veb, shv genes in Acinetobacter baumannii Isolated from Educational Hospital of Zahedan, Iran

Ordoni

Amini

Ranayi

et al. 2022

Iran J Med Microbiol

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Background and Aim: Acinetobacter baumannii is one of the most important pathogens found in ICUs as it is responsible for nosocomial infection with a high mortality rate and a rising level of resistance to most antibiotics. One of the main mechanisms of resistance is the production of extended-spectrum beta-lactamase (ESBL) genes such as veb, per, tem, and shv. The current study aimed to determine the antibiotic resistance pattern and the frequency of per, tem, veb, and shv genes in A. baumannii strains isolated from Zahedan, Iran. Materials and Methods:The current study was conducted on 150 strains of A. baumannii isolated from different clinical samples from January to September 2018. The antibiotic resistance pattern was determined by the disk diffusion method for 18 antibiotics and the minimum inhibitory concentration for colistin. Later, the bacterial genome was extracted, and PCR detected ESBL genes.Results: Out of the 150 studied isolates, 141 were A. baumannii and only nine isolates were A. nosocomialis. A. baumannii strains were strongly resistant to many selected antibiotics such as CAZ (99.3%), CTX (97.9), PTZ (97.2%), SXT (97.2%), IMI (97.2%), and LEV (96.5%); while this value was low for a few antibiotics including MN (69.5%), DXT (52.5%), SAM (21.3), and TN (16.7). The eb, per, tem, and shv genes were detected in 20 (13.3%), 15 (10%), 23 (15.4%), and 11 (7.3%) isolates, respectively. Conclusion:The isolates were highly resistant to ceftazidime, ceftriaxone, piperacillin-tazobactam, cotrimoxazole, imipenem, and levofloxacin; in addition, the frequency of per, veb, tem, and genes was lower in the current study than those reported in other regions.

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High Prevalence of Iron Acquisition Genes Among Acinetobacter baumannii Strains Isolated From Patients With Urinary Tract Infections in Southeast of Iran

Cited by 2 publications

References 12 publications

A Comprehensive Study of the Relationship between the Production of β-Lactamase Enzymes and Iron/Siderophore Uptake Regulatory Genes in Clinical Isolates of Acinetobacter baumannii

A Comprehensive Study of the Relationship between the Production of β-Lactamase Enzymes and Iron/Siderophore Uptake Regulatory Genes in Clinical Isolates of Acinetobacter baumannii

Prevalence of the per, tem, veb, shv genes in Acinetobacter baumannii Isolated from Educational Hospital of Zahedan, Iran

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