High resolution bands in maize chromosomes by G-banding methods

Kakeda, Katsuyuki; Yamagata, Hirotada; Fukui, Kiichi; Ohno, Minoru; Wei, Zhou; Zhu, Erle

doi:10.1007/bf00224397

Cited by 19 publications

(6 citation statements)

References 26 publications

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“…The chromosomes of the inbred lines Oh43 (29) and KYS (9, 10) have been characterized for the position of heterochromatic knobs, 5S sequence, size, arm ratio, and the presence of the secondary constriction (chromosome 6). Based on these and other articles that describe the distribution of the sequences of Cent4 (21) and TR-1 (23), the FISH signal distributions on Oh43 of the repeated sequences used were determined as follows (Fig.…”

Section: Resultsmentioning

confidence: 99%

Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize

Kato

Lamb

Birchler

2004

Proc. Natl. Acad. Sci. U.S.A.

476

499

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Study of the maize (Zea mays L.) somatic chromosomes (2n ‫؍‬ 20) has been difficult because of a lack of distinguishing characteristics. To identify all maize chromosomes, a multicolor fluorescence in situ hybridization procedure was developed. The procedure uses tandemly repeated DNA sequences to generate a distinctive banding pattern for each of the 10 chromosomes. Fluorescence in situ hybridization screening trials of nonsubtracted or subtracted PCR libraries resulted in the isolation of microsatellite 1-26-2, subtelomeric 4-12-1, and 5S rRNA 2-3-3 clones. These three probes, plus centromeric satellite 4 (Cent4), centromeric satellite C (CentC), knob, nucleolus-organizing region (NOR), pMTY9ER telomereassociated sequence, and tandemly repeated DNA sequence 1 (TR-1) were used as a mixture for hybridization to root-tip chromosomes. All 10 chromosomes were identified by the banding and color patterns in the 14 examined lines. There was significant quantitative variation among lines for the knob, microsatellite, TR-1, and CentC signals. The same probe mixture identifies meiotic pachytene, late prophase I, and metaphase I chromosomes. The procedure could facilitate the study of chromosomal structure and behavior and be adapted for other plant species.

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Section: Resultsmentioning

confidence: 99%

Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize

Kato

Lamb

Birchler

2004

Proc. Natl. Acad. Sci. U.S.A.

476

499

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“…Chromosome G-banding patterns represent natural conformational features of chromatin (Saitoh and Laemmli 1994;Hoshi and Ushiki 2001;Oberringer et al 2004). In plants, although some reports on G-banding have been published (Drewary 1982;Schubert et al 1984;Murata and Orton 1984;Wang and Kao 1988;Yang and Zhang 1988;Kakeda et al 1990;Peffley and de Vries 1993;Song et al 1994;Muravenko et al 1999Muravenko et al , 2003, a satisfactory Gbanding technique, suitable for different plant species, has not yet been established. In particular, closely adjacent multiple fluorescence bands, including Q-bands and similar have never been resolved.…”

Section: Introductionmentioning

confidence: 95%

A new chromosome fluorescence banding technique combining DAPI staining with image analysis in plants

Liu

She

et al. 2004

Chromosoma

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In this study, a new chromosome fluorescence banding technique was developed in plants. The technique combined 4',6-diamidino-2-phenylindole (DAPI) staining with software analysis including three-dimensional imaging after deconvolution. Clear multiple and adjacent DAPI bands like G-bands were obtained by this technique in the tested species including Hordeum vulgare L., Oryza officinalis, Wall & Watt, Triticum aestivum L., Lilium brownii, Brown, and Vicia faba L. During mitotic metaphase, the numbers of bands for the haploid genomes of these species were about 185, 141, 309, 456 and 194, respectively. Reproducibility analysis demonstrated that banding patterns within a species were stable at the same mitotic stage and they could be used for identifying specific chromosomes and chromosome regions. The band number fluctuated: the earlier the mitotic stage, the greater the number of bands. The technique enables genes to be mapped onto specific band regions of the chromosomes by only one fluorescence in situ hybridisation (FISH) step with no chemical banding treatments. In this study, the 45S and 5S rDNAs of some tested species were located on specific band regions of specific chromosomes and they were all positioned at the interbands with the new technique. Because no chemical banding treatment was used, the banding patterns displayed by the technique should reflect the natural conformational features of chromatin. Thus it could be expected that this technique should be suitable for all eukaryotes and would have widespread utility in chromosomal structure analysis and physical mapping of genes.

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“…Although maize chromosomes can be individually identified based on their morphology (McClintock 1929), there are ambiguities. Chromosome banding techniques were developed in 1980s and 1990s for maize chromosome identification (de Carvalho andSaraiva 1993, 1997;Deaguiarperecin and Vosa 1985;Jewell and Islam-Faridi 1994;Kakeda et al 1990;Rayburn et al 1985;Ward 1980). C-banding, the most popular banding technique in plants, revealed mostly the knob-related regions on maize chromosomes (Deaguiarperecin and Vosa 1985;Jewell and Islam-Faridi 1994;Rayburn et al 1985).…”

Section: Introductionmentioning

confidence: 99%

A universal chromosome identification system for maize and wildZeaspecies

Braz

Martins

Zhang

et al. 2020

Preprint

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Maize was one of the first eukaryotic species in which individual chromosomes can be identified cytologically, which made maize one of the oldest models for genetics and cytogenetics research. Nevertheless, consistent identification of all 10 chromosomes from different maize lines as well as from wild Zea species remains a challenge. We developed a new technique for maize chromosome identification based on fluorescence in situ hybridization (FISH). We developed two oligonucleotide-based probes that hybridize to 24 chromosomal regions. Individual maize chromosomes show distinct FISH signal patterns, which allow universal identification of all chromosomes from different Zea species. We developed karyotypes from three Zea mays subspecies and two additional wild Zea species based on individually identified chromosomes. A paracentric inversion was discovered on the long arm of chromosome 4 in Z. nicaraguensis and Z. luxurians based on modifications of the FISH signal patterns. Chromosomes from these two species also showed distinct distribution patterns of terminal knobs compared to other Zea species. These results support that Z. nicaraguensis and Z. luxurians are closely related species. ReferencesAdawy SSM, Stupar RM, Jiang J (2004) Fluorescence in situ hybridization of knob-associated DNA elements analysis reveals multiple loci in one-knob and knobless maize lines. J (2019) Whole-chromosome paints in maize reveal rearrangements, nuclear domains, and chromosomal relationships. P Natl Acad Sci USA 116:1679-1685 Amarillo FIE, Bass HW (2007) A transgenomic cytogenetic sorghum (Sorghum propinquum) bacterial artificial chromosome fluorescence in situ hybridization map of maize (Zea mays L.) pachytene chromosome 9, evidence for regions of genome hyperexpansion.

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High resolution bands in maize chromosomes by G-banding methods

Cited by 19 publications

References 26 publications

Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize

Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize

A new chromosome fluorescence banding technique combining DAPI staining with image analysis in plants

A universal chromosome identification system for maize and wildZeaspecies

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