The Nukhul Formation has been a point of controversy until now due to a lack of index fossils, varied facies modifications, and different time gaps. As a result, the calcareous nannofossils of the exposed Miocene rocks at Wadi Baba, west-central Sinai have been studied quantitatively for the first time. An important sequence of calcareous nannofossil bioevents has been recorded in the Nukhul Formation, including the FOs of Discoaster druggii, Sphenolithus dissimilis, Helicosphaera ampilaperta, and Sphenolithus disbelemnos, which correspond to the Aquitanian age of upper NN1 and NN2 zones. The Nukhul Formation contains four intervals that reflect eutrophic conditions in the nearshore habitats with substantial nutrient input from the terrigenous influx, as shown by the dominance of cool-water taxa, Coccoliths pelagicus and Reticulofenestra minuta. The lithofacies, microfacies types, abundance, dominance, and diversity of nannofossil assemblages classified the studied interval into two depositional sequences with four system tracts. These tracts refer to fluctuations in relative sea level changes in response to the eustatic sea-level curve.