High Resolution Mapping of RphMBR1012 Conferring Resistance to Puccinia hordei in Barley (Hordeum vulgare L.)

Fazlikhani, Leila; Keilwagen, Jens; Kopahnke, Doris; Deising, H. B.; Ordon, Frank; Perović, Dragan

doi:10.3389/fpls.2019.00640

Cited by 16 publications

(13 citation statements)

References 104 publications

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“…It was positioned at ∼22 kb in chromosome 1HS, slightly more distal than the Rph4 locus. This new gene (provisionally designated Rph MBR1012 ) segregated from Rph4 in an allelism test and will likely be designated as a new Rph gene (Fazlikhani et al., ).…”

Section: Resultsmentioning

confidence: 99%

Development of barley introgression lines carrying the leaf rust resistance genes Rph1 to Rph15

et al. 2020

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In many production areas, barley (Hordeum vulgare L.) is attacked by the leaf rust pathogen (Puccinia hordei Otth), a basidiomycetous fungus that reduces both its yield and quality. Many leaf rust resistance genes, known as reaction to P. hordei (Rph) genes, have been described in barley. To differentiate genetic variants for virulence in pathogen populations, plant pathologists use differentials (i.e., sets of host lines carrying different resistance genes). The sources of Rph1–15 were derived from cultivars, landraces, and wild barley (H. vulgare ssp. spontaneum K. Koch) accessions with diverse geographic origins and agromorphological traits. Ideal differential sets comprise single‐gene lines backcrossed to a single adapted accession that is susceptible to all known races of a pathogen. In this study, sources of Rph1–15 and other Rph gene donors were backcrossed to the susceptible barley cultivar Bowman and then genotyped to characterize the chromosomal positions and sizes of introgressions. Overall, 95 Bowman introgression lines for leaf rust resistance were developed and characterized for their rust phenotypes and genotypes. A single line was selected to represent each of the 15 Rph genes for use as the new barley leaf rust differential set. The existence of possible new resistance genes in the studied germplasm was postulated. The new Bowman Rph1–15 differential lines will facilitate the efficient virulence phenotyping of P. hordei and serve as valuable genetic stocks for Rph gene stacking and cloning in barley.

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Section: Resultsmentioning

confidence: 99%

Development of barley introgression lines carrying the leaf rust resistance genes Rph1 to Rph15

et al. 2020

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“…Anchoring of markers to physical position in the genome can also easily be accomplished with a simple BLASTN-search. In some cases, this approach might be sufficient [46].…”

Section: Discussionmentioning

confidence: 99%

GenoTypeMapper: graphical genotyping on genetic and sequence-based maps

et al. 2020

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Background The rising availability of assemblies of large genomes (e.g. bread and durum wheat, barley) and their annotations deliver the basis to graphically present genome organization of parents and progenies on a physical scale. Genetic maps are a very important tool for breeders but often represent distorted models of the actual chromosomes, e.g., in centromeric and telomeric regions. This biased picture might lead to imprecise assumptions and estimations about the size and complexity of genetic regions and the selection of suitable molecular markers for the incorporation of traits in breeding populations or near-isogenic lines (NILs). Some software packages allow the graphical illustration of genotypic data, but to the best of our knowledge, suitable software packages that allow the comparison of genotypic data on the physical and genetic scale are currently unavailable. Results We developed a simple Java-based-software called GenoTypeMapper (GTM) for comparing genotypic data on genetic and physical maps and tested it for effectiveness on data of two NILs that carry QTL-regions for drought stress tolerance from wild emmer on chromosome 2BS and 7AS. Both NILs were more tolerant to drought stress than their recurrent parents but exhibited additional undesirable traits such as delayed heading time. Conclusions In this article, we illustrate that the software easily allows users to display and identify additional chromosomal introgressions in both NILs originating from the wild emmer parent. The ability to detect and diminish linkage drag can be of particular interest for pre-breeding purposes and the developed software is a well-suited tool in this respect. The software is based on a simple allele-matching algorithm between the offspring and parents of a crossing scheme. Despite this simple approach, GTM seems to be the only software that allows us to analyse, illustrate and compare genotypic data of offspring of different crossing schemes with up to four parents in two different maps. So far, up to 500 individuals with a maximum number of 50,000 markers can be examined with the software. The main limitation that hampers the performance of the software is the number of markers that are examined in parallel. Since each individual must be analysed separately, a maximum of ten individuals can currently be displayed in a single run. On a computer with an Intel five processor of the 8th generation, GTM can reliably either analyse a single individual with up to 12,000 markers or ten individuals with up to 3,600 markers in less than five seconds. Future work aims to improve the performance of the software so that more complex crossing schemes with more parents and more markers can be analysed.

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“…Fresh uredospores for Ph isolate I-80 were propagated by using the highly susceptible barley cultivar Großklappige as previously described in Fazlikhani et al (2019). Seven-day-old Golden Promise plants were…”

Section: Rust Infectionmentioning

confidence: 99%

The barley HvSTP13GR mutant triggers resistance against biotrophic fungi

Skoppek

Punt

Heinrichs

et al. 2021

Molecular Plant Pathology

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Barley is not only one of the major crops to feed livestock and an essential ingredient of beer, it has also gained a growing importance in food production. However, infection with fungal pathogens is one of the most devastating threats to annual yields, causing severe damage to various organs (Liu et al., 2011;Park et al., 2015;Wolfe & McDermott, 2003). To ensure sustainable and high-yield crop production, an ongoing improvement towards resistant varieties is the most important challenge for research and plant breeding.An ecologically sustainable method of crop protection is the identification of, and breeding with, naturally occurring resistance genes that originate in related wild varieties or landraces (Dinh et al., 2020). However, many resistance genes are mainly effective against certain races of one fungal species or have already been

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High Resolution Mapping of RphMBR1012 Conferring Resistance to Puccinia hordei in Barley (Hordeum vulgare L.)

Cited by 16 publications

References 104 publications

Development of barley introgression lines carrying the leaf rust resistance genes Rph1 to Rph15

Development of barley introgression lines carrying the leaf rust resistance genes Rph1 to Rph15

GenoTypeMapper: graphical genotyping on genetic and sequence-based maps

The barley HvSTP13GR mutant triggers resistance against biotrophic fungi

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