High-resolution 1H nuclear magnetic resonance spectroscopy analysis of bile samples obtained from a patient after orthotopic liver transplantation: new perspectives

Paczkowska, A; Toczyłowska, Beata; Nyckowski, P; Patkowski, Waldemar; Kański, Andrzej; Krawczyk, Marek; Ołdakowska‐Jedynak, Urszula

doi:10.1016/s0041-1345(03)00788-7

Cited by 6 publications

(3 citation statements)

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“…7,25 These studies investigated the bile collected from the gall bladder, which is easier to obtain and much more concentrated than hepatic bile, and most studies to date on liver transplantation monitoring have used gall bladder bile from the graft recipient. 26 However, hepatic bile can give more direct information on the excretory function of the liver. This has been shown in liver transplantation studies where NMR spectroscopy of hepatic bile provided important information regarding graft viability.…”

Section: Introductionmentioning

confidence: 99%

Identification of metabolites in human hepatic bile using 800 MHz 1H NMR spectroscopy , HPLC-NMR/MS and UPLC-MS

et al. 2009

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The first application of high field NMR spectroscopy (800 MHz for (1)H observation) to human hepatic bile (as opposed to gall bladder bile) is reported. The bile sample used for detailed investigation was from a donor liver with mild fat infiltration, collected during organ retrieval prior to transplantation. In addition, to focus on the detection of bile acids in particular, a bile extract was analysed by 800 MHz (1)H NMR spectroscopy, HPLC-NMR/MS and UPLC-MS. In the whole bile sample, 40 compounds have been assigned with the aid of two-dimensional (1)H-(1)H TOCSY and (1)H-(13)C HSQC spectra. These include phosphatidylcholine, 14 amino acids, 10 organic acids, 4 carbohydrates and polyols (glucose, glucuronate, glycerol and myo-inositol), choline, phosphocholine, betaine, trimethylamine-N-oxide and other small molecules. An initial NMR-based assessment of the concentration range of some key metabolites has been made. Some observed chemical shifts differ from expected database values, probably due to a difference in bulk diamagnetic susceptibility. The NMR spectra of the whole extract gave identification of the major bile acids (cholic, deoxycholic and chenodeoxycholic), but the glycine and taurine conjugates of a given bile acid could not be distinguished. However, this was achieved by HPLC-NMR/MS, which enabled the separation and identification of ten conjugated bile acids with relative abundances varying from approximately 0.1% (taurolithocholic acid) to 34.0% (glycocholic acid), of which, only the five most abundant acids could be detected by NMR, including the isomers glycodeoxycholic acid and glycochenodeoxycholic acid, which are difficult to distinguish by conventional LC-MS analysis. In a separate experiment, the use of UPLC-MS allowed the detection and identification of 13 bile acids. This work has shown the complementary potential of NMR spectroscopy, MS and hyphenated NMR/MS for elucidating the complex metabolic profile of human hepatic bile. This will be useful baseline information in ongoing studies of liver excretory function and organ transplantation.

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Section: Introductionmentioning

confidence: 99%

Identification of metabolites in human hepatic bile using 800 MHz 1H NMR spectroscopy , HPLC-NMR/MS and UPLC-MS

et al. 2009

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“…Combined with advanced statistical approaches, 1 H-NMR spectroscopy measures metabolic profiles that characterize changes of specific organ functions. Referred to as "metabolomics" or "metabonomics" (5, 6), this analytic technique has identified a number of novel metabolic biomarkers for the early detection of renal donor graft dysfunction (6 -9), but have yet to be systematically tested in hepatic injury that results in graft failure (10,11).In this case report, NMR analysis was applied to identify metabolic markers of graft dysfunction in a patient who suffered graft failure after the first orthotopic liver transplantation (OLTx) from a living-related donor followed by a second successful OLTx from a deceased donor. Hence, the patient served as his own control for comparing metabolic profiles before and after transplantation.…”

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Early Detection of Graft Failure Using the Blood Metabolic Profile of a Liver Recipient

et al. 2007

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In this case report we describe the blood metabolic profile ("metabolomics") by nuclear magnetic resonance (NMR) spectroscopy and principle component analysis (PCA) from a patient who underwent two consecutive liver transplantations. The first graft from a living-related donor failed and was followed by a second successful transplant from a deceased donor. Using quantitative high-resolution H-NMR spectroscopy, 48 endogenous metabolites were analyzed in whole blood samples at baseline and different time points after each transplantation. From 48 analyzed metabolites, six metabolites were identified by PCA as metabolic markers consistent with a non-functional liver after first transplantation. Importantly, this distinctive metabolic profile was present as early as two hours after first transplant surgery when no other variable or conventional laboratory tests indicated poor graft function. This article reports the potential usefulness of quantitative H-NMR based metabolomics to diagnose early graft dysfunction in liver transplantation.

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Pattern recognition and biomarker validation using quantitative¹H-NMR-based metabolomics

Serkova

Niemann

2006

Expert Review of Molecular Diagnostics

149

123

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The collection of global metabolic data and their interpretation (both spectral and biochemical) using modern spectroscopic techniques and appropriate statistical approaches, are known as 'metabolic profiling', 'metabonomics' or 'metabolomics'. This review addresses 1H-nuclear magnetic resonance (NMR)-based metabolomic principles and their application in biomedical science, with special emphasis on their potential in translational research in transplantation, oncology, and drug toxicity or discovery. Various steps in metabolomics analysis are described in order to illustrate the types of biological samples, their respective handling and preparation for 1H-NMR analysis; provide a rationale for using pattern-recognition techniques (spectral database concept) versus quantitative 1H-NMR-based metabolomics (metabolite database concept); and identify necessary technological and logistical future developments that will allow 1H-NMR-based metabolomics to become an established tool in biomedical research and patient care.

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High-resolution 1H nuclear magnetic resonance spectroscopy analysis of bile samples obtained from a patient after orthotopic liver transplantation: new perspectives

Cited by 6 publications

References 6 publications

Identification of metabolites in human hepatic bile using 800 MHz 1H NMR spectroscopy , HPLC-NMR/MS and UPLC-MS

Identification of metabolites in human hepatic bile using 800 MHz 1H NMR spectroscopy , HPLC-NMR/MS and UPLC-MS

Early Detection of Graft Failure Using the Blood Metabolic Profile of a Liver Recipient

Pattern recognition and biomarker validation using quantitative¹H-NMR-based metabolomics

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High-resolution 1H nuclear magnetic resonance spectroscopy analysis of bile samples obtained from a patient after orthotopic liver transplantation: new perspectives

Cited by 6 publications

References 6 publications

Identification of metabolites in human hepatic bile using 800 MHz 1H NMR spectroscopy , HPLC-NMR/MS and UPLC-MS

Identification of metabolites in human hepatic bile using 800 MHz 1H NMR spectroscopy , HPLC-NMR/MS and UPLC-MS

Early Detection of Graft Failure Using the Blood Metabolic Profile of a Liver Recipient

Pattern recognition and biomarker validation using quantitative1H-NMR-based metabolomics

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Pattern recognition and biomarker validation using quantitative¹H-NMR-based metabolomics