2003
DOI: 10.1007/s00122-003-1557-9
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High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley

Abstract: The dominant gene Rdg2a of barley conferring resistance to the hemi-biotrophic seed-borne pathogen Pyrenophora graminea is located in the distal region of chromosome arm 1 (7H)S. As the first step towards isolating the gene, a high-resolution genetic map of the region was constructed using an F(2) population of 1,400 plants (Thibaut Rdg2axMirco). The map included six classes of resistance gene analogues (RGAs) tightly associated with Rdg2a. Rdg2a was delimited to a genetic interval of 0.14 cM between the RGAs … Show more

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Cited by 33 publications
(19 citation statements)
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“…The Rdg2a locus resides in a chromosome region of high recombination [7], which is a characteristic that would assist in map-based cloning. To investigate the molecular basis of the Rdg2a -based P. graminea resistance in barley, map-based isolation of Rdg2a was initiated by constructing a high resolution genetic map representing 2,800 F 1 gametes.…”
Section: Resultsmentioning
confidence: 99%
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“…The Rdg2a locus resides in a chromosome region of high recombination [7], which is a characteristic that would assist in map-based cloning. To investigate the molecular basis of the Rdg2a -based P. graminea resistance in barley, map-based isolation of Rdg2a was initiated by constructing a high resolution genetic map representing 2,800 F 1 gametes.…”
Section: Resultsmentioning
confidence: 99%
“…To investigate the molecular basis of the Rdg2a -based P. graminea resistance in barley, map-based isolation of Rdg2a was initiated by constructing a high resolution genetic map representing 2,800 F 1 gametes. The locus was delimited to a 0.14 cM marker interval, and a PCR-based marker located 0.07 cM from Rdg2a was developed [7].…”
Section: Resultsmentioning
confidence: 99%
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“…The rate of meiotic crossovers can vary more than tenfold across regions of a few hundreds of kilobases (kb; Okagaki and Weil 1997;Inukai et al 2000;Stein et al 2000;Wei et al 2002;Bulgarelli et al 2004;Drouaud et al 2007). In Arabidopsis, recombination hotspots reveal frequencies higher than 100 cM/Mb (Drouaud et al 2007) and are located in either coding or non-coding regions, showing that meiotic recombination is not necessarily associated with coding regions.…”
Section: Introductionmentioning
confidence: 99%