High production yield and specific productivity of succinate from cassava starch by metabolically-engineered<i>Escherichia coli</i>KJ122

Khor, Kirin; Sawisit, Apichai; Chan, Sitha; Kanchanatawee, Sunthorn; Jantama, Sirima Suvarnakuta; Jantama, Kaemwich

doi:10.1002/jctb.4893

Cited by 10 publications

(4 citation statements)

References 30 publications

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“…For example, succinic acid and 1,4‐BDO, both of which are used for the synthesis of PBS as monomers, have successfully been produced by employing metabolically engineered microorganisms in significant yields suitable for commercial production (Table 1 and Figure 2). [ 57–74 ] In particular, the production of succinic acid is currently being replaced by biological production and several companies already produce bio‐succinic acid in an industrial scale. [ 94,100 ] Similar to PBS, all monomers of PBAT, such as 1,4‐BDO, adipic acid, and even TPA, can also be produced by engineered microbial strains (Table 1 and Figure 2).…”

Section: Biodegradable Biopolymersmentioning

confidence: 99%

Recent Advances in Sustainable Plastic Upcycling and Biopolymers

Sohn

Kim

Baritugo

et al. 2020

Biotechnology Journal

116

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Advances in scientific technology in the early twentieth century have facilitated the development of synthetic plastics that are lightweight, rigid, and can be easily molded into a desirable shape without changing their material properties. Thus, plastics become ubiquitous and indispensable materials that are used in various manufacturing sectors, including clothing, automotive, medical, and electronic industries. However, strong physical durability and chemical stability of synthetic plastics, most of which are produced from fossil fuels, hinder their complete degradation when they are improperly discarded after use. In addition, accumulated plastic wastes without degradation have caused severe environmental problems, such as microplastics pollution and plastic islands. Thus, the usage and production of plastics is not free from environmental pollution or resource depletion. In order to lessen the impact of climate change and reduce plastic pollution, it is necessary to understand and address the current plastic life cycles. In this review, "sustainable biopolymers" are suggested as a promising solution to the current plastic crisis. The desired properties of sustainable biopolymers and bio-based and bio/chemical hybrid technologies for the development of sustainable biopolymers are mainly discussed.

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Section: Biodegradable Biopolymersmentioning

confidence: 99%

Recent Advances in Sustainable Plastic Upcycling and Biopolymers

Sohn

Kim

Baritugo

et al. 2020

Biotechnology Journal

116

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“…The enzymatic hydrolysis condition was investigated to ensure complete hydrolysis of the cassava starch to fermentable sugars. From previous works, the steps of gelatinization and hydrolysis using α‐amylase were performed at the same time 29,30 . Therefore, the experiment was first conducted by the addition of α‐amylase enzyme before gelatinization.…”

Section: Resultsmentioning

confidence: 99%

“…In general, a conventional biochemical production process from starch requires gelatinization and liquefaction followed by the saccharification step with high amounts of α‐amylase and glucoamylase to completely hydrolyze starch to glucose prior to fermentation. In the conventional process, Khor et al 30 . revealed that the liquefaction step was usually initiated by adding an α‐amylase enzyme to the cassava starch slurry before autoclaving.…”

Section: Discussionmentioning

confidence: 99%

Sequential coupling of enzymatic hydrolysis and fermentation platform for high yield and economical production of 2,3‐butanediol from cassava by metabolically engineered Klebsiella oxytoca

Khunnonkwao

Jantama

et al. 2020

J of Chemical Tech & Biotech

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BACKGROUND 2,3‐butanediol (2,3‐BD) is one of the important platform chemicals used for biofuels and biochemicals. To make an economically feasible production of 2,3‐BD, an optimization process with a cheap substrate is required. The best conditions for enzymatic hydrolysis and fermentation were investigated to achieve high concentration and yield of 2,3‐BD from cassava starch while reducing the production cost regarding enzyme usage, energy input and medium preparation. RESULTS A 2.5 L bioreactor containing 1 L working volume of sterilized and gelatinized starch (130 g L−1) was placed at room temperature (25 °C). When the temperature of the starch solution reached 85 °C, α‐amylase (200 U g−1 starch) was added to the solution. The enzymatic reaction was allowed to proceed while the temperature of the solution was simultaneously dampened to 45 °C within 1 h. The reaction was further maintained at 45 °C for another 2 h. After hydrolysis, 64.8 g L−1 2,3‐BD was produced with a yield of 0.46 g g−1, productivity of 1.25 g L−1 h−1 and few by‐products by Klebsiella oxytoca KMS006. Further improvements of 2,3‐BD (92.5 g L−1) and yield (0.49 g g−1) were observed in fed‐batch mode under a constant feeding rate of 3.5 g starch h−1. CONCLUSION With our developed strategy, costs related to energy consumption and medium preparation were reduced by using heat generated by autoclave as well as by neglecting the use of glucoamylase for hydrolysis of cassava starch. The highest yield of 2,3‐BD (0.49 g g−1) was achieved, which is 98% of theoretical maximum. The process may be applied to the economical production of 2,3‐BD. © 2020 Society of Chemical Industry

