High‐Resolution 4DImaging in Live Cells

Abstract

The technological sophistication of microscopy approaches has grown by leaps and bounds over the past decade. This has yielded microscopes capable of greater sensitivity, faster acquisition, and higher resolution, as well as fluorophores and imaging tools that demonstrate more and more diversity of properties and applications. Yet for all the putative improvements to the cell biologists' tool kit, the application of potentially invaluable tools to unsolved scientific problems is still in its infancy. The purpose of our review is to discuss important innovations in live‐cell imaging, focusing on practical considerations for probing biological systems at the molecular level while remaining rooted in a live‐cell context.