High resolution emission spectra of one second delayed fluorescence from chloroplasts

Hideg, Éva; Scott, R. Q.; Inaba, Humio

doi:10.1016/0014-5793(89)80737-9

Cited by 18 publications

(2 citation statements)

References 24 publications

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“…The origin of intrinsic low level chemiluminescence in plant materials in darkness is not yet completely understood (see Abeles, 1986 for review). It differs from the delayed fluorescence observed in green plant tissue, which is attributed to recombination reactions in the electron transport system of the photosynthetic unit (Amesz et al, 1978;Hideg et al, 1989) and occurs tens of minutes after exposure to light. The role of activated oxygen species, dioxetanes, carbonyls, carbonic radicals, lipids, oxidases, fatty acids and nucleic acids have been discussed (Abeles, 1986;Boveris et al, 1983;Scott et al, 1989a).…”

Section: Introductioncontrasting

confidence: 58%

Induction of a Transient Enhancement of Low Level Chemiluminescence in Intact Leaves by Anaerobic Treatment

Roschger¹,

Devaraj²,

Scott

et al. 1992

Photochem & Photobiology

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Abstract— Intrinsic low level chemiluminescence of dark adapted intact leaves exhibits a transient enhancement of light emission when ambient air is replaced with an anaerobic atmosphere. Re establishing aerobic conditions gives rise to a complex kinetic behavior of the light emission in the post‐anoxic phase. Metabolic changes within the leaf are suggested to be responsible for this phenom enon, where intactness of the leaf is found to be of crucial importance. Leaf homogenate showed nearly oxygen independent chemiluminescence. In contrast, low level chemiluminescence of UV‐light damaged leaves and of chloroplast preparations was inhibited by oxygen depletion. Spectral analyses of the ultraweak light emission from the intact leaf indicates that in both aerobic and anaerobic cases, the source of the ultraweak light is the same. The similarity to the spectrum of delayed fluorescence also obtained from the leaf suggests that chlorophyll is the light emitting species.

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Section: Introductioncontrasting

confidence: 58%

Induction of a Transient Enhancement of Low Level Chemiluminescence in Intact Leaves by Anaerobic Treatment

Roschger¹,

Devaraj²,

Scott

et al. 1992

Photochem & Photobiology

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“…Interestingly, the feature at 685 nm is composed of two emissions of different broadening, which we tentatively assign to chlorophyll molecules bound to different proteins. A fuller description of this work is to appear elsewhere (Hideg et al, 1989). The effects of fluctuations (arising from the quantum detection process and source fluctuations) are evident when the spectra of Figs 4(a) and 4(b) are compared.…”

Section: High-resolution Ultraweak Spectroscopymentioning

confidence: 99%

Single photon counting imagery

Scott¹,

Inaba

1989

J. Biolumin. Chemilumin.

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Advent of the multichannel plate and position sensitive detector has made possible true single photon counting imaging tubes. We have investigated the application of these detectors in studies of the ultraweak light emission of biological materials. Initially, we focussed our efforts on t w o objectives: 1) obtaining single photon counting images of living tissues using only the light (chemiluminescence) emitted by the specimen and 2) developing means of obtaining well-resolved spectra of weakly emitting sources. We have obtained a variety of images. One striking result of this work is the first observation of tissue specific localization of photon emission in situ. Using this detector we have also obtained the first well-resolved spectra of some important ultraweak emission processes. These results illustrate the potential use of single photon imaging in bioluminescence and chemiluminescence research.

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