High Prevalence of Rotavirus G4P[6] Genotypes among Neonates in Two Korean Hospitals

Kim, Jae‐Seok; Kim, Sung Mi; Kim, Hyun Soo

doi:10.5145/acm.2017.20.3.63

Cited by 7 publications

(9 citation statements)

References 14 publications

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“…This genotype had not been reported previously in Korea. Since several studies have reported that most neonatal rotavirus infections in Korea are caused by G4P[6] strains [ 5 – 9 ], G8P[6] emergence in neonates is remarkable and worth reporting. The G8 genotype was first detected in humans in Indonesia in 1980, and was sporadically detected worldwide, especially in Africa [ 12 – 15 ].…”

Section: Discussionmentioning

confidence: 99%

“…In Korea, G1P[8] is the most frequent genotype in children, and G4P[6] is the most frequent genotype in neonates [ 3 , 5 – 8 ]. The predominance of the G4P[6] genotype in Korean neonates has been frequently reported in several studies from 1999 to 2016 [ 5 – 9 ]. Interestingly, G4P[6] predominance was not reported in other countries.…”

Section: Introductionmentioning

confidence: 99%

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Emergence of G8P[6] rotavirus strains in Korean neonates

et al. 2018

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BackgroundRotaviruses are the major causes of pediatric gastroenteritis worldwide. The genotypic distribution of rotavirus strains shows temporal and geographical fluctuations, and knowledge of the molecular epidemiology of rotaviruses is important for the development of vaccines and diagnostic reagents. We investigated VP4 and VP7 capsid genotypes of rotaviruses isolated from 211 stool specimens collected from Korean neonates in a neonatal intensive care unit from September 2017 to March 2018.ResultsOf 211 stool specimens, 15 specimens (7.1%) were rotavirus-positive. Eleven specimens (73.3%) were G8P[6] type and 4 (26.7%) were G4P[6] type. Sequence analysis revealed that all G8 sequences in this study showed the highest nucleotide identity to G8 sequences of G8P[8] rotavirus strains isolated in Vietnam in 2014, and P[6] gene sequences showed the highest nucleotide identity to P[6] sequences of G4P[6] strains detected in Korea in 2012. Only one amino acid difference in VP7 was found in 3 of the 11 G8P[6] strains in this study, but multiple amino acid substitutions in VP7 were detected between these G8P[6] strains and the commonly used vaccine strains.ConclusionsThis study showed that rotavirus G8P[6] strains were firstly detected at high frequency in Korean neonates from September 2017 to March 2018. These new rotavirus G8P[6] strains were estimated to be derived from reassortment events between the G8 of G8P[8] strains in Asian region and the P[6] of G4[6] in Korea. Whether the emergence of this unusual G8P[6] strain reflects continuous prevalence or transient occurrence will require continuous monitoring of rotavirus epidemiology.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Emergence of G8P[6] rotavirus strains in Korean neonates

et al. 2018

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“…In this study, we performed whole genome sequencing of 20 rotavirus G4P[6] strains isolated from Korean neonates, and all 20 G4P[6] strains showed G4-P[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1, a Wa-like genotype constellation. The G4P[6] strain has been frequently isolated in Korean neonates since 1999, but is rare in other countries [9–13]. Rotavirus G4P[6]/G4P[x]/GxP[6] genotypes were identified in 100% of neonatal cases at a hospital in Guri, Korea (2001–2003), 100% at a hospital in Busan (2013), 92.6% at a hospital in Seoul (2011–2012), and 93.7% of neonatal cases at a hospital in Hwaseong, Korea (2013) [9–13].…”

Section: Discussionmentioning

confidence: 99%

“…The G4P[6] strain has been frequently isolated in Korean neonates since 1999, but is rare in other countries [9–13]. Rotavirus G4P[6]/G4P[x]/GxP[6] genotypes were identified in 100% of neonatal cases at a hospital in Guri, Korea (2001–2003), 100% at a hospital in Busan (2013), 92.6% at a hospital in Seoul (2011–2012), and 93.7% of neonatal cases at a hospital in Hwaseong, Korea (2013) [9–13]. Because only the VP7 and VP4 genes of G4P[6] strains have been analyzed previously, this is the first study to analyze whole-genome sequences of all 11 genes of G4P[6] strains detected in Korea, particularly in Korean neonates.…”

Section: Discussionmentioning

confidence: 99%

“…In Korea, G1P[8] is the most frequent genotype in children, and G4P[6] is the most frequent genotype in neonates [3, 9–12]. The predominance of the G4P[6] genotype in Korean neonates has been frequently reported in several studies from 1999 to 2016 [9–13]. Interestingly, the predominance of G4P[6] in neonates has not been reported in other countries.…”

Section: Introductionmentioning

confidence: 99%

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Whole-genome analysis of rotavirus G4P[6] strains isolated from Korean neonates: association of Korean neonates and rotavirus P[6] genotypes

et al. 2019

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Background Group A rotaviruses are the major causative agents of pediatric gastroenteritis worldwide. Several studies have reported the predominance of G4P[6] rotavirus genotypes in Korean neonates, which is uncommon in other countries. Therefore, the purposes of this study were to determine the genotype constellations of complete genomes of G4P[6] rotavirus strains isolated from Korean neonates using next-generation sequencing, to compare these sequences with other G4P[6] strains in other countries, and to determine the reason for the predominance of G4P[6] genotypes in Korean neonates. Results Twenty rotavirus G4P[6] strains, isolated from January 2013 to January 2016, were selected for whole-genome sequencing. Eleven rotavirus genes were amplified using specific primer sets, and sequencing was carried out using an Ion S5 XL next-generation sequencing platform. Genotypes of each gene were determined, and phylogenetic analyses were performed to investigate genetic distances between genes of rotaviruses in this study and those of other rotavirus G4P[6] strains whose whole-genome sequences were previously published. All 20 rotavirus strains in this study had the same genotype: G4-P[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1, representing the Wa-like genotype constellation. BLAST searches of 20 G4P[6] rotavirus strains revealed that all G4 sequences in this study showed the highest nucleotide identity to G4 sequences of G4P[6] rotavirus strains isolated in Korea in 2008 (GenBank accession number: FJ603447). Additionally, P[6] gene sequences in this study showed the highest nucleotide identity to P[6] sequences of G4P[6] strains detected in Korea in 2002 (AY158093). Phylogenetic and nucleotide sequence analyses showed that G4P[6] strains in this study and previously reported G4P[6] strains in Korea were mostly detected in neonates and had similar G4 and P[6] sequences compared with other G4P[6] strains detected in other countries. Conclusions This study showed that the whole-genome constellation of rotavirus G4P[6] strains from Korean neonates resembled a Wa-like genotype constellation. Additionally, rotavirus genotypes detected in Korean neonates had unique P[6] sequences, which may be the cause of Korean neonatal rotavirus infection. Electronic supplementary material The online version of this article (10.1186/s13099-019-0318-5) contains supplementary material, which is available to authorized users.

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