High resolution light and electron microscopic localization of tubulin with the IGS (immuno gold staining) method

TRIM50 Protein Regulates Vesicular Trafficking for Acid Secretion in Gastric Parietal Cells

“…In density gradient centrifugation of the microsomal vesicles, TRIM50 was further enriched in the stimulation-associated vesicle fraction as demonstrated by its co-migration during the fractionation with the marker proteins, including H ϩ /K ϩ -ATPase and ezrin. During histamine-induced structural transformation of tubulovesicles into canaliculi connected with the apical surface, the subcellular localization of H ϩ /K ϩ -ATPase is dramatically changed to facilitate gastric acid secretion in parietal cells (23,25). Consistent with the previous observations, histamine induced a clear redistribution of H ϩ /K ϩ -ATPase from the cytoplasmic region to the apical side, leading to formation of dense immunoreactive spots near the cell periphery (Fig.…”

Localization by Immunogold Cytochemistry of Viral Antigen in Sections of Plant Cells Infected with Red Clover Mottle Virus

“…Resins (methacrylates-acrylates) polymerized at low temperature are superior to the standard epoxy resins in preserving native molecular structure and antigenicity of proteins (Carlemalm et al, 1982;Craig & Goodchild, 1982;Bendayan & Shore, 1982). Colloidal gold is an excellent marker for immunocytochemistry both for the electron microscope (Roth et al, 1978;De Mey et al, 1981 ; for virus antigen, see Garzon et al, 1982; for pea seed storage proteins, see Craig & Millerd, 1981 ;Craig & Goodchild, 1982) and the light microscope (De Mey et al, 1981;Roth, 1982). In this paper we describe immunogold methods that detect RCMV antigen in ultrathin and thick pea leaf sections after low temperature embedding.…”

“…HCl with trisodium citrate, ascorbic acid or white phosphorus respectively, essentially as described by Frens (1973) and Slot & Geuze (1981). Swine anti-rabbit immunoglobulin was coupled to 20 nm or to 5 nm colloidal gold by the method of De Mey et al ( 1981 ). Protein A was coupled to 8 nm colloidal gold after the method of Slot & Geuze (1981), except that the final solution was made up in 0.5 ~ ovalbumin in 0.05 M-Tris-saline.…”

Correct Targeting of the Bean Storage Protein Phaseolin in the Seeds of Transformed Tobacco

“…Affinity purified goat-antirabbit IgG was conjugated to lissamine rhodamine B (Polysciences Inc., Warrington, PA) according to the method of Brandtzaeg (7). Colloidal gold with an average particle diameter of 3 to 4 nm was prepared according to Keller et al (23), and affinity purified goat-anti-rabbit IgG adsorbed to the gold particles as described ( 15,16 16 h (13). Further processing was as described above with a 24-h polymerization.…”