High Resistivity Lipid Bilayers Assembled on Polyelectrolyte Multilayer Cushions: An Impedance Study

Diamanti, Eleftheria; Gregurec, Danijela; Rodríguez-Presa, María José; Gervasi, C.A.; Azzaroni, Omar; Moya, Sergio

doi:10.1021/acs.langmuir.6b01191

Cited by 24 publications

(21 citation statements)

References 42 publications

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“…[5] In particular, fused deposition modeling (FDM) allows the manufacturing of objects by the to minimize the amount of material used. [22,23] LbL has been widely used in the biomedical sector, including substrates with tunable properties for theranostics [24] or the design of micropatterned protein arrays. [25] LbL has exploited the use of polysaccharides to produce biodegradable LbL build-ups from bio-based materials.…”

Section: Introductionmentioning

confidence: 99%

“…[ 18–20 ] This technology is known since 1997 [ 21 ] and consists in the fabrication of thin films via electrostatic or supramolecular interactions of different polymers, which allows to minimize the amount of material used. [ 22,23 ] LbL has been widely used in the biomedical sector, including substrates with tunable properties for theranostics [ 24 ] or the design of micropatterned protein arrays. [ 25 ]…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Design of a Bio‐Based Device for Micro Total Analysis Combining Fused Deposition Modeling and Layer‐by‐Layer Technologies

León

Frutos

Molina

2020

Macro Materials & Eng

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Compared to other fabrication methods such as micromolding [7] or soft lithography, [8] objects manufactured via FDM are produced in their final shape without any external template, additional tools, or further postprocessing. Since FDM does not require any mastermold, it allows the fabrication of complex, tailorable lattices with high reproducibility, such as highly hollow or gyroidal structures. [9] FDM feedstock is commodity plastics, avoiding the use of hazard or expensive compounds. Moreover, FDM printers are more economic and relatively easy to use than most of other (micro)fabrication equipment. The printed objects are previously designed using a software and all the information needed for fabrication is contained within a computer assisted design file. These designs can be easily modified to meet the quick changing needs of the market, allowing high flexibility in the product and reducing the product development process. [10] Since 3D printing technologies emerged, polylactic acid (PLA) has become one of the most widely used materials in the FDM technology due to its cost, biodegradability, and good mechanical properties. [11] PLA can be manufactured from natural sources as sugarcane or corn starch and possesses mechanical properties comparable to fossil-fuel derived polystyrene. [12] Among its limitations, it presents a low maximum service temperature (≈60 °C), which narrows its use in engineering applications. [13] However, it can be used as structural matrix when working at room or human body temperature. Bio-based polymers, including PLA, have been printed to fabricate tablets, [14] scaffolds, [15] or even organs individually adapted for each patient. [16] In addition, since additive manufacturing techniques emerged, there is a growing number of initiatives to fabricate different equipment and laboratory supplies in house, [17] which would allow reducing the production chain, saving time and money. Another important factor to consider in the design of micro total analysis systems is the surface modification of the material so it can selectively adsorb the target biomolecule. Among other alternatives, the LbL technology is an interesting option which allows the modification of large surfaces in an inexpensive manner. [18-20] This technology is known since 1997 [21] and consists in the fabrication of thin films via electrostatic or supramolecular interactions of different polymers, which allows In this paper a methodology is presented to fabricate a micropatterned device made of bio-based polymers (polylactic acid, sodium alginate (ALG), and chitosan (CHI)) suitable for micro total analysis. Fused deposition modeling and layer-by-layer technologies are combined to create tailorable devices with submillimetric controlled well size that can be rapidly prepared in house. As proof of concept, the influence of immobilization of ALG/CHI bilayers in the selective adsorption of alkaline phosphatase and its activity is studied. Due to the noncovalent nature of these interactions, it is proven that these devices ...

