The Sea of Galilee in northeast Israel is a freshwater lake filling a morphological depression along the Dead Sea fault. it is located in a tectonically complex area, where a n-S main fault system intersects secondary fault patterns non-univocally interpreted by previous reconstructions. A set of multiscale geophysical, geochemical and seismological data, reprocessed or newly collected, was analysed to unravel the interplay between shallow tectonic deformations and geodynamic processes. the result is a neotectonic map highlighting major seismogenic faults in a key region at the boundary between the Africa/Sinai and Arabian plates. Most active seismogenic displacement occurs along nnW-SSe oriented transtensional faults. this results in a left-lateral bifurcation of the Dead Sea fault forming a rhomb-shaped depression we named the Capharnaum Trough, located off-track relative to the alleged principal deformation zone. Low-magnitude (M L = 3-4) epicentres accurately located during a recent seismic sequence are aligned along this feature, whose activity, depth and regional importance is supported by geophysical and geochemical evidence. this case study, involving a multiscale/ multidisciplinary approach, may serve as a reference for similar geodynamic settings in the world, where unravelling geometric and kinematic complexities is challenging but fundamental for reliable earthquake hazard assessments. Strike-slip deformation zones are complex tectonic domains generally showing high lateral variability. This is due mainly to strain partitioning, which can develop transtensive and transpressive deformations in response to local crustal heterogeneities or to changes in the regional stress field. Fault bends and oversteps create zones of diffuse deformation, which could mask the tracks of main active fault segments. On the other hand, diachronicity in fault activation-deactivation often creates complex patterns recording multiphase tectonic processes. Another uncertainty is whether or not deformations observed at the surface are expression of deep-seated tectonic structures. Where lakes or inland seas develop, particularly in tectonically subsiding areas, waterborne seismic reflection surveys can be a powerful tool to overcome these problems, because the relatively homogeneous and continuous sedimentary sequence which fills the depression may enhance imaging of tectonic structures, facilitating kinematic reconstructions. In the subaqueous environment, the good coupling between seismic source, substratum and receivers, together with a relative simplicity in field operations, permits acquisition of densely spaced grids of high-resolution subsurface images. Where deformation rates are high relative to sediment supply, exposed tectonic lineaments may be detected by combining seismic reflection profiles and high-resolution morpho-bathymetric maps. However, in order to produce reliable maps of active faults, these data should be coupled with other information, such as local seismicity, major historical earthquakes, geodetic ve...