High-Quality Lipoaspirate Following 1470-nm Radial Emitting Laser-Assisted Liposuction

Abstract

Background: Laser-assisted liposuction (LAL) has been used to maximize viable adipocyte yields in lipoaspirates, although optimizing tissue processing methods is still a challenge. A high-quality lipoaspirate has been a key factor for extended graft longevity. Objective: To assess the viability and potency of stromal vascular fraction (SVF) cells and adipose-derived stem cells (ASCs) in fat samples from lipoaspirates harvested with a novel 1470-nm diode, radial emitting LAL platform. Two processing methods, enzymatic and nonenzymatic, were compared. Methods: Laser-assisted liposuction lipoaspirates harvested from 10 subjects were examined for cell viability after processing by enzymatic or nonenzymatic methods. Isolated SVF cells were cultured with an ASC-permissive medium to assess their viability and proliferation capacity by cell proliferation assay. Flow cytometric analysis with ASC-specific markers, gene expression levels, and immunofluorescence for ASC transcription factors were also conducted. Results: Lipoaspirates showed high SVF cell viability of 97% ± 0.02% and 98% ± 0.01%, averaged SVF cell count of 8.7 Â 10 6 ± 3.9 Â 10 6 and 9.4 Â 10 6 ± 4.2 Â 10 6 cells per mL, and averaged ASC count of 1 Â 10 6 ± 2.2 Â 10 5 and 1.2 Â 10 6 ± 5 Â 10 5 cells per mL in nonenzymatic and enzymatic methods, respectively. The ASC-specific markers, gene expression levels, and immunofluorescence for ASC transcription factors confirmed the adipose origin of the cells. Conclusions: The laser lipoaspirates provide a high yield of viable and potent SVF cells and ASCs through both nonenzymatic and enzymatic processes. Improved purity of the harvested lipoaspirate and high ASC content are expected to result in extended graft longevity. Furthermore, eliminating enzymatic digestion may provide advantages, such as reducing process time, cost, and regulatory constraints.

Multifrequency Laser-Assisted Lipolysis in a Patient with Excessive Redundant Skin Post Roux-en-Y Gastric Bypass: A Clinical Case and Literature Review

“…Since its Food and Drug Administration (FDA) approval in 2006, there has been an explosive development of medical lasers with different wavelengths (924, 968, 980, 1 064, 1 319, 1 320, 1 344, and 1 440 nm) that have eventually been employed for lipolysis and skin tightening (35)(36)(37)(38)(39) and mostly studies have supported the initial clinical observations in adiposity reduction, shorter recovery times, and improved skin retraction (40)(41)(42)(43). In this regard, the Lipolaser LPL9002™ device employed in this clinical case has the advantage of three lowpower cold lasers at wavelengths of 532, 650, and 980 nanometers, with no need for external cooling during the surgery, thus reducing the burn Panel 2.…”

What We Know About Facial Volume Restoration with Autogenous Fat

“…There are some techniques that are based on the use of liposuction machines, some based on hand syringe techniques, while the Alma BeautiFill (Alma Lasers-Bloomedical, Breda, Nederland) device depends on a 1,470-nm laser-assisted cannula. 23 All of these harvesting methods have strong advocates, each claim-ing sufficient viability of the fat cells for transfer. A key principle is that the fat cells should be handled as gently as possible.…”

Applicability of the 1470 Nm Diode Laser in Facial Aesthetics: An Evidence-Based Review

“…This interaction facilitates the "evaporation of tissue water," a phenomenon contingent on the fluence applied per cubic centimetre of tissue. A thermal effect ensues upon absorption of tissue water, inducing the retraction of collagen fibres, lipolysis, neocollagenesis, and neovascularisation [14][15][16][17][18].…”

“…For a long period, the predominant procedures performed with the 1470 nm diode laser were exclusively intended for medical treatment, such as laser-assisted liposuction for gynecomastia [34], laser-assisted liposuction for body contouring and facial fat grafting [14,15], ablation of large saphenous veins [16], pilonidal sinus disease [17], vascular lesions [18], and other medical conditions. Nonetheless, this technology has opened a new paradigm of applicability in the field of facial aesthetics, as this wavelength emits light energy preferentially absorbed by water, producing a rapid contouring effect in addition to reducing adipose tissue, providing skin firmness and aesthetic improvement [14].…”