High Prevalence of Multiple Strain Colonization of Helicobacter pylori in Korean Patients: DNA Diversity Among Clinical Isolates from the Gastric Corpus, Antrum and Duodenum

Kim, Jeong Wook; Kim, Jae Gyu; Chae, Seok Lae; Joο, Young Eun; Park, Sill Moo

doi:10.3904/kjim.2004.19.1.1

Cited by 17 publications

(12 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, further studies are necessary to determine whether size variation of the promoter region affects transcriptional activity of galT . Additionally, two strains showed multiple bands, indicating a mixed population of cells within the isolate, suggesting that the patient was colonized with multiple H. pylori strains or a single strain with clonal variants, phenomena that have been observed previously [43,52–55]. Alternatively, these multiple bands could be the result of intragenomic or intergenomic recombination, commonly observed in H. pylori at various loci [35,56–58], including between Le synthesis genes [44].…”

Section: Discussionmentioning

confidence: 71%

Genotypic and Phenotypic Variation of Lewis Antigen Expression in Geographically Diverse Helicobacter pylori Isolates

et al. 2011

View full text Add to dashboard Cite

Background Helicobacter pylori is a persistent colonizer of the human gastric mucosa, which can lead to the development peptic ulcer disease and gastric adenocarcinomas. However, H. pylori can asymptomatically colonize a host for years. One factor that has been hypothesized to contribute to such persistence is the production of Lewis (Le) antigens in the lipopolysaccharide layer of the bacterial outer membrane as a form of molecular mimicry, since humans also express these antigens on their gastric mucosa. Humans and H. pylori both are polymorphic for Le expression, which is driven in H. pylori by variation at the Le synthesis loci. In this report we sought to characterize Le genotypic and phenotypic variation in geographically diverse H. pylori isolates. Materials and Methods From patients undergoing endoscopy in 29 countries, we determined Le phenotypes of 78 H. pylori strains, and performed genotyping of the galT and β-(1,3)galT loci in 113 H. pylori strains. Results Le antigen phenotyping revealed a significant (p <0.0001) association between type 1 (Lea and Leb) expression and strains of East-Asian origin. Genotyping revealed a significant correlation between strain origin and the size of the promoter region upstream of the Le synthesis gene, galT (p <0.0001). Conclusion These results indicate that the heterogeneity of human Le phenotypes are reflected in their H. pylori colonizing strains, and suggest new loci that can be studied to assess variation of Le expression.

show abstract

Section: Discussionmentioning

confidence: 71%

Genotypic and Phenotypic Variation of Lewis Antigen Expression in Geographically Diverse Helicobacter pylori Isolates

et al. 2011

View full text Add to dashboard Cite

show abstract

“…In a study by Finger et al in Peru, at least 50z of patients showed infection with 2 H. pylori strains, however 61z of these patients were infected with closely related strains (19). In other studies in Korea and India, it was shown that 67.5z and 100z of the studied patients harbored multiple H. pylori strains, respectively (21,22).…”

Section: Discussionmentioning

confidence: 99%

Genotype Diversity and Quasispecies Development of <i>Helicobacter pylori</i> in a Single Host

et al. 2015

View full text Add to dashboard Cite

SUMMARY:Infection with different strains of Helocobacter pylori and emergence of new genomic variants during their long-term gastric colonization are assumed to be the main reasons for eradication failure. We used genotyping and arbitrarily primed PCR fingerprinting (RAPD) to detect relatedness and genetic variations among H. pylori single isolates from each patient in Iran. Multiplex-PCR amplification of gene alleles encoding the virulence factors vacA (m/s), cagA, and iceA (A1/A2) and comparison of RAPD patterns of different singles colonies were performed for each individual patient's isolate. Results showed a high frequency of diversity among the H. pylori strains. Nearly 23z of infected patients showed a single type infection. The infection types related, unrelated, and related/unrelated were found among 25.6z, 12.8z, and 38.5z of patients, respectively. Both mixed type infections (77z) and quasispecies development (15.4z) were detected in these patients. Genotype conversion among vacA (41.6z), cagA (41.6z), and iceA (50z) alleles was observed for the strains with identical or related RAPD patterns. Coevolution of different alleles was also detected in a patient infected with strains presenting the same RAPD patterns. Collectively, results of this study revealed the occurrence of quasispecies development, mixed type infections, and changes in virulence properties of H. pylori strains among the studied patients.

