High Production of Cellulase and Xylanase in Solid-State Fermentation by Trichoderma reesei Using Spent Copra and Wheat Bran in Rotary Bioreactor

Abstract

The enzyme production for lignocellulose saccharification by solid-state fermentation (SSF) of a food manufacturing byproduct was successfully carried out in a 30 L rotary bioreactor. Defatted spent copra (SC) supplemented with wheat bran (WB) was used as a substrate for the SSF of Trichoderma reesei and aerated at various rates. Regression analysis showed that the carbohydrate/protein (C/P) ratio of the substrate and the supplied aeration rate were the important factors for producing the enzyme cocktail, including cellulases (FPase, CMCase, and cellobiase) and xylanase. The substrate containing SC:WB of 3:2 (or the C/P ratio of 5.4) and the aeration of 1.0 L kg −1 substrate min −1 were found to enhance the production of the enzymes up to 5.68, 8.66, 29.2, and 34.44 U g −1 of dry substrate for FPase, CMCase, cellobiase, and xylanase activities, respectively. This discovery provided a promising environment for other substrates to produce multi-enzymes for lignocellulosic saccharification. Additionally, mathematical models were generated to predict the saccharifying degree of the produced enzyme for lignocellulose saccharification.

Discovery of a Novel β-xylosidase with Xylanase Activity and Its Application in the Production of Xylitol from Corncob Xylan

“…In contrast, XYL4 was not only able to hydrolyze a variety of xylans, it also had the highest hydrolyzing activity for corncob xylan. Although xylanases with high activity have been identified [28], the hydrolysis of xylan is still inseparable from the synergy of β-xylosidase [8]. Therefore, β-xylosidase XYL4 with xylanase activity shows a broad potential application in the saccharification of hemicellulose xylan.…”

Assessment of the Techno-Economic Feasibility of Semicontinuous Production of Metarhizium anisopliae Spores

“…However, despite the removal of metabolic heat not having impaired production, this is the major challenge for scaling up these bioreactors, along with production uniformity along the bed. 20 Thus, assuming that the technical drawbacks concerning the cultivation of M. anisopliae in packed-bed bioreactors could be overcome, a process more robust for the production of fungal spores can be proposed. In this context, this study aims to propose the development of an optimized industrial process to produce spores of M. anisopliae and evaluate its technical and economic feasibility based on cultivation in packed-bed bioreactors.…”

“…However, it is mandatory to take into account the reported scaling-up challenges related to the compaction of the culture medium, temperature increase, and subsequent drying of the cultivation medium. , Da Cunha et al scaled-up packed-bed bioreactors by increasing the bioreactor diameter 3-fold, observing a temperature increase of 5 °C above the optimal temperature without impairing fungal growth. However, despite the removal of metabolic heat not having impaired production, this is the major challenge for scaling up these bioreactors, along with production uniformity along the bed …”