High Prevalence of Multidrug-Resistant Bacteria in the Centre Hospitalier et Universitaire de la Mère et de l’Enfant Lagune (CHU-MEL) Reveals Implications of Poor Hygiene Practices in Healthcare

Dougnon, Victorien; Koudokpon, Hornel; Hounmanou, Yaovi Mahuton Gildas; Azonbakin, Simon; Fabiyi, Kafayath; Oussou, Alida; Codjia, Ida; Ahoyo, Angèle; Baba-Moussa, Lamine; Bankolè, Honoré

doi:10.1007/s42399-019-00149-3

Cited by 8 publications

(13 citation statements)

References 27 publications

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“…The comparison of the resistance profiles showed that there were the same profiles of circulating strains in the environment and wounds. The resistance proportions obtained for bacteria isolated from wounds and the environment in the present study are close to those of bacteria isolated from wounds and the environment at the University Hospital of Mother and Child-Lagoon (16). This study, therefore, confirms the role of the environment, especially the collection rooms, in the colonization of wounds in patients.…”

Section: Discussionsupporting

confidence: 85%

“…For Gram-negative bacilli, the performed tests were oxidase test, glucose fermentation test, and realization of the API 20E gallery for Enterobacteriaceae differentiation. Biochemical tests for the identification of Grampositive cohorts were catalase, free staphylocoagulase, and DNase production (16,18).…”

Section: Bacteriological Examinationmentioning

confidence: 99%

“…The inhibition zone diameters were compared to the inhibition diameters proposed by the CA-SFM (20). The choice of antibiotic discs was inspired by the recommendations of the French Society for Microbiology and conformed to those used by Dougnon et al (16).…”

Section: Antibiotic Susceptibility Testmentioning

confidence: 99%

“…These studies have shown poor knowledge and poor application of standard precautions in environments heavily contaminated with multidrug-resistant bacteria. A study by Dougnon et al (16) showed the contamination of the operative wounds of women with cesarean sections by multidrug-resistant bacteria coming from the environment of the sampling room of the University Hospital of the Mother and Child-Lagoon of Cotonou. The involvement of invasive procedures in the occurrence of bacteremia linked to catheters was also noted by this study.…”

Section: Introductionmentioning

confidence: 99%

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Infection Risks and Antimicrobial Resistance in Tertiary Hospitals in Benin: Study Cases of Sakété-Ifangni and Menontin Hospitals

Dougnon

Koudokpon

Chabi³

et al. 2020

Int J Infect

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Background: Antimicrobial resistance surveillance and infection risk assessment are important for optimizing the quality of healthcare in hospitals. Objectives: This study aimed to evaluate the antimicrobial resistance and infection risks in Tertiary hospitals in Benin. Methods: Bacteriological examinations were carried out on swab samples from the hospital environment at Sakété-Ifangni Hospital and Menontin Hospital in Benin. The environmental swabs were supplemented with wound swabs from Menontin Hospital. In both hospitals, antibiotic susceptibility of the isolated strains was determined by the agar diffusion method. Results: The results showed that coagulase-negative staphylococci (45%) comprised the most isolated species in the environment of the Surgery and Operating Departments of Sakété-Ifangni Hospital, followed by Staphylococcus aureus (27.50%) and Proteus spp. (15%). At Menontin Hospital, only one specimen was sterile from wound swabs. From environmental swabs, 29 out of 45 samples were positive. Staphylococcus aureus (31%), Escherichia coli (15%), and coagulase-negative staphylococci (12%) were the most isolated species from wounds. Strains of coagulase-negative staphylococci (29%), Pseudomonas aeruginosa (15%), and Proteus mirabilis (12%) were the most common strains in swabs. The study of the adequacy of treatment of infections caused by bacteria responsible for wound suppuration showed that 52% of the wounded received appropriate antibiotic treatment. At Menontin Hospital, many isolated strains from room environment samples were found in the wounds of the patients. In both hospitals, the isolated strains were multiresistant to conventional antibiotics. Conclusions: These data show how surveillance of infection risks and antimicrobial resistance is important to prevent healthcareassociated infections.

