High-Resolution MAS for Liquids and Semisolids

Keifer, Paul A.

doi:10.1002/9780470034590.emrstm1169

Cited by 3 publications

(6 citation statements)

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“…NMR spectroscopy is a highly reproducible, high‐throughput and non‐invasive tool for structural determination and quantitative analyses of metabolites in vitro and in vivo 18,19 . Proton nuclear magnetic resonance spectroscopy ( 1 H NMR) has been widely used for metabolomics due to its robust nature, natural abundance and minimal sample preparation 20–24 . The major disadvantage of NMR spectroscopy is its lower sensitivity compared to MS‐based techniques, with its detection limits ranging between micromolar to sub‐micromolar levels.…”

Section: Introductionmentioning

confidence: 99%

“…18,19 Proton nuclear magnetic resonance spectroscopy ( 1 H NMR) has been widely used for metabolomics due to its robust nature, natural abundance and minimal sample preparation. [20][21][22][23][24] The major disadvantage of NMR spectroscopy is its lower sensitivity compared to MS-based techniques, with its detection limits ranging between micromolar to sub-micromolar levels. However, recent developments in cryoprobes, nanoprobes and high magnetic field spectrometers have increased the sensitivity and resolution of NMR, increasing the number of detectable metabolites.…”

Section: Introductionmentioning

confidence: 99%

“…The cheaper instrument cost is another advantage of MS-based analysis over the NMR-based metabolomics approach. [15][16][17][18][19][20][21][22][23][24][25][26][27][28] Metabolomic strategies cover two approaches "untargeted discovery global" and "targeted validation tandem." 14,29 Untargeted discovery metabolomics is based on a hypothesis-generating approach.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, MS‐based analysis requires a small sample volume (as low as 10 µL) compared to NMR spectroscopy. The cheaper instrument cost is another advantage of MS‐based analysis over the NMR‐based metabolomics approach 15–28 …”

Section: Introductionmentioning

confidence: 99%

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Metabolomics of neurological disorders in India

Gupta

Sharma

2021

Analytical Science Advances

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Metabolomics is the comprehensive study of the metabolome and its alterations within biological fluids and tissues. Over the years, applications of metabolomics have been explored in several areas, including personalised medicine in diseases, metabolome‐wide association studies (MWAS), pharmacometabolomics and in combination with other branches of omics such as proteomics, transcriptomics and genomics. Mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy are the major analytical techniques widely employed in metabolomics. In addition, MS is coupled with chromatography techniques like gas chromatography (GC) and liquid chromatography (LC) to separate metabolites before analysis. These analytical techniques have made possible identification and quantification of large numbers of metabolites, encompassing characterization of diseases and facilitating a systematic and rational therapeutic strategy based on metabolic patterns. In recent years, the metabolomics approach has been used to obtain a deeper insight into the underlying biochemistry of neurodegenerative disorders and the discovery of biomarkers of clinical implications. The current review mainly focuses on an Indian perspective of metabolomics for the identification of metabolites and metabolic alterations serving as potential diagnostic biomarkers for neurological diseases including acute spinal cord injury, amyotrophic lateral sclerosis, tethered cord syndrome, spina bifida, stroke, Parkinson's disease, glioblastoma and neurological disorders with inborn errors of metabolism.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Metabolomics of neurological disorders in India

Gupta

Sharma

2021

Analytical Science Advances

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“…High-resolution magic-angle spinning (HR-MAS) NMR spectroscopy can be used to perform non-destructive metabolite profiling of tissue or other solid samples 70 . That means that after HR-MAS NMR spectroscopy, further testing of tissue samples can be performed such as histopathological evaluation, the current gold standard in disease diagnosis, or genome and protein sequencing.…”

Section: Nmr Spectroscopy Of Tissues and In Vivo Imaging Techniquesmentioning

confidence: 99%

Multiple markers for the non-invasive diagnosis and characterisation of prostate cancer

