2023
DOI: 10.1101/2023.02.15.528626
|View full text |Cite
Preprint
|
Sign up to set email alerts
|

High-resolution assessment of multidimensional cellular mechanics using label-free refractive-index traction force microscopy

Abstract: A critical requirement for studying cell mechanics is three-dimensional (3D) assessment of cellular shapes and forces with high spatiotemporal resolution. Traction force microscopy (TFM) with fluorescence imaging enables the measurement of cellular forces, but it is limited by photobleaching and a slow 3D acquisition speed. Here, we present refractive-index traction force microscopy (RI-TFM), a high-speed volumetric technique that simultaneously quantifies the 3D morphology and traction force of cells. RI-TFM … Show more

Help me understand this report
View published versions

Search citation statements

Order By: Relevance

Paper Sections

Select...
1

Citation Types

0
1
0

Year Published

2023
2023
2024
2024

Publication Types

Select...
1
1

Relationship

0
2

Authors

Journals

citations
Cited by 2 publications
(1 citation statement)
references
References 61 publications
0
1
0
Order By: Relevance
“…ODT is particularly attractive because it obtains both intensity and phase information using off-axis holography. Recently, it has found various biological applications, including measuring cell dry mass, 9 flow cytometry, 10 traction force microscopy, 11 and 3D histopathology. 12 Accelerating the acquisition speed of ODT would enable fast 3D studies of single-cellular swimmers and active colloidal suspensions on previously inaccessible time scales.…”
Section: Introductionmentioning
confidence: 99%
“…ODT is particularly attractive because it obtains both intensity and phase information using off-axis holography. Recently, it has found various biological applications, including measuring cell dry mass, 9 flow cytometry, 10 traction force microscopy, 11 and 3D histopathology. 12 Accelerating the acquisition speed of ODT would enable fast 3D studies of single-cellular swimmers and active colloidal suspensions on previously inaccessible time scales.…”
Section: Introductionmentioning
confidence: 99%