High‐resolution analysis of chromosome markers in burkitt lymphoma cell lines

Abstract

Using a high-resolution chromosome banding technique, which provided more elongated and distinctly banded chromosomes, some new evidence was obtained for localization of break points in Burkitt lymphoma marker chromosomes. As a result, the characteristic translocation between chromosomes 8 and 14 was designated as t(8;14) (q24.1;q32.5) and the deletion of chromosome 15 was designated as del(15) (q13q15).

Elongated mouse chromosomes suitable for enhanced molecular cytogenetics

“…Over the past three decades, several approaches were developed to produce elongated 'high resolution' human chromosomes by using intercalating agents (Zhang et al 1982). These agents included ethidium bromide (3,8-diamino-5-ethyl-6-phenylphenanthridium bromide) that interferes with chromatin structure (Paoletti 1979) due to its intercalation properties, and also by the electrostatic effects of its quaternary nitrogen in position 5 of the molecule (Paoletti 1979;Tomiyasu and Testa 1988;Lemke et al 2001).…”

In memoriam: Prof. Dr. rer. nat. Dr. med. h.c. Lore Zech; 24.9.1923 – 13.3.2013: Honorary member of the European Society of Human Genetics, Honorary member of the German Society of Human Genetics, Doctor laureate, the University of Kiel, Germany

“…The description of the Burkitt translocation was the basis to understand that oncogenes are activated by juxtaposition with regulatory sequences, i.e. by translocating the C-MYC oncogene into the immunoglobulin heavy chain locus [3,4]. Likewise, the identification of small deletions like the deletion of band 13q14 in B-chronic lymphocytic leukemia (B-CLL) was later recognized to harbor an important tumor suppressor gene [5].…”