High resolution melting of trnL amplicons in fruit juices authentication

Faria, Miguel A.; Magalhães, André Luíz Rodrigues; Nunes, Eugénia; Oliveira, M. Beatriz P.P.

doi:10.1016/j.foodcont.2013.02.020

Cited by 53 publications

(34 citation statements)

References 25 publications

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“…Velázquez-Estrada et al (2013) revealed significant differences in some bioactive compounds (such as flavanone content) of fresh squeezed orange juice that undergone ultra-high pressure homogenisation (UHPH) treatments. All these alterations may have negative impacts on the (Faria et al 2013). However, owing to relative high price as well as short storage period of fresh juices, adulteration of fresh juices with cheaper or processed juices seems to be happening.…”

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confidence: 99%

Detection of adulteration in freshly squeezed orange juice by electronic nose and infrared spectroscopy

Shen¹,

Wu²,

Su³

et al. 2016

Czech J. Food Sci.

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Abstract:Shen F., Wu Q., Su A., Tang P., Shao X., Liu B. (2016): Detection of adulteration in freshly squeezed orange juice by electronic nose and infrared spectroscopy. Czech J. Food Sci., 34: 224-232.The use of electronic nose and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) as rapid tools for detection of orange juice adulteration has been preliminarily investigated and compared. Freshly squeezed orange juices were tentatively adulterated with 100% concentrated orange juices at levels ranging from 0% to 30% (v/v). Then the E-nose response signals and FTIR spectra collected from samples were subjected to multivariate analysis by principal component analysis (PCA) and linear discriminant analysis (LDA). PCA indicated that authentic juices and adulterated ones could be approximately separated. For the classification of samples with different adulteration levels, the overall accuracy obtained by LDA in prediction was 91.7 and 87.5% for E-nose and ATR-FTIR, respectively. Gas chromatography-mass spectrometry (GC-MS) results verified that there existed an obvious holistic difference in flavour characteristics between fresh squeezed and concentrated juices. These results demonstrated that both E-nose and FTIR might be used as rapid screening techniques for the detection of this type of juice adulteration.

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mentioning

confidence: 99%

Detection of adulteration in freshly squeezed orange juice by electronic nose and infrared spectroscopy

Shen¹,

Wu²,

Su³

et al. 2016

Czech J. Food Sci.

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“…The use of HRM combined with the discriminating power of barcoding has been reported recently in the food industry (Ganopoulos et al, 2012;Faria et al, 2013;Sakaridis et al, 2013). Here, we describe the development of the Bar-HRM technique as a rapid, accurate, and economical molecular method to identify seagrass species.…”

Section: Resultsmentioning

confidence: 99%

“…BAR-HRM has been shown to offer great potential in several uses related to species identification (Ganopoulos et al, 2012;Faria et al, 2013;Sakaridis et al, 2013) and here we developed this hybrid method to aid in the rapid and accurate identification of seagrass species. Two regions of the chloroplast genome, rbcL and rpoC, were used.…”

Section: Introductionmentioning

confidence: 99%

Rapid discrimination between four seagrass species using hybrid analysis

et al. 2015

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ABSTRACT. Biological species are traditionally identified based on their morphological features and the correct identification of species is critical in biological studies. However, some plant types, such as seagrass, are taxonomically problematic and difficult to identify. Furthermore, closely related seagrass species, such as Halophila spp, form a taxonomically unresolved complex. Although some seagrass taxa are easy to recognize, most species are difficult to identify without skilled taxonomic or molecular techniques. Barcoding coupled with High Resolution Melting analysis (BAR-HRM) offers a potentially reliable, rapid, and cost-effective method to confirm species. Here, DNA information of two chloroplast loci was used in combination with HRM analysis to discriminate four species of seagrass collected off the southern coast of Thailand. A distinct melting curve presenting one inflection point was generated for each species using rbcL primers. While the melting profiles of Cymodocea rotundata and Cymodocea serrulata were not statistically different, analysis of the M. Osathanunkul et al. 3958©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 3957-3963 (2015) normalized HRM curves produced with the rpoC primers allowed for their discrimination. The Bar-HRM technique showed promise in discriminating seagrass species and with further adaptations and improvements, could make for an effective and power tool for confirming seagrass species.

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“…The detection of the admixture of a different fruit could be easily detected although further studies should be performed in order to evaluate the method sensitivity. Recently, it has been demonstrated the efficacy of DNA barcoding with trnL as a target sequence combined with HRM analysis for the complete fruit species discrimination (i.e., orange, mango, peach, pear and pineapple) and their quantitative evaluation in fruit juices [41].…”

Section: Use Of Dna Barcoding and Genetic Traceability Of Plant-derivmentioning

confidence: 99%

DNA Barcoding as a Molecular Tool to Track Down Mislabeling and Food Piracy

2015

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DNA barcoding is a molecular technology that allows the identification of any biological species by amplifying, sequencing and querying the information from genic and/or intergenic standardized target regions belonging to the extranuclear genomes. Although these sequences represent a small fraction of the total DNA of a cell, both chloroplast and mitochondrial barcodes chosen for identifying plant and animal species, respectively, have shown sufficient nucleotide diversity to assess the taxonomic identity of the vast majority of organisms used in agriculture. Consequently, cpDNA and mtDNA barcoding protocols are being used more and more in the food industry and food supply chains for food labeling, not only to support food safety but also to uncover food piracy in freshly commercialized and technologically processed products. Since the extranuclear genomes are present in many copies within each cell, this technology is being more easily exploited to recover information even in degraded samples or transformed materials deriving from crop varieties and livestock species. The strong standardization that characterizes protocols used worldwide for DNA barcoding makes this technology particularly suitable for routine analyses required by agencies to safeguard food safety and quality. Here we conduct a critical review of the potentials of DNA barcoding for food labeling along with the main findings in the area of food piracy, with particular reference to agrifood and livestock foodstuffs.

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High resolution melting of trnL amplicons in fruit juices authentication

Cited by 53 publications

References 25 publications

Detection of adulteration in freshly squeezed orange juice by electronic nose and infrared spectroscopy

Detection of adulteration in freshly squeezed orange juice by electronic nose and infrared spectroscopy

Rapid discrimination between four seagrass species using hybrid analysis

DNA Barcoding as a Molecular Tool to Track Down Mislabeling and Food Piracy

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