2022
DOI: 10.3389/fgene.2022.995072
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High-quality reference transcriptome construction improves RNA-seq quantification in Oryza sativa indica

Abstract: The Reference Transcriptomic Dataset (RTD) is an accurate and comprehensive collection of transcripts originating from a given organism. It holds the key to precise transcript quantification and downstream analysis of differential expressions and regulations. Currently, transcriptome annotations for most crop plants are far from complete. For example, Oryza sativa indica (O. sativa indica) is reported to have 40,759 transcripts in the Ensembl database without alternative transcript isoforms and alternative spl… Show more

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