High-Quality Tissue Imaging Using a Catheter-Based Swept-Source Optical Coherence Tomography Systems With an Integrated Semiconductor Optical Amplifier

Mao, Youxin; Flueraru, Costel; Chang, Shoude; Popescu, Dan P.; Sowa, Michael G.

doi:10.1109/tim.2011.2126950

Cited by 23 publications

(4 citation statements)

References 23 publications

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“…35 Although single dose of 40 Gy is not clinically used (cumulative dose ranges between 50 and 70 Gy, fractionated over 5 to 7 weeks), it was considered appropriate to simulate severe radiation injury and achieve RIF with telangiectasia in mice within first 6 months after treatment, based on previous preclinical experience. 36,37 A swept-source OCT system based on a quadrature interferometer to suppress the complex conjugate artifact 38,39 was used in this study [ 110 nm and an average output power of 10 mW. The axial and lateral resolutions in air were 8 and 15 μm, respectively.…”

Section: Methodsmentioning

confidence: 99%

Preclinical quantitative in-vivo assessment of skin tissue vascularity in radiation-induced fibrosis with optical coherence tomography

et al. 2018

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Radiation therapy (RT) is widely and effectively used for cancer treatment but can also cause deleterious side effects, such as a late-toxicity complication called radiation-induced fibrosis (RIF). Accurate diagnosis of RIF requires analysis of histological sections to assess extracellular matrix infiltration. This is invasive, prone to sampling limitations, and thus rarely used; instead, current practice relies on subjective clinical surrogates, including visual observation, palpation, and patient symptomatology questionnaires. This preclinical study demonstrates that functional optical coherence tomography (OCT) is a useful tool for objective noninvasive in-vivo assessment and quantification of fibrosis-associated microvascular changes in tissue. Data were collected from murine hind limbs 6 months after 40-Gy single-dose irradiation and compared with nonirradiated contralateral tissues of the same animals. OCT-derived vascular density and average vessel diameter metrics were compared to quantitative vascular analysis of stained histological slides. Results indicate that RIF manifests significant microvascular changes at this time point posttreatment. Abnormal microvascular changes visualized by OCT in this preclinical setting suggest the potential of this label-free high-resolution noninvasive functional imaging methodology for RIF diagnosis and assessment in the context of clinical RT.

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Section: Methodsmentioning

confidence: 99%

Preclinical quantitative in-vivo assessment of skin tissue vascularity in radiation-induced fibrosis with optical coherence tomography

et al. 2018

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“…The OCT system was based on a quadrature Mach-Zehnder interferometer with optical amplification [34], incorporating a 1320 nm light source (HS2000-HL, Santec, Komaki, Japan) and 20 kHz polygon-based tunable filter, with a sweeping range of 110 nm and average output power of 10 mW.…”

Section: Pdt Vascular Effectsmentioning

confidence: 99%

Dual-Agent Photodynamic Therapy with Optical Clearing Eradicates Pigmented Melanoma in Preclinical Tumor Models

Pires

Demidov

Wilson

et al. 2020

Cancers

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Treatment using light-activated photosensitizers (photodynamic therapy, PDT) has shown limited efficacy in pigmented melanoma, mainly due to the poor penetration of light in this tissue. Here, an optical clearing agent (OCA) was applied topically to a cutaneous melanoma model in mice shortly before PDT to increase the effective treatment depth by reducing the light scattering. This was used together with cellular and vascular-PDT, or a combination of both. The effect on tumor growth was measured by longitudinal ultrasound/photoacoustic imaging in vivo and by immunohistology after sacrifice. In a separate dorsal window chamber tumor model, angiographic optical coherence tomography (OCT) generated 3D tissue microvascular images, enabling direct in vivo assessment of treatment response. The optical clearing had minimal therapeutic effect on the in control, non-pigmented cutaneous melanomas but a statistically significant effect (p < 0.05) in pigmented lesions for both single- and dual-photosensitizer treatment regimes. The latter enabled full-depth eradication of tumor tissue, demonstrated by the absence of S100 and Ki67 immunostaining. These studies are the first to demonstrate complete melanoma response to PDT in an immunocompromised model in vivo, with quantitative assessment of tumor volume and thickness, confirmed by (immuno) histological analyses, and with non-pigmented melanomas used as controls to clarify the critical role of melanin in the PDT response. The results indicate the potential of OCA-enhanced PDT for the treatment of pigmented lesions, including melanoma.

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“…A swept-source OCT system based on a quadrature interferometer to suppress the complex conjugate artifact [37,38] was used in this study (Fig. 1).…”

Section: Optical Coherence Tomography Systemmentioning

confidence: 99%

Analysis of low-scattering regions in optical coherence tomography: applications to neurography and lymphangiography

Demidov

Matveev

Demidova

et al. 2019

Biomed. Opt. Express

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Analysis of semi-transparent low scattering biological structures in optical coherence tomography (OCT) has been actively pursued in the context of lymphatic imaging, with most approaches relying on the relative absence of signal as a means of detection. Here we present an alternate methodology based on spatial speckle statistics, utilizing the similarity of a distribution of given voxel intensities to the power distribution function of pure noise, to visualize the low-scattering biological structures of interest. In a human tumor xenograft murine model, we show that these correspond to lymphatic vessels and nerves; extensive histopathologic validation studies are reported to unequivocally establish this correspondence. The emerging possibility of OCT lymphangiography and neurography is novel and potentially impactful (especially the latter), although further methodology refinement is needed to distinguish between the visualized lymphatics and nerves.

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High-Quality Tissue Imaging Using a Catheter-Based Swept-Source Optical Coherence Tomography Systems With an Integrated Semiconductor Optical Amplifier

Cited by 23 publications

References 23 publications

Preclinical quantitative in-vivo assessment of skin tissue vascularity in radiation-induced fibrosis with optical coherence tomography

Preclinical quantitative in-vivo assessment of skin tissue vascularity in radiation-induced fibrosis with optical coherence tomography

Dual-Agent Photodynamic Therapy with Optical Clearing Eradicates Pigmented Melanoma in Preclinical Tumor Models

Analysis of low-scattering regions in optical coherence tomography: applications to neurography and lymphangiography

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