High resolution/accurate mass (HRMS) detection of anatoxin-a in lake water using LDTD–APCI coupled to a Q-Exactive mass spectrometer

Roy-Lachapelle, Audrey; Solliec, Morgan; Sinotte, Marc; Deblois, Christian; Sauvé, Sébastien

doi:10.1016/j.talanta.2014.10.021

Cited by 27 publications

(24 citation statements)

References 29 publications

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“…For the analysis of ANA-a via the LDTD-APCI-HRMS method and of MCs and ANA-a via the UHPLC-HESI-HRMS system, the weighed samples were mixed with 5 mL of acidified MeOH (formic acid, pH 2) in polyethylene conical-bottom centrifuge tubes [27,31]. The samples were homogenized for 10 min using ultrasonication and then they were submitted to centrifugation at 3500 rpm for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…The precursor ions are filtered by the quadrupole which operates at an isolation width of 0.4 amu. A resolving power of 17,500 FWHM at m / z 200 was used with an automatic gain control (AGC) target set at 1 × 10 5 ions for a maximum injection time of 50 ms. For more technical details, please refer to these previous studies [27,65,66,67]. …”

Section: Methodsmentioning

confidence: 99%

“…The structural identification and compound profiles were obtained using the Q-Exactive orbital ion trap, which enabled selective detection for all targeted compounds, both with and without the use of certified standard materials. For this purpose, we used different analytical approaches previously developed in our laboratory including laser diode thermal desorption (LDTD) for a fast screening of total MCs and ANA-a (approximately 10 s per sample) while eliminating a chromatographic separation step [27,28,29]. More specifically, Lemieux oxidation using potassium permanganate and sodium periodate allowed the recovery of the 2-methyl-3-methoxy-4-phenylbutyric acid (MMPB) moiety common to all MCs which includes over 100 known congeners [30].…”

Section: Introductionmentioning

confidence: 99%

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Detection of Cyanotoxins in Algae Dietary Supplements

Roy-Lachapelle

Solliec

Bouchard

et al. 2017

Toxins

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112

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Algae dietary supplements are marketed worldwide as natural health products. Although their proprieties have been claimed as beneficial to improve overall health, there have been several previous reports of contamination by cyanotoxins. These products generally contain non-toxic cyanobacteria, but the methods of cultivation in natural waters without appropriate quality controls allow contamination by toxin producer species present in the natural environment. In this study, we investigated the presence of total microcystins, seven individual microcystins (RR, YR, LR, LA, LY, LW, LF), anatoxin-a, dihydroanatoxin-a, epoxyanatoxin-a, cylindrospermopsin, saxitoxin, and β-methylamino-l-alanine in 18 different commercially available products containing Spirulina or Aphanizomenon flos-aquae. Total microcystins analysis was accomplished using a Lemieux oxidation and a chemical derivatization using dansyl chloride was needed for the simultaneous analysis of cylindrospermopsin, saxitoxin, and β-methylamino-l-alanine. Moreover, the use of laser diode thermal desorption (LDTD) and ultra-high performance liquid chromatography (UHPLC) both coupled to high resolution mass spectrometry (HRMS) enabled high performance detection and quantitation. Out of the 18 products analyzed, 8 contained some cyanotoxins at levels exceeding the tolerable daily intake values. The presence of cyanotoxins in these algal dietary supplements reinforces the need for a better quality control as well as consumer’s awareness on the potential risks associated with the consumption of these supplements.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Detection of Cyanotoxins in Algae Dietary Supplements

Roy-Lachapelle

Solliec

Bouchard

et al. 2017

Toxins

Self Cite

112

View full text Add to dashboard Cite

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“…One effective opportunity to realize the sampling is direct laser desorption (LD) that does not require additional media other than electricity. It is commonly used as the desorption step for various applications such as investigation of biological tissues [19][20][21], aerosols [22], pesticides [23], drugs [24], and explosives [25,26]. Drugs and explosives are relevant classes in forensic science.…”

