High‐resolution localization of hyaluronic acid in the golden hamster oocyte‐cumulus complex by use of a hyaluronidase‐gold complex

Kan, Frederick W. K.

doi:10.1002/ar.1092280403

Cited by 31 publications

(28 citation statements)

References 56 publications

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“…Similar granules and filaments are present in the PVS of unfertilized mammalian oocytes after cumulus cell expansion, and both filaments and granules appear to attach to the oolemma (Dandekar et al, , 1995. Hyaluronan has also been localized ultrastructurally within the PVS and on the surface of the hamster oolemma using hyaluronidase conjugated to gold (Kan, 1990), consistent with the data obtained using ruthenium red. This granular filamentous matrix has been demonstrated in the PVS of rodents such as the hamster and mouse where it is relatively sparse , in humans ( Fig.…”

Section: Contents Of the Pvs Before Fertilizationsupporting

confidence: 72%

“…In ultrastructural studies, hyaluronan appears to be anchored to the oolemma as well as present in the PVS Kan, 1990). Somatic cell fusion can be inhibited by hyaluro- (Kujawa et al, 1986), and it is possible that the presence of hyaluronan in the PVS and on the oolemma could impede gamete membrane fusion.…”

Section: What Is the Significance Of The Matrix In The Pvs Of Unfertimentioning

confidence: 96%

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Perivitelline space: Does it play a role in blocking polyspermy in mammals?

Talbot

Dandekar

2003

Microscopy Res & Technique

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The perivitelline space of mammalian oocytes changes in size and composition during preimplantation development. Often overlooked in the past, this space contains a hyaluronan-rich extracellular matrix prior to fertilization and a cortical granule envelope following release of the cortical granules at fertilization. The hyaluronan-containing matrix of unfertilized oocytes is well developed in some species such as opossums and humans but is scant in rodents including the hamster and mouse. The significance of the hyaluronan-rich matrix, which attaches to the plasma membrane of the oocytes, is not fully understood. However, hyaluronan, which can inhibit membrane fusion, is present in the perivitelline space (PVS) of unfertilized oocytes and must be negotiated by the fertilizing sperm. Following fertilization, the cortical granule envelope forms as the cortical granules disperse, thereby causing the PVS to increase significantly in size. Calcium is important in the dispersion of the cortical granules following exocytosis. Once formed, the cortical granule envelope in some species is about the same thickness as the zona pellucida, but it is not readily visualized unless it is stained with fluorescent probes or examined ultrastructurally after following stabilization with ruthenium red. The envelope contains proteins that remain in the PVS until the time of blastocyst hatching. Although little work has been done on the functions of the cortical granule envelope, several studies are consistent with the idea that it plays a role in blocking polyspermy. While nicotine increases polyspermy in sea urchins, its effects on polyspermy in human smokers have not been characterized, but could be addressed in human in vitro fertilization labs.

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Section: Contents Of the Pvs Before Fertilizationsupporting

confidence: 72%

Section: What Is the Significance Of The Matrix In The Pvs Of Unfertimentioning

confidence: 96%

Perivitelline space: Does it play a role in blocking polyspermy in mammals?

Talbot

Dandekar

2003

Microscopy Res & Technique

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“…Thus, results from our lectin and monoclonal antibody studies indicate that the perivitelline space in the hamster is an unique extracellular compartment poor in glycoproteins. On the contrary, we have recently demonstrated by use of a hyaluronidase-gold complex that this region contains hyaluronic acid (Kan, 1990). The exact composition and functional significance of the perivitelline space require further studies.…”

Section: Cytochemical Properties Of the Perivitelline Spacementioning

confidence: 96%

Changes of glycoconjugate contents of the zona pellucida during oocyte growth and development in the golden hamster: A quantitative cytochemical study

Roux

Kan

1991

Anat. Rec.

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We demonstrated here with the high resolution lectin-gold approach and quantitative analysis, changes of glycoconjugates in the hamster zona pellucida (ZP) during oocyte growth and development in the ovary and the oviduct. Glycoconjugates which contain N-acetyl-D-galactosamine as terminal sugar residues are absent in the ovary but are secreted by secretory cells in the oviduct and are added to the ZP of superovulated oocytes during oviductal transit. Glycoconjugates which carry sialic acid as terminal sugar residues appear to be acquired mainly from the ovary. The oviduct contributes little of this particular component to the ZP during the transit of oocytes in the oviduct. On the contrary D-galactose and N-acetylglucosamine associated glycoconjugates, added to the ZP in ovarian follicles, are also secreted by non-ciliated oviductal epithelial cells and these secretory products are transferred to the ZP in significant amount during passage of the oocyte through the oviduct. Lectin-gold labeling of the ZP of superovulated oocytes reveals homogeneous distribution of gold particles throughout the zona matrix. Thus, we conclude that the ZP of hamster superovulated oocytes consists of glycoconjugates that may derive from different origins. Deposition of ZP glycocomponents begins in the ovary. Similar and new glycoconjugates, secreted by oviductal non-ciliated secretory cells, are added to the ZP of oocytes during oviductal transit. At this stage the ZP is made up of a homogeneous matrix of glycoconjugates.

