High resolution, label‐free photoacoustic imaging of live chicken embryo developing in bioengineered eggshell

Sharma, Arunima; Ishak, Noreen; Swee‐Hin, Teoh; Pramanik, Manojit

doi:10.1002/jbio.201960108

Cited by 7 publications

(6 citation statements)

References 46 publications

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“…Most prominent to mention at this point are optical methods [116,117] including bioluminescence [118], fluorescence [119], and tomographic [120,121] techniques. Furthermore, ultrasonographic imaging [122], x-ray based tomographic [45] and even photoacoustic techniques [123] have been introduced, the latter of which with the potential for label-free imaging. In combination with single photon emission tomography (SPECT), tomographic x-ray techniques have been applied to initial evaluation of radiopharmaceuticals in chick embryos [19].…”

Section: Discussionmentioning

confidence: 99%

In vivo PET/MRI Imaging of the Chorioallantoic Membrane

et al. 2020

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The Hen's Egg Test Chorioallantoic Membrane (HET-CAM) of fertilized chick eggs represents a unique model for biomedical research. With its steadily increasing use, non-invasive in ovo imaging for longitudinal direct quantification of the biodistribution of compounds or monitoring of surrogate markers has been introduced. The full range of imaging methods has been applied to the HET-CAM model. From the current perspective, Magnetic Resonance Imaging (MRI) and Positron Emission Tomography (PET) appear promising techniques, providing detailed anatomical and functional information (MRI) and excellent sensitivity (PET). Especially by combining both techniques, the required sensitivity and anatomical localization of the signal source renders feasible. In the following, a review of recent applications of MRI and PET for in ovo imaging with a special focus on techniques for imaging xenotransplanted tumors on the CAM will be provided.

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Section: Discussionmentioning

confidence: 99%

In vivo PET/MRI Imaging of the Chorioallantoic Membrane

et al. 2020

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“…1-UST-PAT system was employed for phantom PAT imaging. 54 The schematic of the 1-UST-PAT system is shown in Fig. 2(i) .…”

Section: Methodsmentioning

confidence: 99%

High frame rate (∼3 Hz) circular photoacoustic tomography using single-element ultrasound transducer aided with deep learning

Rajendran

Pramanik

2022

J. Biomed. Opt.

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. Significance: In circular scanning photoacoustic tomography (PAT), it takes several minutes to generate an image of acceptable quality, especially with a single-element ultrasound transducer (UST). The imaging speed can be enhanced by faster scanning (with high repetition rate light sources) and using multiple-USTs. However, artifacts arising from the sparse signal acquisition and low signal-to-noise ratio at higher scanning speeds limit the imaging speed. Thus, there is a need to improve the imaging speed of the PAT systems without hampering the quality of the PAT image. Aim: To improve the frame rate (or imaging speed) of the PAT system by using deep learning (DL). Approach: For improving the frame rate (or imaging speed) of the PAT system, we propose a novel U-Net-based DL framework to reconstruct PAT images from fast scanning data. Results: The efficiency of the network was evaluated on both single- and multiple-UST-based PAT systems. Both phantom and in vivo imaging demonstrate that the network can improve the imaging frame rate by approximately sixfold in single-UST-based PAT systems and by approximately twofold in multi-UST-based PAT systems. Conclusions: We proposed an innovative method to improve the frame rate (or imaging speed) by using DL and with this method, the fastest frame rate of imaging is achieved without hampering the quality of the reconstructed image.

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“…Over the last two decades, PAI has been used for various preclinical and clinical applications. 14 These include imaging of the whole body (small animal), 64 brain, 61 , 66 , 74 breast, 73 , vasculature, 69 , 75 – 79 skin, 80 , 81 eye, 75 diabetic feet, 76 zebrafish, 48 chicken embryo, 82 etc. In addition, real-time temperature monitoring 83 and vulnerable plaque detection have also been demonstrated.…”

Section: Introductionmentioning

confidence: 99%

Looking deep inside tissue with photoacoustic molecular probes: a review

2022

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. Significance: Deep tissue noninvasive high-resolution imaging with light is challenging due to the high degree of light absorption and scattering in biological tissue. Photoacoustic imaging (PAI) can overcome some of the challenges of pure optical or ultrasound imaging to provide high-resolution deep tissue imaging. However, label-free PAI signals from light absorbing chromophores within the tissue are nonspecific. The use of exogeneous contrast agents (probes) not only enhances the imaging contrast (and imaging depth) but also increases the specificity of PAI by binding only to targeted molecules and often providing signals distinct from the background. Aim: We aim to review the current development and future progression of photoacoustic molecular probes/contrast agents. Approach: First, PAI and the need for using contrast agents are briefly introduced. Then, the recent development of contrast agents in terms of materials used to construct them is discussed. Then, various probes are discussed based on targeting mechanisms, in vivo molecular imaging applications, multimodal uses, and use in theranostic applications. Results: Material combinations are being used to develop highly specific contrast agents. In addition to passive accumulation, probes utilizing activation mechanisms show promise for greater controllability. Several probes also enable concurrent multimodal use with fluorescence, ultrasound, Raman, magnetic resonance imaging, and computed tomography. Finally, targeted probes are also shown to aid localized and molecularly specific photo-induced therapy. Conclusions: The development of contrast agents provides a promising prospect for increased contrast, higher imaging depth, and molecularly specific information. Of note are agents that allow for controlled activation, explore other optical windows, and enable multimodal use to overcome some of the shortcomings of label-free PAI.

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High resolution, label‐free photoacoustic imaging of live chicken embryo developing in bioengineered eggshell

Cited by 7 publications

References 46 publications

In vivo PET/MRI Imaging of the Chorioallantoic Membrane

In vivo PET/MRI Imaging of the Chorioallantoic Membrane

High frame rate (∼3 Hz) circular photoacoustic tomography using single-element ultrasound transducer aided with deep learning

Looking deep inside tissue with photoacoustic molecular probes: a review

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