High production of butyric acid by Clostridium tyrobutyricum mutant

Ou, Jianfa; Miller, Matthew; McFann, Sarah; Liu, Margaret

doi:10.1007/s11705-015-1525-3

Cited by 11 publications

(4 citation statements)

References 27 publications

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“…Nevertheless, the final product purity (butyric acid/total acids) obtained from these fermentations was ∼0.99, which was a dramatic improvement over the control (0.79 for BV 0 at pH 6.0). Overall, the best butyrate production, including higher final butyrate concentration, yield and productivity, was obtained at pH 6.5, which could be attributed to the alleviated product inhibition caused by butyric acid at the higher pH value (Ma et al, 2015; Michel‐Savin et al, 1990; Zhu & Yang, 2003).…”

Section: Resultsmentioning

confidence: 99%

Effects of benzyl viologen on increasing NADH availability, acetate assimilation, and butyric acid production by Clostridium tyrobutyricum

Meng

Tang³

et al. 2020

Biotech & Bioengineering

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Clostridium tyrobutyricum produces butyric and acetic acids from glucose. The butyric acid yield and selectivity in the fermentation depend on NADH available for acetate reassimilation to butyric acid. In this study, benzyl viologen (BV), an artificial electron carrier that inhibits hydrogen production, was used to increase NADH availability and butyric acid production while eliminating acetic acid accumulation by facilitating its reassimilation. To better understand the mechanism of and find the optimum condition for BV effect on enhancing acetate assimilation and butyric acid production, BV at various concentrations and addition times during the fermentation were studied. Compared with the control without BV, the addition of 1 μM BV increased butyric acid production from glucose by ∼50% in yield and ∼29% in productivity while acetate production was completely inhibited. Furthermore, BV also increased the coutilization of glucose and exogenous acetate for butyric acid production. At a concentration ratio of acetate (g/L) to BV (mM) of 4, both acetate assimilation and butyrate biosynthesis increased with increasing the concentrations of BV (0–6.25 μM) and exogenous acetate (0–25 g/L). In a fed‐batch fermentation with glucose and ∼15 g/L acetate and 3.75 μM BV, butyrate production reached 55.9 g/L with productivity 0.93 g/L/h, yield 0.48 g/g, and 97.4% purity, which would facilitate product purification and reduce production cost. Manipulating metabolic flux and redox balance via BV and acetate addition provided a simple to implement metabolic process engineering approach for butyric acid production from sugars and biomass hydrolysates.

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Section: Resultsmentioning

confidence: 99%

Effects of benzyl viologen on increasing NADH availability, acetate assimilation, and butyric acid production by Clostridium tyrobutyricum

Meng

Tang³

et al. 2020

Biotech & Bioengineering

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“…The genus Clostridium consists of approximately 180 species, a few of which are pathogenic, producing very harmful toxins. However, Clostridium species are also used in biotechnological and therapeutic applications [ 73 , 74 , 75 , 76 , 77 ]. Many clostridia have been reported to produce butyric acid—a short-chain fatty acid (SCFA)—as a major fermentation product.…”

Section: Discussionmentioning

confidence: 99%

Silk-derived sericin/fibroin mixture drink fermented with plant-derived <i>Lactococcus lactis</i> BM32-1 improves constipation and related microbiota: a randomized, double-blind, and placebo-controlled clinical trial

NODA,

DANSHIITSOODOL,

KANNO

et al. 2024

Bioscience of Microbiota, Food and Health

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We previously showed through clinical trials that one plant-derived lactic acid bacteria (LAB) can improve constipation. We preliminarily found that the plant-derived LAB Lactococcus lactis BM32-1 can grow in a mixture of sericin and fibroin, which are extracted from silk and have been reported to help promote health. Thus, in the present study, we evaluated the favorable effect of a sericin/fibroin mixture (S/F-M), which was extracted from silk prepared from cocoons reared in an aseptic rearing system using an artificial diet, fermented with the BM32-1 strain through a clinical trial. The trial was conducted at Hiroshima University from June to October 2022 as a double-blind, placebo-controlled, randomized parallel-group comparative study with 50 eligible subjects (aged 23-71) who had an average defecation frequency of less than 5 times per week. The subjects were instructed to drink 100 mL of fermented S/F-M or placebo every day. After the 12 weeks of the clinical trial period, the average defecation frequency increased significantly-1.4 times higher than that at baseline in the test group-as compared with the placebo group. Furthermore, the fecal microbiota was also compared before and after treatment, revealing that intake of the fermented S/F-M significantly multiplied the relative abundance of the genera Enterococcus and Clostridium, which have been reported to contribute to the amelioration of constipation by improving the gut microbiota and producing butyric acid, respectively. In conclusion, the S/F-M fermented using the BM32-1 strain improves defecation frequency through alteration of the gut microbiota.

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“…Our recent butyrate fermentation using C. tyrobutyricum showed that the fermentation pH significantly affected the production of butyrate and cell growth [ 33 ]. Specifically, the immobilized-cell fermentations at pH 6.0, 6.5 and 7.0 produced butyrate with concentrations of 50.11 g/L, 63.02 g/L and 61.01 g/L.…”

Section: Discussionmentioning

confidence: 99%

“…To effectively integrate cell engineering and process engineering, metabolomics should be developed and applied to C. tyrobutyricum . Metabolic flux analysis modeling is also an effective tool to guide the design of metabolic engineering [ 33 , 34 ]. In addition, the medium components can be rationally designed to assist in butanol production post cell engineering.…”

Section: Discussionmentioning

confidence: 99%

Rebalancing Redox to Improve Biobutanol Production by Clostridium tyrobutyricum

Fierst

et al. 2015

Bioengineering

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Biobutanol is a sustainable green biofuel that can substitute for gasoline. Carbon flux has been redistributed in Clostridium tyrobutyricum via metabolic cell engineering to produce biobutanol. However, the lack of reducing power hampered the further improvement of butanol production. The objective of this study was to improve butanol production by rebalancing redox. Firstly, a metabolically-engineered mutant CTC-fdh-adhE2 was constructed by introducing heterologous formate dehydrogenase (fdh) and bifunctional aldehyde/alcohol dehydrogenase (adhE2) simultaneously into wild-type C. tyrobutyricum. The mutant evaluation indicated that the fdh-catalyzed NADH-producing pathway improved butanol titer by 2.15-fold in the serum bottle and 2.72-fold in the bioreactor. Secondly, the medium supplements that could shift metabolic flux to improve the production of butyrate or butanol were identified, including vanadate, acetamide, sodium formate, vitamin B12 and methyl viologen hydrate. Finally, the free-cell fermentation produced 12.34 g/L of butanol from glucose using the mutant CTC-fdh-adhE2, which was 3.88-fold higher than that produced by the control mutant CTC-adhE2. This study demonstrated that the redox engineering in C. tyrobutyricum could greatly increase butanol production.

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High production of butyric acid by Clostridium tyrobutyricum mutant

Cited by 11 publications

References 27 publications

Effects of benzyl viologen on increasing NADH availability, acetate assimilation, and butyric acid production by Clostridium tyrobutyricum

Effects of benzyl viologen on increasing NADH availability, acetate assimilation, and butyric acid production by Clostridium tyrobutyricum

Silk-derived sericin/fibroin mixture drink fermented with plant-derived <i>Lactococcus lactis</i> BM32-1 improves constipation and related microbiota: a randomized, double-blind, and placebo-controlled clinical trial

Rebalancing Redox to Improve Biobutanol Production by Clostridium tyrobutyricum

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