High Resistance to Cucumber Mosaic Virus Conferred by Satellite RNA and Coat Protein in Transgenic Commercial Tobacco Cultivar G-140

Y, Yie; Zhao, Fengyuan; Sz, Zhao; Yz, Liu; Yl, Liu; Tien, Phat

doi:10.1094/mpmi-5-460

Cited by 39 publications

(14 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although this may alter the vector relations and spread of a virus in a CPtransgenic monoculture or even the host range of a virus (if a different vector species became involved), the effect would apply only for a single acquisitiontransmission cycle. Any long-term, stable effects, genetic or epidemiological, would seem remote-but are now amenable to direct experimentation and more accurate risk assessment using the exten-sive range of plant species transformed singly or combinatorially (132)(133)(134) (132,133) and, in general, exhibits greater susceptibility to virus challenge, probably through added environmental stresses. Nevertheless, useful virus-resistant lines can be selected (133).…”

Section: The Final Word-on Risk Issues Concerned Withmentioning

confidence: 99%

Strategies to protect crop plants against viruses: pathogen-derived resistance blossoms.

Wilson¹

1993

Proc. Natl. Acad. Sci. U.S.A.

230

View full text Add to dashboard Cite

Since 1986, the ability to confer resistance against an otherwise devastating virus by introducing a single pathogen-derived or virus-targeted sequence into the DNA of a potential host plant has had a marked influence on much of the research effort, focus, and shortterm objectives of plant virologists throughout the world. The vast literature on coat protein-mediated protection, for example, attests to our fascination for unraveling fundamental molecular mechanism(s), our (vain) Despite extensive and sometimes elegant experimentation, the molecular mechanism(s) of this viral "crossprotection" have remained elusive and controversial. In some cases, the coat protein (CP) of the protectant virus was thought to be primarily responsible, either by preventing particle disassembly or by re-encapsidating the incoming genome of the more severe challenge virus. However, viroids [240-to 380-nt-long, naked circular single-stranded (ss)RNA pathogens] and mutant viruses making assembly-defective or no detectable CP could also cross-protect against their more severe relatives. Such observations prompted models based on inhibitory interactions between sense and antisense RNAs or between the replicational machineries ofthe two competing pathogens (6). Controversy arose largely because available molecular technology could not resolve which regulatory or coding sequence(s) or polypeptide product(s) from the actively replicating genome of the primary (protectant) pathogen were responsible for interfering with the many replicative processes essential to establish infection by the secondary, related, and more severe virus. In contrast, multiple infections by unrelated viruses sharing a common host are very prevalent in nature. Many aspects of this older story have their parallels in current hypotheses and lack of a unified model for pathogenderived resistance in transgenic plants.The advent of improved cell and tissue culture techniques, efficient protocols for Agrobacterium tumefaciens-mediated transformation and plantlet regeneration in dicotyledonous species (7) [and more recent methods for monocot crops (8-10)], has permitted, among other applications (11,12), the theory of pathogenderived virus resistance to be tested in practice.Collaboration between researchers at Monsanto and Washington University (St. Louis) led to the first report of CPmediated protection (CPMP) against tobacco mosaic virus (TMV) in tobacco in 1986 (4). Since then, CPMP has been reported for over 20 viruses in at least 10 different taxonomic groups, in a wide 3134 variety of dicot plant species, and the list is increasing rapidly.

show abstract

Section: The Final Word-on Risk Issues Concerned Withmentioning

confidence: 99%

Strategies to protect crop plants against viruses: pathogen-derived resistance blossoms.

Wilson¹

1993

Proc. Natl. Acad. Sci. U.S.A.