Section: Introductionmentioning

confidence: 99%

Ambient Pressure Laser Desorption—Chemical Ionization Mass Spectrometry for Fast and Reliable Detection of Explosives, Drugs, and Their Precursors

et al. 2018

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Fast and reliable information is crucial for first responders to draw correct conclusions at crime scenes. An ambient pressure laser desorption (APLD) mass spectrometer is introduced for this scenario, which enables detecting substances on surfaces without sample pretreatment. It is especially useful for substances with low vapor pressure and thermolabile ones. The APLD allows for the separation of desorption and ionization into two steps and, therefore, both can be optimized separately. Within this work, an improved version of the developed system is shown that achieves limits of detection (LOD) down to 500 pg while remaining fast and flexible. Furthermore, realistic scenarios are applied to prove the usability of this system in real-world issues. For this purpose, post-blast residues of a bomb from the Second World War were analyzed, and the presence of PETN was proven without sample pretreatment. In addition, the analyzable substance range could be expanded by various drugs and drug precursors. Thus, the presented instrumentation can be utilized for an increased number of forensically important compound classes without changing the setup. Drug precursors revealed a LOD ranging from 6 to 100 ng. Drugs such as cocaine hydrochloride, heroin, (3,4-methylendioxy-methamphetamine) hydrochloride (MDMA) hydrochloride, and others exhibit a LOD between 10 to 200 ng.

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“…In particular, the combination of liquid chromatography and a quadrupole-based mass spectrometer operated in the selected reaction monitoring mode (SRM) is widely used to monitor levels of toxins in complex matrices [10, 17, 43–49]. Alternatively to the quadrupole-based techniques, two hybrid high resolution mass spectrometry systems have been used for qualitative detection and identification of cyanobacterial toxins in environmental samples: (a) Fourier transform (FT)-based mass spectrometers such as the linear ion trap (LTQ)-orbitraps [50, 51] and LTQ-FT instruments [18, 52], and (b) orthogonal acceleration hybrid quadrupole time-of-flight (oa Q-TOF) mass spectrometers [46, 53]. The advantages of high resolution instruments in environmental toxin monitoring are considered as superior accuracy and reliability[54].…”

Section: Introductionmentioning

confidence: 99%

Integrated Identification and Quantification of Cyanobacterial Toxins from Pacific Northwest Freshwaters by Liquid Chromatography and High-resolution Mass Spectrometry

et al. 2018

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The occurrence of harmful algal blooms in nutrient-rich freshwater bodies has increased world-wide, including in the Pacific Northwest. Some cyanobacterial genera have the potential to produce secondary metabolites that are highly toxic to humans, livestock and wildlife. Reliable methods for the detection of cyanobacterial toxins with high specificity and low limits of detection are in high demand. Here we test a relatively new hybrid high resolution accurate mass quadrupole time-of-flight mass spectrometry platform (TripleTOF) for the analysis of cyanobacterial toxins in freshwater samples. We developed a new method that allows the quantitative analysis of four commonly observed microcystin congeners (LR, LA, YR, and RR) and anatoxin-a in a 6-min LC run without solid-phase enrichment. Limits of detection for the microcystin congeners (LR, LA, YR, and RR) and anatoxin-a were <5 ng/L (200-fold lower than the guideline value of 1 μg/L as maximum allowable concentration of MC-LR in drinking water). The method was applied for screening freshwaters in the Pacific Northwest during the bloom and post-bloom periods. The use of high resolution mass spectrometry and concomitant high sensitivity detection of specific fragment ions with high mass accuracy provides an integrated approach for the simultaneous identification and quantification of cyanobacterial toxins. The method is sensitive enough for detecting the toxins in single Microcystis colonies.

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High resolution/accurate mass (HRMS) detection of anatoxin-a in lake water using LDTD–APCI coupled to a Q-Exactive mass spectrometer

Cited by 27 publications

References 29 publications

Detection of Cyanotoxins in Algae Dietary Supplements

Detection of Cyanotoxins in Algae Dietary Supplements

Ambient Pressure Laser Desorption—Chemical Ionization Mass Spectrometry for Fast and Reliable Detection of Explosives, Drugs, and Their Precursors

Integrated Identification and Quantification of Cyanobacterial Toxins from Pacific Northwest Freshwaters by Liquid Chromatography and High-resolution Mass Spectrometry

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