High Prevalence of Pantoea in Diaphorina citri (Hemiptera: Liviidae): Vector of Citrus Huanglongbing Disease

Güz, Nurper; Arshad, Muhammad; Çağatay, Naciye Sena; Dageri, Asli

doi:10.1007/s00284-020-01969-6

Cited by 2 publications

(2 citation statements)

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“…Total genomic DNA was isolated individually from 164 specimens, including 19 adults and 146 larvae, using a modified cetyltrimethylammonium bromide protocol (CTAB) with minor modifications (Doyle and Doyle, 1987;Guz et al, 2020a). Briefly, each specimen was homogenized in lysis buffer (100mM Tris, 50mM EDTA, 1.4M NaCl, 2% CTAB) and incubated at 65 °C for 12 h. After chloroformisoamyl alcohol (24:1) extraction, the DNA samples were precipitated using isopropanol.…”

Section: Methodsmentioning

confidence: 99%

“…Pantoeais another common bacterium with more than 20 species that have adapted to diverse ecological niches, including humans, insects, plant material, soil, and water (Walterson and Stavrinides, 2015). Recent studies showed that Pantoea species contribute to providing essential nutrients limited in the host's diet (Kenyon et al, 2015), participating in creating a nitrogen-rich environment for developing eggs and larvae (MacCollom et al, 2009) and breakdown of plant cell-wall biopolymer (Bozorov et al, 2019).Pantoea isolates have been reported from different insect species, such as fruit fly (Lauzon et al 2009) and Asian citrus psyllid (Guz et al 2020b).…”

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confidence: 99%

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Analysis of genetic variation in an important pest, Tutaabsoluta, and its microbiota with a new bacterial endosymbiont

2021

Turk J Agric For

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Tomato leaf miner is an aggressive and invasive pest that causes serious damage in tomato production resulting in up to 100% yield losses during serious outbreaks. The management of tomato leaf miner with synthetic pesticides is difficult due to its leaf mining habit, short life span, high reproductive rates and inseciticide resistance problem. In this study, sequences of the mitochondrial cytochrome oxidase I (mtCOI) gene of Tuta absoluta 2 specimens were analyzed and compared with the available COI sequence data members from GenBank. All specimens of tomato leaf miner were clustered together in a single group with a high support value, suggesting the presence of a homogeneity in the T. absoluta specimens. Furthermore, 164 samples were analyzed in order to detect the prevalence of Arsenophonus, Cardinium, Hamiltonella, Pantoea, Spiroplasma and Wolbachia infection in T. absoluta populations. PCR analysis revealed 100% prevalence Wolbachia and Pantoea however, no other symbiotic bacterial infections were observed in T. absoluta populations. Finally, Wolbachia wsp, Pantoea 16S ribosomal RNA genes and mtDNA COI haplotypes were analyzed in order to estimate the mitochondrial effects of these endosymbionts on the population structure of tomato leaf miner. The findings of high genetic homogeneity among T. absoluta populations with high prevalence of Wolbachia and Pantoea infections seems to imply that both bacterial infections has less impact on the mtDNA variations.

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Section: Methodsmentioning

confidence: 99%