High Rates of Extensively Drug-Resistant Pseudomonas aeruginosa in Children with Cystic Fibrosis

Gutiérrez-Santana, Juan Carlos; Gerónimo-Gallegos, Armando; Martínez-Corona, Mónica B.; López-López, Marisol; Toscano-Garibay, Julia Dolores; Cuevas-Schacht, Francisco; Coria-Jiménez, Victor R.

doi:10.1007/s00284-022-03048-4

Cited by 5 publications

(3 citation statements)

References 30 publications

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“…[54,55] The frequency of MDR strains in these patients has increased from 15.7 % of MDR strains registered in the 2013 report of the CF Foundation (CFF) [56] to 42.4 % by 2022, coupled with 12.7 % of XDR variants. [57] Additionally, the lung environment of individuals with CF is crucial in the emergence and dispersal of P. aeruginosa variants with high transmission and propagation capacities, which have been defined as "epidemic strains" that have managed to infect and cause disease in individuals without CF and in their companion animals. [58]…”

Section: P Aeruginosa and People With Cfmentioning

confidence: 99%

“…The frequency of these variants tends to be higher in individuals with CF than in immunocompromised, burned or ulcerated patients, [53] and in different nations, variants resistant to carbapenems (CR) have recently been reported [54,55] . The frequency of MDR strains in these patients has increased from 15.7 % of MDR strains registered in the 2013 report of the CF Foundation (CFF) [56] to 42.4 % by 2022, coupled with 12.7 % of XDR variants [57] …”

Section: Pseudomonas Aeruginosamentioning

confidence: 99%

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Diagnosis and Therapeutic Strategies Based on Nucleic Acid Aptamers Selected against Pseudomonas aeruginosa: The Challenge of Cystic Fibrosis

Gutiérrez‐Santana,

Coria‐Jiménez

2023

ChemMedChem

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Antimicrobial resistance (AMR) is a rapidly spreading global health problem, and approximately five million deaths associated with AMR pathogens were identified prior to the COVID‐19 pandemic. Pseudomonas aeruginosa has developed increasing AMR, and in patients with cystic fibrosis (CF) colonized by this bacterium, rare phenotypes have emerged that complicate the diagnosis and treatment of the hosts, in addition to multiple associated “epidemic strains” with high morbidities and mortalities. The conjugation of aptamers with fluorochromes or nanostructures has allowed the design of new identification strategies for Pseudomonas aeruginosa with detection limits of up to 1 cell ⋅ mL−1, and the synergy of aptamers with antibiotics, antimicrobial peptides and nanostructures has exhibited promising therapeutic qualities. Some selected aptamers against this bacterium have shown intrinsic antimicrobial activity. However, these aptamers have been poorly evaluated in clinical isolates and have shown decreased interactions for CF isolates, demonstrating, in these cases, uncommon phenotypes resulting from the selective qualities of this disease as well as the great adaptive capacity of the pathogen. Therefore, finding an aptamer or set of aptamers that have the ability to recognize strange phenotypes of this bacillus is crucial in the battle against AMR.

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Section: P Aeruginosa and People With Cfmentioning

confidence: 99%

Section: Pseudomonas Aeruginosamentioning

confidence: 99%

Diagnosis and Therapeutic Strategies Based on Nucleic Acid Aptamers Selected against Pseudomonas aeruginosa: The Challenge of Cystic Fibrosis

Gutiérrez‐Santana,

Coria‐Jiménez

2023

ChemMedChem

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“…Antimicrobial activity assays of Clostrisin and Cellulosin were performed using microplate bioassays with strains from the American Type Culture Collection (ATCC): Staphylococcus aureus 43300 (MRSA), Pseudomonas aeruginosa ATCC 15442 (PA14), Escherichia coli IM08B as well as clinical strains isolated from patients in Mexico: Staphylococcus epidermidis MIQ43 (multidrug-resistant clinical sample) (internal code from the MicroIQ laboratory library), and Pseudomonas aeruginosa MIQPA25 [50] (multidrug-resistant clinical sample isolated from cystic fibrosis patients), and Clostridioides difficile R20291. All strains have a multidrug-resistant profile (Supplementary Table 5).…”

