High Prevalence of β-lactamase and Plasmid-Mediated Quinolone Resistance Genes in Extended-Spectrum Cephalosporin-Resistant Escherichia coli from Dogs in Shaanxi, China

Liu, Xiaoqiang; Liu, Haixia; Li, Yinqian; Hao, Caiju

doi:10.3389/fmicb.2016.01843

Cited by 40 publications

(31 citation statements)

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“…In E. coli belonging to several STs of companion animal origin, the gene aac(6′)Ib-cr was identified (96)(97)(98)(99). This gene was located on plasmids of the IncF family, and a bla CTX-M ESBL gene, usually bla CTX-M-15 , was often colocated (96,98).…”

Section: Resistance To (Fluoro)quinolones By Plasmid-borne Resistancementioning

confidence: 99%

“…Furthermore, aac(6′)Ib-cr was described in E. coli isolates from the feces of French cattle, where it was also colocated with bla CTX-M-15 on plasmids belonging to the IncF family (100). The gene qepA was identified in E. coli of companion animal origin belonging to different STs (97). Plasmids of the IncF family harbored qepA in E. coli from food-producing and companion animals (101).…”

Section: Resistance To (Fluoro)quinolones By Plasmid-borne Resistancementioning

confidence: 99%

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Antimicrobial Resistance inEscherichia coli

et al. 2018

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Multidrug resistance in has become a worrying issue that is increasingly observed in human but also in veterinary medicine worldwide. is intrinsically susceptible to almost all clinically relevant antimicrobial agents, but this bacterial species has a great capacity to accumulate resistance genes, mostly through horizontal gene transfer. The most problematic mechanisms in correspond to the acquisition of genes coding for extended-spectrum β-lactamases (conferring resistance to broad-spectrum cephalosporins), carbapenemases (conferring resistance to carbapenems), 16S rRNA methylases (conferring pan-resistance to aminoglycosides), plasmid-mediated quinolone resistance (PMQR) genes (conferring resistance to [fluoro]quinolones), and genes (conferring resistance to polymyxins). Although the spread of carbapenemase genes has been mainly recognized in the human sector but poorly recognized in animals, colistin resistance in seems rather to be related to the use of colistin in veterinary medicine on a global scale. For the other resistance traits, their cross-transfer between the human and animal sectors still remains controversial even though genomic investigations indicate that extended-spectrum β-lactamase producers encountered in animals are distinct from those affecting humans. In addition, of animal origin often also show resistances to other-mostly older-antimicrobial agents, including tetracyclines, phenicols, sulfonamides, trimethoprim, and fosfomycin. Plasmids, especially multiresistance plasmids, but also other mobile genetic elements, such as transposons and gene cassettes in class 1 and class 2 integrons, seem to play a major role in the dissemination of resistance genes. Of note, coselection and persistence of resistances to critically important antimicrobial agents in human medicine also occurs through the massive use of antimicrobial agents in veterinary medicine, such as tetracyclines or sulfonamides, as long as all those determinants are located on the same genetic elements.

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Section: Resistance To (Fluoro)quinolones By Plasmid-borne Resistancementioning

confidence: 99%

Section: Resistance To (Fluoro)quinolones By Plasmid-borne Resistancementioning

confidence: 99%

Antimicrobial Resistance inEscherichia coli

et al. 2018

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Section: Resistance To (Fluoro)quinolones By Chromosomal Target Sitementioning

confidence: 99%

Antimicrobial Resistance inEscherichia coli

Poirel

Madec

Lupo

et al. 2018

Antimicrobial Resistance in Bacteria From Livestock and Companion Animals

107

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Multidrug resistance in Escherichia coli has become a worrying issue that is increasingly observed in human but also in veterinary medicine worldwide. E. coli is intrinsically susceptible to almost all clinically relevant antimicrobial agents, but this bacterial species has a great capacity to accumulate resistance genes, mostly through horizontal gene transfer. The most problematic mechanisms in E. coli correspond to the acquisition of genes coding for extended-spectrum β-lactamases (conferring resistance to broad-spectrum cephalosporins), carbapenemases (conferring resistance to carbapenems), 16S rRNA methylases (conferring pan-resistance to aminoglycosides), plasmid-mediated quinolone resistance (PMQR) genes (conferring resistance to [fluoro]quinolones), and mcr genes (conferring resistance to polymyxins). Although the spread of carbapenemase genes has been mainly recognized in the human sector but poorly recognized in animals, colistin resistance in E. coli seems rather to be related to the use of colistin in veterinary medicine on a global scale. For the other resistance traits, their cross-transfer between the human and animal sectors still remains controversial even though genomic investigations indicate that extended-spectrum β-lactamase producers encountered in animals are distinct from those affecting humans. In addition, E. coli of animal origin often also show resistances to other-mostly older-antimicrobial agents, including tetracyclines, phenicols, sulfonamides, trimethoprim, and fosfomycin. Plasmids, especially multiresistance plasmids, but also other mobile genetic elements, such as transposons and gene cassettes in class 1 and class 2 integrons, seem to play a major role in the dissemination of resistance genes. Of note, coselection and persistence of resistances to critically important antimicrobial agents in human medicine also occurs through the massive use of antimicrobial agents in veterinary medicine, such as tetracyclines or sulfonamides, as long as all those determinants are located on the same genetic elements.

