High-Resolution Imaging of the Middle Ear With Optical Coherence Tomography

Pitris, Costas; Saunders, Kathleen; Fujimoto, James G.; Brezinski, Mark E.

doi:10.1001/archotol.127.6.637

Cited by 72 publications

(54 citation statements)

References 23 publications

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“…OCT relies on differences in tissue optical properties to generate contrast, with axial resolution on the order of 10 m and of a depth of penetration, of approximately 1-2 mm, depending on turbidity. Most OCT applications in the study of the middle and inner ear have focused on either animal investigations or human temporal bone studies [52][53][54].…”

Section: Article In Pressmentioning

confidence: 99%

Regeneration of chronic tympanic membrane perforation using 3D collagen with topical umbilical cord serum

Jang

Cho

Yeo

et al. 2013

International Journal of Biological Macromolecules

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Section: Article In Pressmentioning

confidence: 99%

Regeneration of chronic tympanic membrane perforation using 3D collagen with topical umbilical cord serum

Jang

Cho

Yeo

et al. 2013

International Journal of Biological Macromolecules

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“…Recently, several successful feasibility studies on optical coherence tomography (OCT) in middle ear research, both ex vivo (Pitris et al 2001;Bibas et al 2004) and in vivo (Djalilian et al 2008;Just et al 2009;Nguyen et al 2012) have prompted us to conduct preliminary tests to measure full-field thickness variations of several rabbit and human TMs with OCT . Though successful, it was clear that additional hardware and software modifications to the measurement setup were required in order to measure full membranes.…”

Section: Introductionmentioning

confidence: 99%

Full-Field Thickness Distribution of Human Tympanic Membrane Obtained with Optical Coherence Tomography

Jeught

Dirckx

Aerts

et al. 2013

JARO

124

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The full-field thickness distribution, three-dimensional surface model and general morphological data of six human tympanic membranes are presented. Crosssectional images were taken perpendicular through the membranes using a high-resolution optical coherence tomography setup. Five normal membranes and one membrane containing a pathological site are included in this study. The thickness varies strongly across each membrane, and a great deal of interspecimen variability can be seen in the measurement results, though all membranes show similar features in their respective relative thickness distributions. Mean thickness values across the pars tensa ranged between 79 and 97 μm; all membranes were thinnest in the central region between umbo and annular ring (50-70 μm), and thickness increased steeply over a small distance to approximately 100-120 μm when moving from the central region either towards the peripheral rim of the pars tensa or towards the manubrium. Furthermore, a local thickening was noticed in the antero-inferior quadrant of the membranes, and a strong linear correlation was observed between inferior-posterior length and mean thickness of the membrane. These features were combined into a single three-dimensional model to form an averaged representation of the human tympanic membrane. 3D reconstruction of the pathological tympanic membrane shows a structural atrophy with retraction pocket in the inferior portion of the pars tensa. The change of form at the pathological site of the membrane corresponds well with the decreased thickness values that can be measured there.

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“…The noninvasive, high-resolution depth-ranging and imaging capabilities of LCI/OCT offer the potential for applications in the middle ear, specifically for the detection and quantification of middle-ear biofilms (16,17). With micrometer-scale resolution, OCT is capable of imaging the microstructure of biofilms, including the dynamics of biofilm formation, as demonstrated in a laboratory flow-cell experiment in which the 3D structure was visualized over time (18).…”

mentioning

confidence: 99%

“…With micrometer-scale resolution, OCT is capable of imaging the microstructure of biofilms, including the dynamics of biofilm formation, as demonstrated in a laboratory flow-cell experiment in which the 3D structure was visualized over time (18). Three-dimensional OCT imaging has been performed on ex vivo human ear structures to reveal the microstructures of the middle ear, including the TM, malleus, incus, chorda tympani nerve, and tendon of the tensor tympani muscle (16). LCI data alone have been used to efficiently classifying human breast tissue types (19), suggesting that a similar application is possible to quantitatively characterize middle-ear structures and classify OM status.…”

mentioning

confidence: 99%

Noninvasive in vivo optical detection of biofilm in the human middle ear

Nguyen

Jung²,

Kim

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

101

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Otitis media (OM), a middle-ear infection, is the most common childhood illness treated by pediatricians. If inadequately treated, OM can result in long-term chronic problems persisting into adulthood. Children with chronic OM or recurrent OM often have conductive hearing loss and communication difficulties and require surgical treatment. Tympanostomy tube insertion, the placement of a small drainage tube in the tympanic membrane (TM), is the most common surgical procedure performed in children under general anesthesia. Recent clinical studies have shown evidence of a direct correspondence between chronic OM and the presence of a bacterial biofilm within the middle ear. Biofilms are typically very thin and cannot be recognized using a regular otoscope. Here we report the use of optical coherent ranging techniques to noninvasively assess the middle ear to detect and quantify biofilm microstructure. This study involves adults with chronic OM, which is generally accepted as a biofilm-related disease. Based on more than 18,537 optical ranging scans and 742 images from 13 clinically infected patients and 7 normal controls using clinical findings as the gold standard, all middle ears with chronic OM showed evidence of biofilms, and all normal ears did not. Information on the presence of a biofilm, along with its structure and response to antibiotic treatment, will not only provide a better fundamental understanding of biofilm formation, growth, and eradication in the middle ear, but also may provide much-needed quantifiable data to enable early detection and quantitative longitudinal treatment monitoring of middle-ear biofilms responsible for chronic OM.optical coherence tomography | primary care imaging | low-coherence interferometry | otoscopy | otolaryngology O titis media (OM) is a middle-ear infection often caused by bacteria growth between the tympanic membrane (TM) and the inner ear. Middle-ear infections occur in approximately 75% of children by age 3 y, and ear infections are the most common childhood illness treated by pediatricians (1, 2). Young children often acquire ear infections after upper respiratory tract infections or allergic reactions to foods or environmental allergens. Adults account for almost 20% of the annual visits for OM (3). Characterization of middle-ear infections is currently based on symptoms, duration, otoscopic observation, and physical diagnosis. The most common type is acute OM (AOM), a rapid-onset infection with one or more symptoms including otalgia, fever, and irritability. In AOM, physicians often see an abnormal-appearing TM on pneumatic otoscopy, including bulging, opacity, effusion, and decreased mobility. Most cases of AOM clear in 1 or 2 w, and antibiotics are frequently used. Recurrent AOM and OM with effusion are considered chronic OM. These diseases often cause long-term or permanent ear damage, which is linked to hearing loss and speech delay in small children if inadequately treated. Most cases of chronic OM do not respond to antibiotic treatment (4, 5), and thus surg...

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High-Resolution Imaging of the Middle Ear With Optical Coherence Tomography

Cited by 72 publications

References 23 publications

Regeneration of chronic tympanic membrane perforation using 3D collagen with topical umbilical cord serum

Regeneration of chronic tympanic membrane perforation using 3D collagen with topical umbilical cord serum

Full-Field Thickness Distribution of Human Tympanic Membrane Obtained with Optical Coherence Tomography

Noninvasive in vivo optical detection of biofilm in the human middle ear

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