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Design of a Bio‐Based Device for Micro Total Analysis Combining Fused Deposition Modeling and Layer‐by‐Layer Technologies

León

Frutos

Molina

2020

Macro Materials & Eng

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“…26,36 Electrochemical impedance spectroscopy (EIS) is used to measure the complex impedance properties of a material and has been widely used to study interfacial interactions, such as antigen-antibody interaction, and cancer cell capture as well as for evaluation of biomembranes. 27,[37][38][39] EIS was implemented here at gold microcavity array substrates analogous in structure to the optically transparent PDMS arrays used for FCS. The combination of FLCS and EIS provides new insights into the dynamics of the membrane and impact of PP50 polymer on lipid membrane lateral packing and integrity in real time.…”

Section: Introductionmentioning

confidence: 99%

Dynamic studies of the interaction of a pH responsive, amphiphilic polymer with a DOPC lipid membrane

et al. 2017

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Deeper understanding of the molecular interactions between polymeric materials and the lipid membrane is important across a range of applications from permeation for drug delivery to encapsulation for immuno-evasion. Using highly fluidic microcavity supported lipid bilayers, we studied the interactions between amphiphilic polymer PP50 and a DOPC lipid bilayer. As the PP50 polymer is pH responsive the studies were carried out at pH 6.5, 7.05 and 7.5, corresponding to fully, partly protonated (pH = pK = 7.05) and fully ionized states of the polymer, respectively. Fluorescence correlation spectroscopy (FCS) using both labelled lipid and polymer revealed the PP50 associates with the bilayer interface across all pHs where its diffusion along the interface is impeded. Both FCS and electrochemical impedance spectroscopy (EIS) data indicate that the PP50 does not penetrate fully into the bilayer core but rather forms a layer at the bilayer aqueous interface reflected in increased resistance and decreased capacitance of the bilayer on PP50 binding. The extent of these effects and the dynamics of binding are influenced by pH, increasing with decreasing pH. These experimental trends concurred with coarse grained Monte Carlo simulations of polymer-bilayer interactions wherein a model hydrophilic polymer backbone grafted with side chains of varying hydrophobicity, to mimic the effect of varying pH, was simulated based on the bond fluctuation model with explicit solvent. Simulation results showed that with increasing hydrophobicity, the polymer penetrated deeper into the contacting bilayer leaflet of the membrane suppressing, consistent with EIS data, solvent permeation and that a full insertion of the polymer into the bilayer core is not necessary for suppression of permeability.

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“…Bilayer formation takes place if the molar percentage of DOPS lies between 50 and 70%. Electrochemical impedance spectroscopy (EIS) studies performed by Diamanti et al 24 showed that the lipid bilayer assembled on PAH/PSS displayed high resistance values: 1.89 Â 10 7 O cm 2 . This resistance is comparable with the resistance of a black lipid membrane and thus allows the introduction of channels or trans-membrane proteins for selective transport.…”

Section: Introductionmentioning

confidence: 99%

Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: an electrochemical impedance study

et al. 2017

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Supported membranes on polymer cushions are of fundamental interest as models for cell membranes. The use of polyelectrolyte multilayers (PEMs) assembled by the layer by layer (LbL) technique as supports for a bilayer allows for easy integration of the lipid bilayer on surfaces and devices and for nanoscale tunable spacing of the lipid bilayer. Controlling ionic permeability in lipid bilayers supported on PEMs triggers potential applications in sensing and as models for transport phenomena in cell membranes. Lipid bilayers displaying gramicidin channels are fabricated on top of polyallylamine hydrochloride (PAH) and polystyrene sulfonate (PSS) multilayer films, by the assembly of vesicles of phosphatidylcholine and phosphatidylserine, 50 : 50 M/M, carrying gramicidin (GA). Quartz crystal microbalance with dissipation shows that the vesicles with GA fuse into a bilayer. Atomic force microscopy reveals that the presence of GA alters the bilayer topography resulting in depressions in the bilayer of around 70 nm in diameter. Electrochemical impedance spectroscopy (EIS) studies show that supported bilayers carrying GA have smaller resistances than the bilayers without GA. Lipid layers carrying GA display a higher conductance for K than for Na and are blocked in the presence of Ca.

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High Resistivity Lipid Bilayers Assembled on Polyelectrolyte Multilayer Cushions: An Impedance Study

Cited by 24 publications

References 42 publications

Design of a Bio‐Based Device for Micro Total Analysis Combining Fused Deposition Modeling and Layer‐by‐Layer Technologies

Design of a Bio‐Based Device for Micro Total Analysis Combining Fused Deposition Modeling and Layer‐by‐Layer Technologies

Dynamic studies of the interaction of a pH responsive, amphiphilic polymer with a DOPC lipid membrane

Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: an electrochemical impedance study

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