During 2020, the stay-at-home order mandated in response to the COVID-19 pandemic led to significant changes in traffic volumes in Cincinnati, OH. Air pollutant concentrations (PM2.5, black carbon (BC), carbon monoxide (CO), nitrogen dioxide (NO2), nitrogen dioxide (NOX), and ozone (O3)) monitored at two ground monitoring sites in the city of Cincinnati were analyzed intra-annually in 2020 to quantify if the stay-at-home order impacted air quality. Interannual analyses were also conducted to evaluate differences in 2020 data versus historical years (2016–2019). Traffic volume data were also analyzed, where it was observed that, compared to pre-pandemic 2020, total traffic counts decreased by up to 26.41% during Ohio’s stay-at-home order, while heavy-duty vehicle traffic increased by up to 26.95% during the latter half of 2020. Statistical analysis indicated nonuniform changes in air pollutant concentrations at both sites throughout 2020. During the lockdown period at the central monitoring site, PM2.5 increased by 9%, while NO2 decreased by 30% compared to pre-pandemic concentrations in 2020. For BC and CO, there were no significant changes.