Lakes actively store, transport, and transform a large quantity of carbon received from the terrestrial environment. Despite covering <4% of the global non-glaciated land surface (Verpoorter et al., 2014), they are hotspots of carbon processing (Cole et al., 2007;Drake et al., 2018;Tranvik et al., 2018). In most lake waters, dissolved organic matter (DOM) is the largest pool of organic carbon and represents an important energy source for heter otrophs (Logue et al., 2016). At northern latitudes that harbor the majority of the Earth's inland waters, lakes are typically ice covered in the winter (Figures 1a and 1b) (Wang et al., 2021). When surface water freezes, gases, ions, and stored DOM is expelled from the advancing ice-water interface during crystal growth into the underlying water (Belzile et al., 2002;Jørgensen et al., 2015;Petrich & Eicken, 2010). The process of freezing can also promote DOM flocculation (Santibáñez et al., 2019). As ice crystals form, some DOM may be flocculated and excluded from the ice crystal junctions, and the flocculated material may settle to the sediment (Belzile et al., 2002;Santibáñez et al., 2019). DOM and nutrients expelled from the lake ice provide niches for active biological communities in the underlying unfrozen water column (Grosbois & Rautio, 2018;Hampton et al., 2017). The retention characteristics of solutes and DOM during lake ice formation may depend on ice growth conditions, ambient temperature, and the chemical composition of DOM (Belzile et al., 2002;Santibáñez et al., 2019). Unlike solutes, microbes are preferentially incorporated into the overlying ice during progressive freezing (Santibáñez et al., 2019). To understand how winter ice formation affects DOM dynamics and ecosystem