High‐resolution in vivo imaging of human nailbed microvasculature by using photoacoustic microscopy

Abstract

Microcirculation imaging has significantly clinical value in early diagnosis and curative effect judgment of various diseases. The most superficial layer of the nailbed is rich in capillaries, which is suitable as a window on the microcirculation. However, few techniques can noninvasively observe the blood supply distribution of the nailbed, especially for high-resolution imaging of capillaries. In this study, we adapted an opticalresolution photoacoustic microscopy (OR-PAM) to image the nailbed microvasculature. The imaging sensitivity was significantly improved by hydration pretreatment of the nail. In vitro phantom experiments demonstrate that the sensitivity was improved about 3.5 times after hydration. In vivo imaging experiments of the nailbed microvasculature were conducted to further examine the enhanced sensitivity and practicability of OR-PAM. Moreover, the quantitative analysis of capillary loops showed that OR-PAM can extract the detection indicators including vascular morphology, diameter, and length, which provides a basis for clinical microcirculation detection using OR-PAM.

Light on Alzheimer’s disease: from basic insights to preclinical studies

“…Finally, the blood vessel tortuosity decreased in AD mice but steadily increased in WT mice (Figure 4G). The findings from this detection align with earlier studies through in vitro electron microscopy, suggesting that Aβ plaques adhere to and accumulates on the walls of meningeal <20 mJ/cm 2 (Li B. et al, 2023;Li D. et al, 2023).…”

“…This impact is assessed using transcranial Doppler ultrasound (TCD) ( Russell and Bornstein, 2005 ; Gaudet et al, 2010 ; Gasparovic et al, 2013 ; Yan et al, 2022 ). Furthermore, the blood–brain barrier (BBB) in brain can be unfolded by exploiting the microbubble contrast agents during focused ultrasound imaging processes ( Li B. et al, 2023 ; Li D. et al, 2023 ). This mechanism allows for neuroimmune modulation and controlled drug release, presenting a promising therapeutic avenue for AD ( Silva et al, 2018 ).…”

A review of a strategic roadmapping exercise to advance clinical translation of photoacoustic imaging: From current barriers to future adoption

“…More importantly, PAI is on the pathway for clinical translation into mainstream medicine [16] , [17] , with a variety of applications demonstrated in patients using the tomographic PAI geometry, with a substantial number of studies applying PAI for cancer visualisation, especially in breast cancer [18] , [19] , [20] , [21] . Recently, a wide range of potential further clinical applications have emerged, such as evaluation of skin microvasculature [22] , [23] , functional and endoscopic assessment of the gastrointestinal tract [24] , [25] , surgical guidance [26] , monitoring inflammation [27] , [28] , [29] and monitoring lymphadenopathies [30] / lymphedema [31] . These diverse applications demonstrate the future clinical potential for PAI underpinned by the versatile nature of the hardware implementations available.…”