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“…In this study, 2686 E. coli isolates were collected from 11 water bodies between March 2015 and November 2016, with 90.9% (10/11) of sampling sites located in Guanzhong region, an economically developed and densely populated area in Shaanxi province. Generally, the prevalence rate of ESBL producers was 2.8%, which was much lower than the prevalence of ESBL producers among the E. coli isolated from dogs (24.2%), retail meat (22.3%) and pigs (9.6%, unpublished data from our group) in Shaanxi province [ 19 , 20 ]. Meanwhile, the frequency of ESBL-producing E. coli varied significantly at different sampling sites, and it was more frequently isolated in the Gaogan Canal (6.4%), Qixing River (4.3%) and Xiaowei River (4.2%) compared with Hei River (1.1%) ( P < 0.01).…”

Section: Discussionmentioning

confidence: 87%

Molecular characteristics of extended-spectrum β-lactamase-producing Escherichia coli isolated from the rivers and lakes in Northwest China

Liu

Zhou

et al. 2018

BMC Microbiol

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BackgroundExtended-spectrum β-lactamases (ESBLs)-producing Escherichia coli (E. coli) isolates in environment water become progressively a potential threat to public health, while the detailed information about the ESBL-producing E. coli isolates in the rivers and lakes in Northwest China is scarce. In the present study, it was aimed to characterize the ESBL-producing E. coli isolated from the surface waters in Northwest China.ResultsA total of 2686 E. coli isolates were obtained from eleven rivers and lakes in Northwest China to screen for ESBL producers. Seventy-six (2.8%) isolates were classified as ESBL producers, and phylogenic groups D and A accounted for 59.2% of the ESBL producers. CTX-Ms were the predominant ESBLs genotype, and they were represented by seven blaCTX-M subtypes. blaCTX-M-14 was the most prevalent specific CTX-M gene, followed by blaCTX-M-9, blaCTX-M-123, blaCTX-M-15, blaCTX-M-27, blaCTX-M-1 and blaCTX-M-65. Moreover, 54 of the 76 ESBL producers carried at least one plasmid-mediated quinolone resistance (PMQR) gene, and aac(6′)-Ib-cr was predominant. The overall occurrence of virulence factors ranged from 1.3% (eae) to 48.7% (traT). Thirty-seven sequence types (STs) were confirmed among the 76 ESBL producers, and the predominant was ST10, which was represented by 10 isolates; importantly, clone B2-ST131, associated with severe infections in humans and animals, was detected three times.ConclusionThe prevalence of ESBL-producing E. coli from the rivers and lakes in Northwest China was low (2.8%), and the extraintestinal pathogenic E. coli (ExPEC) pathotype was the most commonly detected on the basis of the virulence factor profiles. 76.3% of ESBL producers harbored more than one β-lactamase gene, and blaCTX-M-14 was the predominant genotype. Notably, one ST131 isolate from Gaogan Canal simultaneously harbored blaCTX-M-9, blaCTX-M-15, blaCTX-M-123, blaKPC-2, blaNDM-1, blaOXA-2 as well as the PMQR genes qnrA, qnrS and aac(6′)-Ib-cr.

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High Prevalence of β-lactamase and Plasmid-Mediated Quinolone Resistance Genes in Extended-Spectrum Cephalosporin-Resistant Escherichia coli from Dogs in Shaanxi, China

Cited by 40 publications

References 45 publications

Antimicrobial Resistance inEscherichia coli

Antimicrobial Resistance inEscherichia coli

Antimicrobial Resistance inEscherichia coli

Molecular characteristics of extended-spectrum β-lactamase-producing Escherichia coli isolated from the rivers and lakes in Northwest China

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