230

View full text Add to dashboard Cite

show abstract

“…The various reports on CPMR, however, indicate very differently, and sometimes even opposing outcomes, which might be partly due to different levels of CP accumulation obtained on account of different gene constructs and/or different host systems. For all reported successful cases of CP-mediated resistance against CMV, high expression levels of transgenic CP were reached (Cuozzo et al 1988;Quemada et al 1991;Gonsalves et al 1992Gonsalves et al , 1994Namba et al 1992;Yie et al 1992;Nakajima et al 1993;Yoshioka et al 1993;Okuno et al 1993a;Kaniewski et al 1999;Jacquemond et al 2001;Shin et al 2002b), although it is known that this did not at all assure resistant phenotype (Jacquemond et al 2001). The resistance responses in the different CMV-host combinations seems to vary considerably, in some cases only a temporary delay in symptom appearance was observed.…”

Section: Discussionmentioning

confidence: 99%

Expression of coat protein gene ofCucumber mosaic virus(CMV-subgroup IA)Gladiolusisolate inNicotiana tabacum

Dubey

Chandrasekhar

Srivastava³

et al. 2015

Journal of Plant Interactions

View full text Add to dashboard Cite

The coat protein (CP)-mediated resistance against Cucumber mosaic virus (CMV) subgroup IA was developed in transgenic lines of Nicotiana tabacum cv. Petit Havana using Agrobacterium tumefaciens-mediated transformation. Ten independently transformed lines have developed, four of which were tested for resistance against CMV using virus challenge inoculations. The transgenic lines exhibiting complete resistance remained healthy and symptomless in their life span and showed reduced or no virus accumulation in their systemic leaves after virus challenge inoculation. These transgenic lines also showed resistance against CMV strains which are not closely related to CMV-Gladiolus strains. This is the first report of CP-mediated transgenic resistance against a CMV subgroup IA member isolated from India showing resistance to all CMV strains occurring in the same vicinity.

show abstract

“…Alongside these major developments in plant virus genomederived transgenes there has been sustained interest in other virus-dependent (parasitic) RNA or DNA sequences with the capacity to ameliorate symptoms and/or to cause reduced virus replication and to increase plant tolerance, resistance or even immunity. Specifically these include satellite RNAs Gerlach et al, 1987;Ponz et al, 1987;Jacquemond et al, 1988;Roossinck et al, 1992;McGarvey et al, 1994;Kurath and Dodds, 1994), defective interfering (DI) RNAs (Hillman et al, 1987;Li et al, 1989;Burgyan et al, 1989;Jones et al, 1990;Marsh et al, 1991a, b;De Oliveira Resende et al, 1991Yie et al, 1992;Yie and Tien, 1993;White and Morris, 1994) or DNAs (Stanley et al, 1990;Frischmuth and Stanley, 1991;Stenger,1994) and ribozyme (Uhlenbeck, 1987;Haseloff and Gerlach, 1988;Symons, 1991;Mazzolini et al, 1992;Edington et al, 1992;Steinecke et al, 1992;L'Huillier et al, 1992) sequences connected to viral antisense arm sequences (10-20 nucleotides) for binding to a target site.…”

Section: Pathogen-derived Resistance Strategies In Crop Protectionmentioning

confidence: 99%

Pathogen-Derived Resistance Against Plant Viruses: Postscript and Prospects

Arif¹,

Hassan²

1999

Pakistan J. of Biological Sciences

View full text Add to dashboard Cite

Pathogen-derived resistance strategies using coat proteins, movement proteins, non-structural proteins. replicases, antisense and satellite RNAs for the production of virus-resistant transgenic plants are briefly discussed. The concept of recombination of viral RNA with transgene products is critically analyzed and potential risks associated with commercialization of transgenic plants with viral inserts and its effects on bio-safety, are highlighted.

show abstract

High Resistance to Cucumber Mosaic Virus Conferred by Satellite RNA and Coat Protein in Transgenic Commercial Tobacco Cultivar G-140

Cited by 39 publications

References 0 publications

Strategies to protect crop plants against viruses: pathogen-derived resistance blossoms.

Strategies to protect crop plants against viruses: pathogen-derived resistance blossoms.

Expression of coat protein gene ofCucumber mosaic virus(CMV-subgroup IA)Gladiolusisolate inNicotiana tabacum

Pathogen-Derived Resistance Against Plant Viruses: Postscript and Prospects

Contact Info

Product

Resources

About