Section: Antimicrobial Activity Of Clostrisin and Cellulosinmentioning

confidence: 99%

Genome Mining Reveals the Structure of the Clostrisin and Cellulosin Biosynthetic Gene Clusters, Two Novel Lantibiotics

Alejo Hernandez,

Villavicencio Sánchez,

Sánchez Morales

et al. 2023

Preprint

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Antimicrobial resistance is an issue of enormous proportions that poses a significant threat to global public health. This study focuses on lanthipeptides, ribosomally encoded peptides which display significant structural diversity and hold promising potential as antibiotics. Genome mining was employed to locate biosynthetic gene clusters (BGC) for class II lanthipeptides. A phylogenetic study analyzing homologous sequences of functional LanM sequences revealed a unique evolutionary clade of 39 LanMs associated with 28 Clostridial bacterial genomes. In silico exploration identified nine complete BGCs, including one from Clostridium cellulovorans 743B, that harbors two new lanthipeptides: Clostrisin and Cellulosin. The lanthipeptide BGCs were heterologously expressed in Escherichia coli. Molecular weights associated with the expected post-translational modifications of the purified lanthipeptides were confirmed by MALDI-TOF. Both peptides demonstrated antimicrobial activity against multidrug-resistant bacteria, such as a clinical strain of Staphylococcus epidermidis MIQ43 and a reference strain of resistant Pseudomonas aeruginosa PA14. This study showcases the immense potential of genome mining in identifying new class II lanthipeptides.

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Prevalence of colistin resistance in clinical isolates of Pseudomonas aeruginosa: a systematic review and meta-analysis

Narimisa,

Keshtkar,

Dadgar-Zankbar

et al. 2024

Front. Microbiol.

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ObjectiveThe emergence of resistance to colistin, the last resort for treating severe infections caused by Pseudomonas aeruginosa, poses a significant threat to public health. This meta-analysis aimed to investigate the prevalence of colistin resistance in clinical isolates of P. aeruginosa.MethodA comprehensive search of MEDLINE (PubMed), Web of Science, and Scopus databases was conducted to identify relevant articles published until December 2023. Subsequently, a meta-analysis was performed using Stata software to examine the pooled prevalence of colistin resistance and to conduct subgroup analyses.ResultsA total of 619 studies were included in the meta-analysis, revealing a global prevalence of colistin resistance of 1% among all P. aeruginosa isolates. Furthermore, cystic fibrosis patients exhibited the highest resistance to colistin, with a prevalence of 7% among the examined diseases.ConclusionThe increase in colistin resistance in P. aeruginosa in recent years from 2% (in the period of 2006–2010) to 5% (in the period of 2020–2023) underscores the need for implementing infection prevention programs, using appropriate treatment regimens, and disseminating comprehensive information on antimicrobial resistance patterns. These measures are crucial for addressing this growing public health concern.

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High Rates of Extensively Drug-Resistant Pseudomonas aeruginosa in Children with Cystic Fibrosis

Cited by 5 publications

References 30 publications

Diagnosis and Therapeutic Strategies Based on Nucleic Acid Aptamers Selected against Pseudomonas aeruginosa: The Challenge of Cystic Fibrosis

Diagnosis and Therapeutic Strategies Based on Nucleic Acid Aptamers Selected against Pseudomonas aeruginosa: The Challenge of Cystic Fibrosis

Genome Mining Reveals the Structure of the Clostrisin and Cellulosin Biosynthetic Gene Clusters, Two Novel Lantibiotics

Prevalence of colistin resistance in clinical isolates of Pseudomonas aeruginosa: a systematic review and meta-analysis

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