High rate of detection of G8P[6] rotavirus in children with acute gastroenteritis in São Tomé and Príncipe

Istrate, Claudia; Sharma, Sumit; Nordgren, Johan; Castro, Sandra Videira e; Lopes, Ângela Celis de Almeida; Piedade, João; Zaky, Ahmed; Lima, António; Neves, Edgar; Veiga, José Carlos Esteves; Esteves, Aida

doi:10.1007/s00705-014-2244-7

Cited by 12 publications

(14 citation statements)

References 25 publications

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“…This study has demonstrated a high detection rate of RVA and NoV in children hospitalized for diarrhea during 1 year in Burkina Faso, before the introduction of RVA vaccination. RVA was found in a total of (44%) the cases, confirming its role as the main etiological agent in severe pediatric diarrhea in Burkina Faso before the introduction of RVA vaccination, with detection rates similar to other studies in West Africa pre–RVA vaccination . NoV was found in 23% of the hospitalized cases, demonstrating a major impact of NoV in severe pediatric diarrhea, likely to become even larger, when the RVA vaccination programs were implemented, as has been reported from other countries .…”

Section: Discussionsupporting

confidence: 82%

Rotavirus and norovirus in children with severe diarrhea in Burkina Faso before rotavirus vaccine introduction

Bonkoungou

Ouédraogo

Tamini

et al. 2018

Journal of Medical Virology

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Burkina Faso introduced rotavirus vaccine (RotaTeq) to the national immunization program in November 2013. This study describes the detection rates, clinical profiles, and molecular epidemiology of rotavirus and norovirus (NoV) infections among children <5 years hospitalized (n = 154) because of acute diarrhea in Ouagadougou, Burkina Faso, from December 2012 to November 2013, just before the start of vaccination. Overall, 44% and 23% of fecal samples were positive for rotavirus and NoV, respectively, most of them detected during the cold dry season (December-March). The predominant G/P combinations were G12P[8] (47%) and G6P[6] (30%). G2P[4] (n = 3), G12P[6] (n = 3), and G6P[8] (n = 1) were also detected. Nearly all (94%) successfully genotyped NoV strains belonged to genotype GII.4. The predominance of rotavirus and NoV was noteworthy in the age group ≤6 months, with 67% rotavirus and 22% NoV, respectively. Vomiting was significantly more common among rotavirus-infected children. To conclude, this study shows high detection rates of both rotavirus and NoV in children with severe diarrhea in Burkina Faso just before the introduction of rotavirus group A vaccination. The results can be used for estimating the impact of rotavirus group A vaccination, which started in the end of 2013. Furthermore, this study shows that the G6P[6] rotavirus strains emerging in Burkina Faso in 2010 is now established as a regionally important genotype.

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Section: Discussionsupporting

confidence: 82%

Rotavirus and norovirus in children with severe diarrhea in Burkina Faso before rotavirus vaccine introduction

Bonkoungou

Ouédraogo

Tamini

et al. 2018

Journal of Medical Virology

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“…Although P[6] was initially considered of zoonotic origin [18] and assigned to SGI [18, 57], the combination G1P[6] was shown to belong to SGII, as also shown before in the previous Angolan study [22], and in the same subgroup as the well-studied G1P[8]. …”

Section: Discussionmentioning

confidence: 75%

“…Several studies conducted in Africa identified G1P[8] as the most frequent RVA strain, while the most common strains for high- and middle-income countries in the pre vaccination era, G2P[4], G3P[8], G4P[8], and G9P[8] [15], have also been identified, but to a much lesser extent [16, 17]. Noteworthy, is the emergence of uncommon strains, such as G1P[6], G8P[6], G6P[6], G8P[8], G12P[6], and mixed G and P-strains, in sub-Saharan Africa [18–21]. This picture is still far from completed for the majority of African countries, e.g.…”

Section: Introductionmentioning

confidence: 99%

Characterization of rotavirus infection in children with acute gastroenteritis in Bengo province, Northwestern Angola, prior to vaccine introduction