Roberts¹

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The early detection of prostate cancer (CaP), the most common internal cancer in men, is limited by an absence of biomarkers that accurately reflect clinically significant disease.Currently, the prostate cancer antigen 3 (PCA3) test in combination with TMPRSS2-ERG fusion gene detection in urine obtained following rectal prostatic massage represent arguably the best standalone biomarkers for CaP but are not widely used in comparison with serum prostate specific antigen (PSA). Limitations in CaP diagnosis and characterisation may be overcome by use of naturally-produced, prostate-specific biofluids, such as ejaculate and post-ejaculate urethral washings (PEUW). Ejaculate is known to contain prostatic cells and relevant molecules in the non-cellular component, while PEUW may contain these as well as biomarkers reflective of systemic changes due to or causing CaP.The overall aim of this thesis was to use molecular and metabolomic nuclear magnetic resonance (NMR)-based detection methods to study prostatic fluid derived from ejaculate and PEUW to improve CaP diagnosis. Specifically, this thesis sought to optimise ejaculate sample processing for metabolomics studies and evaluate the diagnostic performance of mRNA, microRNA and metabolomic markers in ejaculate to complement serum PSA in detecting clinically significant CaP. Furthermore, this thesis investigated the feasibility and performance of PEUW-based mRNA and metabolomic biomarker performance attributable to the presence of ejaculate in urine to reflect local prostatic and systemic alterations in order to more accurately detect clinically significant CaP. Dependent clinical variables considered were absolute (positive/negative) and clinically significant (present/absent) CaP as well as risk groups (low, intermediate, high) according to the D'Amico criteria.Using a NMR-based metabolomics enzyme kinetics study design, the addition of tartrate and cooling of ejaculate samples improved the stability of choline and phosphorylcholine concentrations. Sample collection into a sterile urine jar containing 5 mM (on-site) or 10 mM (off-site) tartrate in 20 ml PBS solution cooled to 277 K and cooled during transport until processing would result in at most a 2-3% change in choline and phosphorylcholine to facilitate sample collection off-site without significant effect on choline-based metabolites.Following prostatic cell RNA isolation, amplification and qPCR for β2-microglobulin (β2M), PSA, PCA3 and Hepsin in ejaculate, adequate RNA for all assays was obtained for 66 patients and determined that a Hepsin:PCA3 ratio in ejaculate together with serum PSA best predicted absolute CaP (AUC= 0.724 vs 0.676) and csCaP (AUC= 0.701 vs 0.680).iii Matched mRNA and microRNA expression was possible for a subgroup of patients (n=20) Lipids/lipoproteins dominated spectra of high grade samples. Overall CaP prediction using metabolites described in previous studies was not validated. However, findings suggest that incorporation of in vitro NMR-based metabolomics may translate to in v...

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Influence of Controlled Dipolar Interaction for Polymer-Coated Gd-Doped Magnetite Nanoparticles toward Magnetic Hyperthermia Application

Hazarika,

Borgohain,

Borah

2024

ACS Omega

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To maximize heat release from immobilized nanoparticles (NPs), a detailed understanding of the controlled dipolar interaction is essential for challenging magnetic hyperthermia (MH) therapies. To design optimal MH experiments, it is necessary to precisely determine magnetic states impacted by the inevitable concurrence of magnetic interactions under a common experimental form. In this work, we describe how the presence of dipolar interaction significantly alters the heating mechanism of host materials when NPs are embedded in them for MH applications. The concentration of the NPs and the intensity of their interaction can profoundly impact the amplitude and shape of the heating curves of the host material. The heating capability of interacting NPs might be enhanced or diminished, depending on their concentration within the host material. We propose chitosan-and dextran-coated Gd-doped Fe 3 O 4 NPs directing dipole interactions effective for the linear regime to enlighten the pragmatic trends. The outcomes of our study may have substantial implications for cancer therapy and could inspire novel approaches for maximizing the effectiveness of MH.

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High-Resolution MAS for Liquids and Semisolids

Cited by 3 publications

References 84 publications

Metabolomics of neurological disorders in India

Metabolomics of neurological disorders in India

Multiple markers for the non-invasive diagnosis and characterisation of prostate cancer

Influence of Controlled Dipolar Interaction for Polymer-Coated Gd-Doped Magnetite Nanoparticles toward Magnetic Hyperthermia Application

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