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Section: Discussionsupporting

confidence: 85%

Section: Bacteriological Examinationmentioning

confidence: 99%

Section: Antibiotic Susceptibility Testmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Infection Risks and Antimicrobial Resistance in Tertiary Hospitals in Benin: Study Cases of Sakété-Ifangni and Menontin Hospitals

Dougnon

Koudokpon

Chabi³

et al. 2020

Int J Infect

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“…According to Douard [26], it is therefore when the catheter is placed that the bacteria are inserted. A second factor linked to the occurrence of bacteremia is the quality of disinfection [27]. This includes hand hygiene, the quality of the antiseptic used for hand hygiene and disinfection of the operating site [28].…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Enterobacteriaceae producing β-lactamase and methicillin-resistant staphylococci isolated from the hospital environment and catheters in two public hospitals in Benin

Gbotche¹,

Dougnon²,

Chabi³

et al. 2020

Preprint

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Background: Antimicrobial resistance is a real public health problem. All over the world, it has a considerable impact in hospitals. The present study aims to analyze the presence of resistance genes and bacterial ecology in two hospitals in Benin. Methods: To do this, 146 environmental and catheter samples were collected at the University Hospital Center of Abomey-Calavi / So-Ava and at the Beninese Army Hospital. These samples were inoculated on Mannitol Salt and Eosin Methylene Blue agars. The colonies obtained were identified and their sensitivity to antibiotics was tested, using the usual bacteriological techniques. Four resistance genes encoding the production of extended spectrum beta-lactamases (blaCTX-M1, blaCTX-M2, blaCTX-M9, blaCTX-M15) have been searched in the genome of enterobacteriaceae strains. At the level of staphylococci, the gene coding for methicillin resistance (mecA) was sought.Results: At the end of this study, 69 strains of enterobacteria and 60 of staphylococci were identified. A predominance of Staphylococcus aureus (25.6%) followed by Enterobacter cloacae (21.0%) and coagulase negative staphylococci (21.0%) was noted. These bacterial strains showed to be multidrug-resistant, particularly to beta-lactams, fluoroquinolones, aminoglycosides and macrolides. Beta-lactamases were identified in the genome of bacterial strains with a predominance of blaCTX-M15 (42.8%). The frequency of the mecA gene in staphylococci was 50%.Conclusions: These results show the magnitude of the antimicrobial resistance situation in hospitals. They can be used to support advocacy for urgent action at the national level, especially with regard to the management and efficient use of antimicrobials in Benin.

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Hospital effluents as sources of antibiotics residues, resistant bacteria and heavy metals in Benin

Déguénon

Dougnon

Houssou³

et al. 2022

SN Appl. Sci.

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This study aims to evaluate the bacteriological, chemical, and toxicological quality of hospital effluents in Benin. Eighteen (18) samples were collected from the south and north of Benin, and poles were set up. Bacteriological analysis was carried out according to the French National Organization for Standardization (AFNOR) standard. Identification of the bacterial species isolated was performed using the API 20E gallery and specific biochemical tests. Antibiotic residues were searched by following ELISA kit instructions. 109 bacterial isolates were obtained, with a predominance of non-enterobacteria (37.7%) and Acinetobacter spp. (24.6%), followed by Klebsiella spp. (11.48%); S. aureus and Coagulase-negative staphylococci (CoNS) were isolated at the same frequency (6.5%). Physico-chemical and toxicological parameter analyses showed that they were in conformity with the standards of discharge into the environment. The pH, temperature, electrical conductivity, total solids below, P-redox and suspended solids measured were, on average, 6.83, 27.21 °C, 693.68 µS/cm, 693.68 mg/l, 1.68 mV and 0.15 mg/l, respectively. For heavy metals, cadmium was most prevalent (0.22 mg/l), followed by nickel (0.18 mg/l) and lead (0.03 mg/l). The average antibiotic residue concentrations ranged from 0.043 to 7.65 µg/l. Ciprofloxacin, metronidazole and sulfamethoxazole had the highest residue concentrations of 7.65 ± 2.272 µg/l, 6.61 ± 0.051 µg/l and 3.88 ± 3.088 µg/l, respectively. The heavy metal concentrations obtained were below those required by Benin standards. These effluents therefore present health and environmental risks. It is then more than necessary to develop treatment methods for them before their rejection into the natural environment.

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High Prevalence of Multidrug-Resistant Bacteria in the Centre Hospitalier et Universitaire de la Mère et de l’Enfant Lagune (CHU-MEL) Reveals Implications of Poor Hygiene Practices in Healthcare

Cited by 8 publications

References 27 publications

Infection Risks and Antimicrobial Resistance in Tertiary Hospitals in Benin: Study Cases of Sakété-Ifangni and Menontin Hospitals

Infection Risks and Antimicrobial Resistance in Tertiary Hospitals in Benin: Study Cases of Sakété-Ifangni and Menontin Hospitals

Molecular characterization of Enterobacteriaceae producing β-lactamase and methicillin-resistant staphylococci isolated from the hospital environment and catheters in two public hospitals in Benin

Hospital effluents as sources of antibiotics residues, resistant bacteria and heavy metals in Benin

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