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“…In addition, the mRNA of HAS3, which is an HA synthase, is present in porcine oocytes [20]. Moreover, when ultrathin sections of hamster cumulus-oocyte complex were treated with immunogold-labeled hyaluronidase, deposition of gold particles was observed in cumulus cells, the perivitelline space and between cumulus cells, but not in the zona pellucida [21]. From these findings, we considered that HA in the matrix of cumulus cells derives from the cumulus cells and that HA in the perivitelline space derives from oocytes.…”

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confidence: 99%

Amount of Hyaluronan Produced by Mouse Oocytes and Role of Hyaluronan in Enlargement of the Perivitelline Space

Ueno

Yoshida

Niimura

2009

Journal of Reproduction and Development

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Abstract. Production of hyaluronan (hyaluronic acid: HA) was demonstrated in denuded mouse oocytes (DOs) by the enzyme-linked immunosorbent assay, and the role of HA in enlargement of the perivitelline space in the oocytes was examined. The incidence of polyspermy following insemination was also observed in DOs in which HA synthesis was inhibited. HA was not detected in culture medium containing DOs immediately after collection. After culture for 7 h, 4.75 pg of HA per DO was detected in the medium, and the mean amount of HA significantly increased to 20.78 pg 14 h after culture. When DOs were cultured in medium containing 0.25 mM 4-methylumbelliferone (MU), an inhibitor of HA synthase, the mean amount of HA in the culture medium with DOs was 8.61 pg, which was significantly smaller than the amount in the control medium with non-treated DOs (21.59 pg). The mean size of the perivitelline space in oocytes cultured with cumulus cells (5.40 μm) did not differ from that (5.08 μm) of DOs. The mean size of the perivitelline space was significantly smaller in the MU-treated DOs (3.58 μm) than in the control DOs (4.65 μm). The fertilization rate did not differ between the MU-treated DOs (84.9%) and control DOs (81.0%), whereas the incidence of polyspermy was significantly higher in the MU-treated DOs (13.3%) compared with the control DOs (2.1%). These findings clarified that the HA involved in enlargement of the perivitelline space in oocytes is synthesized and secreted by the oocytes themselves. They also suggest that there is a close relationship between the size of perivitelline space and the incidence of polyspermy in mouse oocytes. Key words: Enzyme-linked immunosorbent assay, Hyaluronan production, Incidence of polyspermy, Mouse oocyte, Size of perivitelline space (J. Reprod. Dev. 55: [496][497][498][499][500][501] 2009) he perivitelline space is the gap between the plasma membrane and zona pellucida in oocytes. Recently, it has been reported that the incidence of polyspermy in porcine and mouse oocytes with larger perivitelline space is significantly lower than that in oocytes with a smaller perivitelline space [1][2][3][4]. Therefore, it has been suggested that there could be a relationship between the size of the perivitelline space and the incidence of polyspermy in porcine and mouse oocytes.It is known that glycosaminoglycans including hyaluronan (hyaluronic acid: HA) synthesized in cumulus cells by the stimulation of FSH and LH are secreted [5,6] and that the extracellular matrix in cumulus cells is occupied by the secreted HA [7][8][9][10]. HA has been found to also exist in the perivitelline space [11][12][13][14], and one of its properties is retention of large volumes of water [14]. Therefore, when HA accumulates in the perivitelline space, its role is to absorb water, which causes the perivitelline space to enlarge.On the other hand, with regard to the secretory source of HA in the perivitelline space, Salustri et al. [15] used [ 3 H] glucosamine as a metabolic precursor to examine the production of H...

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High‐resolution localization of hyaluronic acid in the golden hamster oocyte‐cumulus complex by use of a hyaluronidase‐gold complex

Cited by 31 publications

References 56 publications

Perivitelline space: Does it play a role in blocking polyspermy in mammals?

Perivitelline space: Does it play a role in blocking polyspermy in mammals?

Changes of glycoconjugate contents of the zona pellucida during oocyte growth and development in the golden hamster: A quantitative cytochemical study

Amount of Hyaluronan Produced by Mouse Oocytes and Role of Hyaluronan in Enlargement of the Perivitelline Space

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