Gasparinho¹,

Piedade

Mirante³

et al. 2017

PLoS ONE

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BackgroundRotavirus group A (RVA) is considered the leading cause of pediatric diarrhea, responsible for the high burden of diarrheal diseases in sub-Saharan Africa. Despite recent studies, the existent data are scarce for some African countries like Angola, a country with one of the highest RVA-related death estimates. The aim of this study was to determine the RVA detection rate and circulating genotypes in children less than five years of age with acute gastroenteritis attended at the Bengo General Hospital in Caxito, Bengo province, Angola, before vaccine introduction.MethodsBetween September 2012 and December 2013, 342 fecal specimens were collected from children enrolled. Positive samples for RVA by immunochromatographic rapid test were G and P-typed by hemi-nested type-specific multiplex PCR, and subgrouped for the VP6 gene. VP4 and VP7 genes from a subset of samples were sequenced for phylogenetic analysis.ResultsDuring the study period, a high RVA detection rate was registered (25.1%, 86/342).The age group most affected by RVA infection includes children under 6 months of age (p<0.01). Vomiting was highly associated with RVA infection (72.1%; p<0.001).From the 86 RVA-positive samples, 72 (83.7%) were genotyped. The most prevalent genotype was G1P[8] (34/72; 47.2%), followed by the uncommon G1P[6] (21/72; 29.2%), and G2P[4] (9/72; 12.5%). Only two G-types were found: G1 (60/72; 83.3%) and G2 (11/72; 15.3%). Among the P-genotypes, P[8] was the most prevalent (34/72; 47.2%), followed by P[6] (22/72; 30.6%) and P[4] (9/72; 12.5%). In the phylogenetic trees, the identified G and P-types clustered tightly together and with reference sequences in specific monophyletic groups, with highly significant bootstrap values (≥92%).ConclusionThis pre-vaccination study revealed, for the first time for Bengo province (Angola), the RVA genotype profile, including phylogenetic relationships, and a high RVA detection rate, supporting the immediate introduction of a RVA vaccine in the national immunization programme.

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“…RVA studies in the elderly are uncommon since this virus is considered a major paediatric pathogen. 48 RVA detection rate in the enroled asymptomatic elderly (3.6%, 6 of 169) was in the range of the estimated for RVA causing endemic AGE in the adult population in different regions of the world (3%-5%). 17 The low number of positive samples impairs to envisage a clear infection seasonality, however, RVA was mostly detected during the winter and spring months, in agreement with other studies.…”

Section: Epidemiology Of Age Has Been Poorly Investigated In Portugalmentioning

confidence: 65%

“…RVA studies in the elderly are uncommon since this virus is considered a major paediatric pathogen . RVA detection rate in the enroled asymptomatic elderly (3.6%, 6 of 169) was in the range of the estimated for RVA causing endemic AGE in the adult population in different regions of the world (3%‐5%) .…”

Section: Discussionmentioning

confidence: 87%

Molecular epidemiology and host genetics of norovirus and rotavirus infections in Portuguese elderly living in aged care homes

Piedade

Nordgren

Esteves

et al. 2019

Journal of Medical Virology

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Norovirus (NoV) and rotavirus group A (RVA) are major agents of acute gastroenteritis worldwide. This study aimed to investigate their epidemiological profile in Portuguese elderly living in long‐term care facilities and to assess the host genetic factors mediating infection susceptibility. From November 2013 to June 2015, 636 faecal specimens from 169 elderly, mainly asymptomatic, living in nursing homes in Greater Lisbon and Faro district, Portugal, were collected. NoV and RVA were detected by real‐time polymerase chain reaction and NoV genotyped by phylogenetic analysis. NoV detection rate was 7.1% (12 of 169). Three GI.3 and one GII.6 strains were genotyped. RVA detection rate was 3.6% (6 of 169), exclusively in asymptomatic individuals. Host genetic factors associated with infection susceptibility were described on 250 samples by saliva‐based enzyme‐linked immunosorbent assays. The Lewis‐negative phenotype was 8.8% (22 of 250) and the rate of nonsecretors was 16.8% (42 of 250). Association to NoV and RVA infection was performed in the subgroup of individuals (n = 147) who delivered both faecal and saliva samples. The majority of NoV‐ and RVA‐positive individuals (90.9% and 83.3%, respectively) were secretor‐positive, with Lewis B phenotype. In a subset of individuals, FUT2 and FUT3 genes were genotyped to assess mutations and validate the secretor and Lewis phenotypes. All sequenced nonsecretors were homozygous for FUT2 nonsense mutation G428A. In this study, low detection rates of NoV and RVA infections were found during two winter seasons. However, even in the absence of any outbreak, the importance of finding these infections in a nonepidemic situation in long‐term care facilities may have important implications for infection control.

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High rate of detection of G8P[6] rotavirus in children with acute gastroenteritis in São Tomé and Príncipe

Cited by 12 publications

References 25 publications

Rotavirus and norovirus in children with severe diarrhea in Burkina Faso before rotavirus vaccine introduction

Rotavirus and norovirus in children with severe diarrhea in Burkina Faso before rotavirus vaccine introduction

Characterization of rotavirus infection in children with acute gastroenteritis in Bengo province, Northwestern Angola, prior to vaccine introduction

Molecular epidemiology and host genetics of norovirus and rotavirus infections in Portuguese elderly living in aged care homes

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