High resolution acrylamide gel electrophoresis of histones

Panyim, Sakol; Chalkley, Roger

doi:10.1016/0003-9861(69)90042-3

Cited by 2,439 publications

(624 citation statements)

References 22 publications

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“…The treatment resulted in a substantial, greater than 80%, inhibition of HBV gene expression and replication in vitro. 7 However, in this model system, tandem copies of the HBV genome had been integrated into the host genome. This situation, though challenging from a treatment point of view, is different from the usual situation in humans in which nonintegrated HBV genomes are responsible for production of new virus.…”

Section: Discussionmentioning

confidence: 99%

“…Lysates were spun in an Eppendorf centrifuge, ical acid-urea gel electrophoresis. 7 The purified conjugate was dialyzed against 0.025 mol/L Tris, 0.001 mol/L ethylenedi-and supernatants recovered. Ten micrograms of total supernatant protein was applied on a sodium dodecyl sulfate polyaminetetraacetic acid (EDTA) through membranes with 12-to 14-kd exclusion limits.…”

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confidence: 99%

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Inhibition of Hepatitis B Virus Replication by Targeted Pretreatment of Complexed Antisense Dna In Vitro

Nakazono

Ito

et al. 1996

Hepatology

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HBV surface antigen (HBsAg) accumulation was in-In the current study, we sought to determine hibited by 97%. The inhibition lasted for 6 days and whether targeted pretreatment of hepatocytes with was dose dependent. Controls consisting of antisense antisense DNA could prevent expression and replicaalone and a random oligo complex showed no signifi-tion of HBV when host cells were subsequently cant effect on any of the parameters under identical transfected with a large amount of HBV DNA. gel electrophoresis stained with ethidium bromide.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Inhibition of Hepatitis B Virus Replication by Targeted Pretreatment of Complexed Antisense Dna In Vitro

Nakazono

Ito

et al. 1996

Hepatology

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“…The association of H1" gene expression and terminal cell differentiation [Panyim and Chalkley, 1969;Gjerset et al, 1982;Alonso et al, 1988;Cheng and Skovetchi, 19891 is reflected in a very complex promoter structure. In addition to the TATA box, the H1 box, and an Spl site (see above), the H1" promoter of mouse and man contains a binding site for the retinoic acid receptor [Breuer et al, 1989[Breuer et al, , 1993Bouterfa et al, in press] and other conserved sequence motifs within otherwise divergent promoter sequences.…”

Section: H14mentioning

confidence: 99%

Organization and expression of H1 histone and H1 replacement histone genes

Doenecke

Albig

Bouterfa

et al. 1994

J of Cellular Biochemistry

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The H I family is the most divergent subgroup of the highly conserved class of histone proteins [Cole: Int J Pept Protein Res 30:433-449, 19871. In several vertebrate species, the H I complement comprises five or more subtypes, and tissue specific patterns of H I histones have been described. The diversity of the H I histone family raises questions about the functions of different H I subtypes and about the differential control of expression of their genes. The expression of main type H I genes is coordinated with DNA replication, whereas the regulation of synthesis of replacement H I subtypes, such as H I " and H5, and the testis specific H I t appears to be more complex. The differential control of H I gene expression is reflected in the chromosomal organization of the genes and in different promoter structures. This review concentrates on a comparison of the chromosomal organization of main type and replacement H I histone genes and on the differential regulation of their expression. General structural and functional data, which apply to both H I and core histone genes and which are covered by recent reviews, will not be discussed in detail.1994 WtIey-Liss, Inc

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“…Acid-urea polyacrylamide gel electrophoresis was performed with a 15% gel column (0.6 x 7 cm) containing 0.9 M acetic acid and 2.5 M urea as in [13]. After electrophoresis, the gel was stained with 0.2% Coomassie brilliant blue in 9.2% acetic acid and 45.4% methanol (v/v), destained, then dried.…”

Section: Gel Electrophoresismentioning

confidence: 99%

ADP‐ribosylation regulates the phosphorylation of histones by the catalytic subunit of cyclic AMP‐dependent protein kinase

et al. 1983

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Phosphorylation of whole histones from calf thymus by the catalytic subunit of cyclic AMP-dependent protein kinase was markedly reduced when the histones were ADP-ribosylated. NAD, nicotinamide or free ADP-ribose molecule did not suppress the phosphorylation.Urea gel electrophoretic analyses of the phosphorylated histones which had already been ADP-ribosylated revealed that the suppression of phosphorylation occurred in both Hl and core histones. Therefore, the possibility that ADP-ribosylation may regulate the phosphorylation of histones phosphorylation in nuclei warrants further investigation. MonofADP-ribosyl)ationADP-ribosyltransferase Pho.sphoryiation NAD CAMP-dependent protein kinase Histone

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High resolution acrylamide gel electrophoresis of histones

Cited by 2,439 publications

References 22 publications

Inhibition of Hepatitis B Virus Replication by Targeted Pretreatment of Complexed Antisense Dna In Vitro

Inhibition of Hepatitis B Virus Replication by Targeted Pretreatment of Complexed Antisense Dna In Vitro

Organization and expression of H1 histone and H1 replacement histone genes

ADP‐ribosylation regulates the phosphorylation of histones by the catalytic subunit of cyclic AMP‐dependent protein kinase

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