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“…Bio-based succinate is mostly produced in a monosaccharide-based biorefinery platform, using microorganisms such as Actinobacillus succinogenes, Anaerobiospirillum succiniciproducens, Mannheimia succiniciproducens, and a recombinant Escherichia coli strain (Bozell and Petersen, 2010;Rakshit, 2004). Succinate production from cassava starch has been tested using metabolically-engineered Escherichia coli KJ122, but a biological pretreatment by adding hydrolytic enzyme was required (Khor et al, 2016). So far, succinate production from biomass-derived hydrolysates has not been established (Salvachúa et al, 2016).…”

Section: Characteristic / Strainmentioning

confidence: 99%

Organic acid production from starchy waste by gut derived microorganisms

Ayudthaya¹

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Parameter Chemical routes Non-renewable feedstockspetrochemicals Fermentative routes Biobased feedstockcarbohydrates Price considerations Still cheaper than the renewable sources. Feedstock themselves do not contribute to the price as much as downstream processing Availability Availability expected to decrease in time Abundant and renewable Routes Developed routes, established technologies Routes under constant improvement, young technologies Yields and productivities Generally high Sometimes a large number of side products, diluted media, long reaction times Major disadvantages High energy demands (pressure and temperature). Catalysts disposal issues Sensitivity of microorganisms, nutrient requirements, complicated product recovery, large amounts of waste Environment effect Release CO2 that contributes to global warming Consume CO2 via TCA cycle Public awareness Decreasing popularity Increased interest in improving currently applied routes and innovations Current high costs of production prevent bio-based chemicals to be widely used. Thus, production processes from bio-based resources need to be developed and optimized. It is estimated that in 2050 approximately 30% (by weight) of chemicals will be obtained from renewable biomass (http://www.suschem.org) (Fiorentino and Ripa, 2017). Biomass is an abundant carbon-neutral renewable resource that can be used as a carbon source instead of fossil feedstocks (Fiorentino and Ripa, 2017). Production chains resulting from biomass are considered as "short-cycle carbon systems", which are more sustainable and preferable than those resulting from fossil resources, which are considered as "long-cycle carbon systems" (Kajaste et al., 2014; Fiorentino and Ripa, 2017). The transition from fossil based to bio-based economy needs the development of innovative processes to exploit the potential of biomass (Pleissner et al., 2017). Thus, utilization of food wastes or biomass and their conversion into valuable products require more attention as the implementation of such system is not yet successfully guaranteed. However, many criteria need to be considered for * Guyot et al. (2000) * Narita et al. 2004 Porphyromonadaceae. In the reactor with the Thai cow inoculum, Streptococcus spp. also increased from the start. When lactate was consumed, acetate, propionate and butyrate were produced (day 3-4). After day 3, bacteria belonging to five dominant groups, Bacteroides, Pseudoramibacter_Eubacterium, Dysgonomonas, Enterobacteriaceae and Porphyromonadaceae were detected and these showed significant positive correlations with acetate, propionate and butyrate levels. The complexity of rumen microorganisms with high adaptation capacity makes rumen fluid a suitable source to convert organic waste into valuable products without the addition of hydrolytic enzymes. Starch waste is a source for organic acid production, especially lactate.

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High production yield and specific productivity of succinate from cassava starch by metabolically-engineeredEscherichia coliKJ122

Cited by 10 publications

References 30 publications

Recent Advances in Sustainable Plastic Upcycling and Biopolymers

Recent Advances in Sustainable Plastic Upcycling and Biopolymers

Sequential coupling of enzymatic hydrolysis and fermentation platform for high yield and economical production of 2,3‐butanediol from cassava by metabolically engineered Klebsiella oxytoca

Organic acid production from starchy waste by gut derived microorganisms

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