High-resolution light microscopy for interpretation of renal biopsies

Hoffmann, Ernesto O.

doi:10.1007/bf00868738

Cited by 3 publications

(8 citation statements)

References 38 publications

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“…The sections are protected, and no discoloration or scratches have been noticed after 12 yr. In these sections, lesions of 0.2 p m (about the diameter of a mitochondrium) are easily recognized in the light microscope; therefore, diagnosis is possible directly from these sections, saving unnecessary further use of TEM (12)(13)(14)(15)(16). When TEM studies are required (structures 0.2 p m or less), the samples are obtained directly from the epoxy block used for HRLM and not from a separate blind sample.…”

Section: Discussionmentioning

confidence: 99%

“…Mincing the sample for TEM has been and probably still is necessary to obtain the best fixation for experimental material (16)(17)(18)(19). Mincing was originally introduced to facilitate fixation by osmium tetraoxide (osmic acid), an excellent but slow penetrating fixative (44).…”

Section: Discussionmentioning

confidence: 99%

“…A comprehensive evaluation of biologic material (HRLM, TEM, histochemistry, immunohistology, and insitu hybridization) is possible with EHT (11)(12)(13)(14)(15)(16)(49)(50)(51)(52)(53)(54)(55). However, as with other histology methods, there are inevitable limitations.…”

Section: Discussionmentioning

confidence: 99%

“…These included mitochondria, microbodies, desmosomes, tonofilaments, glomerular basement membrane, secretory granules (including neuroendocrine granules), mucin granules, bacteria, parasites, fungi, immune complexes, glomerular basement membrane, and others. The morphologic detail obtained with the described methods and the practical applications in surgical pathology were previously published (9)(10)(11)(12)(13)(14)(15)(16)(17).…”

Section: Tissue and Cell Preservationmentioning

confidence: 99%

“…These methods have added new details and dimensions to diagnostic pathology and histotechnology and are now used to solve problems in pathologic anatomy (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17). However, the sophisticated epoxy histotechnology (EHT) developed for ultrastructural research must be adapted * Address reprint requests to Ms. Flores for present demands of modem surgical pathology (18)(19)(20)(21).…”

Section: Introductionmentioning

confidence: 99%

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Improved High Resolution Light Microscopy and Transmission Electron Microscopy Techniques in Diagnostic Pathology

Flores¹,

Hoffmann²

1997

Journal of Histotechnology

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There is little argument concerning the usefulness of high resolution light microscopy (HRLM) and transmission electron microscopy (TEM) in diagnostic pathology. However, the sophisticated histotechnology, developed for ultrastructural research, can be adapted to cope with present demands of modem surgical pathology. In our laboratories we have introduced changes in handling the tissues to avoid blind, limited and fragmented samples. The use of fast penetrating, environment-resistant fixative has allowed larger samples. Embedding large biopsies in versatile epoxy Spurr has produced generous thick and thin sections, avoiding waste of diagnostic material and resulting in more accurate diagnosis. Better contrast of the sections was obtained by staining with basic fuchsin and toluidine blue. Coverslipping has prevented scratches and dirt on the sections. These adaptations of the methods for HRLM-TEM allow better utilization of light and electron microscopes for modern surgical pathology. (The J Histotechno1 20:45, 1997)

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Tissue and Cell Preservationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Improved High Resolution Light Microscopy and Transmission Electron Microscopy Techniques in Diagnostic Pathology

Flores¹,

Hoffmann²

1997

Journal of Histotechnology

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ACP Best Practice No 160. Renal biopsy specimens

Furness

2000

Journal of Clinical Pathology

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Taking a kidney biopsy is not a trivial procedure. The sample is almost invariably smaller than the pathologist would like. Investigation usually requires division into even smaller samples to permit the application of specialist techniques. In some cases the biopsy is taken not for diagnosis, but to assess the extent of tissue damage. The clinical need is sometimes extremely urgent. These features all underline the crucial importance of collaboration between pathologist and nephrologist if maximum benefit is to be obtained from such very small samples. Consequently, in deciding what to do with a renal biopsy, flexibility and thought are required rather than a single prescribed list of procedures. This article, written after extensive international consultation, represents an attempt to define current best practice in the laboratory handling of renal biopsy specimens, while not neglecting the need to tailor processing to the individual needs of each case. (J Clin Pathol 2000;53:433-438)

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Role of special stains as a useful complementary tool in the diagnosis of renal diseases: a case series study

Venkatesh

Malaichamy

2019

Int J Res Med Sci

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Background: Renal diseases constitute a major cause of morbidity in clinical practice and their incidence is on rise. Investigation usually requires division into even smaller samples to permit the application of specialized techniques.Methods: This is a prospective study conducted over a period of one year from January 2018 to December 2018 in the Department of Pathology, Coimbatore. A total of 58 renal biopsies were received from the Nephrology Department and the tissues were subjected to light microscopic and special stain studies.Results: Total 53 patients (91.38%) had high blood urea nitrogen value more than 20.0 mg/dl. 48 patients (82.76%) had high serum creatinine value more than 1.2 mg/dl. Out of 58 biopsy specimens, 46 (79.31%) showed primary glomerular lesions, 10 (17.24%) showed secondary glomerular lesion and 2 (3.45%) showed tubulointerstitial nephritis. Jones’s methanamine silver stain along with PAS stain helped in typing/staging of membranous glomerulopathy and membranoproliferative glomerulonephritis. Various changes in GBM like spike formation, thickening and moth-eaten appearance of GBM was noted which is seen in MGN stage II, IV and III respectively. Double contour and thickening of GBM was noted which is seen in type I and II MPGN respectively. In Myeloma cast nephropathy, tubular casts stained negative with Congo red which was used to differentiate it from amyloid deposition.Conclusions: The special stains used in this study helped in supplementing the light microscopic findings for diagnosis of kidney lesions. However, the use of other ancillary techniques like immunofluorescence and electron microscopy would help the pathologists in better and more accurate diagnosis.

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High-resolution light microscopy for interpretation of renal biopsies

Cited by 3 publications

References 38 publications

Improved High Resolution Light Microscopy and Transmission Electron Microscopy Techniques in Diagnostic Pathology

Improved High Resolution Light Microscopy and Transmission Electron Microscopy Techniques in Diagnostic Pathology

ACP Best Practice No 160. Renal biopsy specimens

Role of special stains as a useful complementary tool in the diagnosis of renal diseases: a case series study

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