show abstract

“…Stomachs can be colonised by multiple strains (Kibria et al 2015; J. W. Kim et al 2004;Ben Mansour et al 2016). Some of the strains could have been linked to gastric cancer, when they were actually not the strains driving the carcinogenesis.…”

Section: Clonalframe Based Methods Although Well-adapted For Other Bmentioning

confidence: 99%

“…A good example of diversity can be seen by the fact that two strains of H. pylori found in different patients (or even in patients from a same family) are often extremely different (Kivi et al 2007). Different strains of H. pylori can also colonise a same host (Kibria et al 2015; J. W. Kim et al 2004;Ben Mansour et al 2016). The H. pylori genome is incredibly diverse, with variation in genome size (Dong et al 2014), gene presence, gene order, and allelic profile (Thorell et al 2016).…”

Section: H Pylori Genome Variabilitymentioning

confidence: 99%

“…This chapter focuses on bacterial genomics factors, but host and environmental factors will be used to explain the variations found in the bacteria genome. Another element to keep in mind is the fact that in the event of a H. pylori infection, the stomach is not colonised by a unique strain, but a mix of strains, sometimes closely related, but sometimes resulting from a mixed infection (Kibria et al 2015; J. W. Kim et al 2004;Ben Mansour et al 2016). Therefore linking bacterial genotypes with gastric cancer is not straightforward.…”

Section: A Genome Wide Association Study Of Helicobacter Pylori In Camentioning

confidence: 99%

See 1 more Smart Citation

Genome evolution and virulence in H. pylori: Identifying the genes/alleles underlying phenotype variation

Berthenet¹

View full text Add to dashboard Cite

An estimated 50% of all people carry the stomach bacterium Helicobacter pylori (H. pylori). This organism is responsible for gastric problems like gastritis and gastric ulcers, and is one of the major causes of gastric cancer worldwide. Large numbers of people carry this organism asymptomatically and many questions remain about why serious symptoms develop in a subset of infected humans. These extremely recombinant bacteria may take different evolutionary trajectories in different people, and some genomic changes may be associated with gastric cancer. To test this, and learn more about the genetics of cancer-associated H. pylori, different approaches were used. First, evolution of H. pylori populations was investigated looking at both core and accessory genomes and revealed traces of the long and complex history of the Americas in the bacterial genomes, as well as a similar evolution in core and accessory genome. This was the first time accessory genome of H. pylori was studied that way. Secondly, evolution occurring in the bacterial genome during colonisation of a single host was studied in mice model. This analysis revealed small changes during the passage from a human host to a mice host, and during the long-term colonisation of mice stomach. Then a Genome Wide Association Study (GWAS) approach was applied to a large isolate collection sampled across Europe comprising strains isolated from cancer patients and strains from asymptomatic or gastritissuffering patients. This approach identified 11 polymorphisms in 9 genes (3 cagPAI genes, babA, hpaA, 1 outer membrane protein coding gene HP1055 and 3 other core genes (HP0747, HP0709 and HP0468) associated with cancer and a preliminary risk score was built to identify high risk strains. Finally, variations observed among clinical isolates of H. pylori from European patients with different pathologies in terms of motility and ability to trigger cytokine production in two types of cells were quantified. Motility variations were not associated with the disease type, but a link was observed for cytokine production. This was compared to genomic variations, confirming the role of known genomic factors such as cagPAI genes and sheding light to possible functions of a number of new genes.

show abstract

High Prevalence of Multiple Strain Colonization of Helicobacter pylori in Korean Patients: DNA Diversity Among Clinical Isolates from the Gastric Corpus, Antrum and Duodenum

Cited by 17 publications

References 35 publications

Genotypic and Phenotypic Variation of Lewis Antigen Expression in Geographically Diverse Helicobacter pylori Isolates

Genotypic and Phenotypic Variation of Lewis Antigen Expression in Geographically Diverse Helicobacter pylori Isolates

Genotype Diversity and Quasispecies Development of <i>Helicobacter pylori</i> in a Single Host

Genome evolution and virulence in H. pylori: Identifying the genes/alleles underlying phenotype variation

Contact Info

Product

Resources

About