High-quality draft genome sequence of Sedimenticola selenatireducens strain AK4OH1T, a gammaproteobacterium isolated from estuarine sediment

Abstract

Sedimenticola selenatireducens strain AK4OH1T (= DSM 17993T = ATCC BAA-1233T) is a microaerophilic bacterium isolated from sediment from the Arthur Kill intertidal strait between New Jersey and Staten Island, NY. S. selenatireducens is Gram-negative and belongs to the Gammaproteobacteria. Strain AK4OH1T was the first representative of its genus to be isolated for its unique coupling of the oxidation of aromatic acids to the respiration of selenate. It is a versatile heterotroph and can use a variety of carbon compounds, but can also grow lithoautotrophically under hypoxic and anaerobic conditions. The draft genome comprises 4,588,530 bp and 4276 predicted protein-coding genes including genes for the anaerobic degradation of 4-hydroxybenzoate and benzoate. Here we report the main features of the genome of S. selenatireducens strain AK4OH1T.

Taxonomic and functional heterogeneity of the gill microbiome in a symbiotic coastal mangrove lucinid species

“…Thiodiazotropha endolucinida [13] and Sedimenticola spp. [48, 49, 118] (Table 1). FISH using a newly designed SED642 probe targeting the 16S rRNA gene of this Sedimenticola -like species confirmed that the P. pectinatus gill bacteriocytes contained cells that matched the gammaproteobacterial MAGs (Figure S3).…”

“…The Sedimenticola -like MAGs shared 71 ± 4% ANI and 64 ± 1% AAI with sequenced clade A lucinid symbiont species, 76 ± 7% ANI and 59 ± 5% AAI with other marine thioautotrophic symbionts, and 76 ± 2% ANI and 69 ± 1% AAI to free-living Sedimenticola spp. [48, 49, 118] (Figure S2a). Based on the 93–95% ANI and 85–90% AAI boundaries proposed in Rodriguez-R and Konstantinidis [50], the Sedimenticola -like MAGs were likely a species separate from sequenced clade A lucinid symbionts, marine thioautotrophic symbionts, and Sedimenticola spp.…”

“…Sedimenticola endophacoides, free-living Sedimenticola spp. [48, 118], and bacterial symbionts, including clade A thioautotrophic lucinid symbionts the lucinid thioautotrophic symbiont clade A [13, 14], a vesicomyid clam gill symbiont ( Ca . Ruthia magnifica) [119], a solemyid clam gill symbiont [97], bathymodiolin mussel gill symbionts ( Bathymodiolus septemdierum and Bathymodiolus thermophilus ) [75, 120], deep-sea tubeworm trophosomal symbionts ( Ridgeia piscesae , Riftia pachyptila, and Tevnia jerichonana ) [93, 121], a marine nematode ectosymbiont ( Ca.…”

Occurrence and diversity of the oxidative hydroxyhydroquinone pathway for the anaerobic degradation of aromatic compounds in nitrate‐reducing bacteria

“…S. selenatireducens is an anaerobic selenate respiring bacterium that can also use nitrate as terminal electron acceptor. The strain AKOH1, isolated from estuarine sediments, bears the genetic complement to grow with benzoate and hydroxybenzoates as substrates using the benzoyl‐CoA central pathway, as does the CUZ strain (Narasingarao and Häggblom, ; Louie et al, ). The presence in the genome of this strain of the hydroxyhydroquinone pathway detected in this analysis suggests that it may also use dihydroxyaromatics through hydroxyhydroquinone as the central intermediate.…”

Integrated multi-omics investigations reveal the key role of synergistic microbial networks in removing plasticizer di-(2-ethylhexyl) phthalate from estuarine sediments

“…Putative DEHP-degrading hydrolase (k141_138169_2) and β-oxidation genes for anaerobic 2-ethylhexanol degradation were identified in Bin14, and its closest taxonomy affiliation was Sedimencola selenatireducens . The degradation of DEHP and other PAEs has not been previously reported for the genus Sedimenticola [30–33]. Their apparent enrichment in DEHP-treated sediments suggests that Sedimenticola plays a role in DEHP degradation.…”